Chicken Peptide YY(PYY) ELISA kit

產品詳情

文獻引用 (5) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

雞多肽YY(PYY)酶聯免疫試劑盒（CSB-EL019128CH）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PYY含量。PYY即肽YY，是腸道分泌的一種激素。它可參與調節食欲和能量平衡，通過與下丘腦等部位的受體結合，抑制食欲、減少食物攝入。目前圍繞其作用機制在肥胖、糖尿病等代謝性疾病治療方面開展了諸多研究，有望開發新型治療藥物。試劑盒檢測范圍為50 pg/mL-800 pg/mL，本試劑盒可廣泛應用于家禽營養學研究中攝食調控機制解析、飼料干預效果評估，以及病理模型中胃腸道功能變化等相關基礎研究，為禽類生理代謝研究提供可靠的技術支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

PYY ELISA Kit; Peptide YY-like ELISA Kit; PYY ELISA Kit
縮寫：

PYY
Uniprot No.：

P29203
種屬：

Gallus gallus (Chicken)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

50 pg/mL-800 pg/mL
靈敏度：

50 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken PYY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-99
1:2	Average %	97
	Range %	92-103
1:4	Average %	86
	Range %	80-89
1:8	Average %	90
	Range %	83-99

回收率：

The recovery of chicken PYY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	93	90-97

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility.
亞細胞定位：

Secreted.
蛋白家族：

NPY family