Dog Interleukin-13(IL13) ELISA kit

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產(chǎn)品詳情

產(chǎn)品描述：

犬白介素13(IL13)酶聯(lián)免疫試劑盒（CSB-EL011590DO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL13含量。IL13 是重要的細胞因子，在免疫調(diào)節(jié)等方面有重要作用。其異常表達與哮喘、特應性皮炎等多種疾病相關(guān)。研究機制主要聚焦于其與受體結(jié)合后激活下游信號通路，從而參與炎癥反應、細胞增殖分化等過程，是治療相關(guān)疾病的潛在靶點。試劑盒檢測范圍為12.5 pg/mL-800 pg/mL，適用于科研領(lǐng)域?qū)θ惷庖哒{(diào)節(jié)機制的研究。科研人員可將其應用于犬類過敏性疾病模型構(gòu)建、寄生蟲感染后免疫應答評估、藥物干預效果分析等研究，為探索犬類Th2型免疫反應提供可靠的工具支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

IL13 ELISA Kit; Interleukin-13 ELISA Kit; IL-13 ELISA Kit
縮寫：

IL13
Uniprot No.：

Q9N0W9
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

12.5 pg/mL-800 pg/mL
靈敏度：

3.12 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog IL13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	93-102
1:2	Average %	92
	Range %	88-96
1:4	Average %	90
	Range %	86-94
1:8	Average %	87
	Range %	82-91

回收率：

The recovery of dog IL13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	100-112
EDTA plasma (n=4)	85	80-90

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages.
亞細胞定位：

Secreted.
蛋白家族：

IL-4/IL-13 family
數(shù)據(jù)庫鏈接：

KEGG: cfa:442990

STRING: 9615.ENSCAFP00000001250

UniGene: Cfa.3566