Dog Interleukin 1β (IL- 1β) ELISA Kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

犬白介素1β(IL-1β)酶聯免疫試劑盒（CSB-E13836c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的IL1B含量。IL1B，即白介素-1β，是一種強效的促炎細胞因子，主要由單核細胞、巨噬細胞和樹突狀細胞產生。它在炎癥和免疫反應中起關鍵作用，通過激活IL-1受體和下游信號通路，調節細胞增殖、分化和凋亡。IL1B的研究對于理解炎癥性疾病的發生機制和開發治療策略具有重要意義。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，該產品適用于科研領域，如探究犬類模型中的炎癥性疾病病理過程、評估免疫調節劑或抗炎藥物的干預效果，以及動物疫苗研發中的免疫應答研究，為獸醫學和比較醫學領域的實驗提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

IL1BInterleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit
縮寫：

IL1B
Uniprot No.：

Q28292
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

7.8 pg/mL-500 pg/mL
靈敏度：

1.95 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	83-100
1:2	Average %	94
	Range %	91-99
1:4	Average %	95
	Range %	90-105
1:8	Average %	94
	Range %	86-100

回收率：

The recovery of dog IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-102
EDTA plasma (n=4)	97	90-104

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
亞細胞定位：

Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.
蛋白家族：

IL-1 family
數據庫鏈接：

STRING: 9615.ENSCAFP00000010762

UniGene: Cfa.33592