Chicken Interleukin 1β,IL-1β ELISA Kit

Chicken Interleukin 1β,IL-1β ELISA Kit

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文獻引用 (23) 說明書下載

中文名稱：

雞白介素1β(IL-1β)酶聯(lián)免疫試劑盒
貨號：

CSB-E11230Ch
規(guī)格：

96T/48T
價格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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引用文獻
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產品描述：

雞白介素1β(IL-1β)酶聯(lián)免疫試劑盒（CSB-E11230Ch）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL1B含量。IL1B 即白細胞介素-1β，是一種重要的促炎細胞因子。在炎癥反應、免疫調節(jié)等生理和病理過程中發(fā)揮關鍵作用。研究機制上，它可激活下游信號通路，引發(fā)炎癥級聯(lián)反應，與多種炎癥相關疾病如關節(jié)炎、心血管病等發(fā)病密切相關。試劑盒檢測范圍為0.27 pg/mL-200 pg/mL，特別適用于家禽免疫學研究、疫苗效力評估、感染性疾病模型建立等科研場景本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
縮寫：

IL-1β
Uniprot No.：

O73909
種屬：

Gallus gallus (Chicken)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.27 pg/mL-200 pg/mL
靈敏度：

0.27 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-100
1:2	Average %	99
	Range %	95-106
1:4	Average %	84
	Range %	80-88
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of chicken IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-98
EDTA plasma (n=4)	95	90-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
200	0.173	0.169	0.171
66.7	0.238	0.259	0.249
22.2	0.339	0.367	0.353
7.4	0.459	0.488	0.474
2.5	0.602	0.633	0.618
0.82	0.817	0.855	0.836
0.27	1.033	1.008	1.021
0	1.748	1.672	1.710

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Effect of Bile Acids Supplementation in Fatty Liver Hemorrhagic Syndrome, Production Performance, Physiological and Quality Characteristics of Laying Hen Eggs W Li,Animals,2024
Ameliorative effects of Sida acuta and vitamin C on serum DNA damage, pro-inflammatory and anti-inflammatory cytokines in roosters fed aflatoxin B1 contaminated diets OJ Olarotimi,Olarotimi O J,,2023
Production performance, immune indices, and caecal microbiota in broiler chickens fed diets supplemented with Morinda lucida leaf powder K Adeyemi,Tropical Agriculture,2023
Investigation of the Potential of Heterophil/Lymphocyte Ratio as a Biomarker to Predict Colonization Resistance and Inflammatory Response to Salmonella enteritidis Infection in Chicken M Thiam,Pathogens,2023
Effects of dietary supplementation with chlorogenic acid on growth performance, antioxidant capacity, and hepatic inflammation in broiler chickens subjected to diquat-induced oxidative stress P Zha,Poultry science,2023
Suppressive modulation of host immune responses by Dermanyssus gallinae infestation S Fujisawa,Poultry Science,2023
Comparative Analysis of the Liver Transcriptome of Beijing You Chickens and Guang Ming Broilers under Salmonella enterica Serovar Typhimurium Infection Z Wang,Microorganisms,2022
Influence of Dietary Supplementation of Guava Leaf, Oxytetracycline, and Tert-Butylhydroxytoluene on Growth Performance, Gut Microbial Population, Immune?… KD Adeyemi,Islamic Azad University,2022
Effects of different selenium sources on duodenum and jejunum tight junction network and growth performance of broilers in a model of fluorine-induced chronic oxidative stress S Chen,Poultry science,2022
Clostridium butyricum alone or combined with 1, 25‐dihydroxyvitamin D3 improved early‐stage broiler health by modulating intestinal flora Y Yu,Journal of Applied Microbiology,2021
Influence of Anacardium occidentale leaf supplementation in broiler chicken diet on performance, caecal microbiota, blood chemistry, immune status, carcass, and meat quality Foluke Sola-Ojo,British Poultry Science,2021
Enhanced cytokine expression and upregulation of inflammatory signaling pathways in broiler chickens affected by wooden breast myopathy T Xing,J. Sci. Food Agric,2020
Lack of Toxic Interaction Between Fusariotoxins in Broiler Chickens Fed throughout Their Life at the Highest Level Tolerated in the European Union Metayer JP, et al,Toxins,2019
Effects of sodium butyrate on intestinal health and gut microbiota composition during intestinal inflammation progression in broilers Zou X,Poultry Science,2019
Selenium Deficiency Aggravates Aflatoxin B1-Induced Immunotoxicity in Chick Spleen by Regulating 6 Selenoprotein Genes and Redox/Inflammation/Apoptotic Signaling Zhao L,The Journal of Nutrition,2019
Selenium Deficiency Aggravates Aflatoxin B1-Induced Immunotoxicity in Chick Spleen by Regulating 6 Selenoprotein Genes and Redox/Inflammation/Apoptotic Signaling Zhao L,The Journal of Nutrition,2019
Oxidative Stress in Microbial Diseases: Pathogen, Host, and Therapeutics Novaes RD, et al,Oxidative Medicine And Cellular Longevity,2019
Xylanase and Fermented Polysaccharide of Hericium caputmedusae Reduce Pathogenic Infection of Broilers by Improving Antioxidant and Anti-Inflammatory Properties Sitong Zhang, et al,Oxidative Medicine and Cellular Longevity,2018
The protective role of selenium against cadmium-induced hepatotoxicity in laying hens: Expression of Hsps and inflammation-related genes and modulation of elements homeostasis RunxiangZhang.et al,Ecotoxicology and Environmental Safety,2018
Dietary L-theanine alleviated lipopolysaccharide-induced immunological stress in yellow-feathered broilers RuiLi.et al,Animal Nutrition,2018
Effects of yeast cell wall on growth performance, immune responses and intestinal short chain fatty acid concentrations of broiler in an experimental necrotic enteritis model Guang-DaXue.et al,Animal Nutrition,2017
Immunization with avian metapneumovirus harboring chicken Fc induces higher immune responses. Paudel S.et al,Virus Res.,2016
The antimicrobial peptide sublancin ameliorates necrotic enteritis induced by in broilers S Wang. et al,Journal of Animal Science,2015

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.