Chicken Interleukin 17,IL-17 ELISA Kit
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中文名稱：雞白介素17(IL-17)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E04607Ch
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規(guī)格：96T/48T
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價(jià)格：￥3200/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：雞白介素17(IL-17)酶聯(lián)免疫試劑盒（CSB-E04607Ch）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IL17含量。IL-17是一種由Th17細(xì)胞分泌的促炎細(xì)胞因子，參與調(diào)節(jié)免疫反應(yīng)和炎癥過(guò)程。其在銀屑病、類風(fēng)濕關(guān)節(jié)炎等自身免疫疾病中起重要作用，同時(shí)也是腫瘤發(fā)生和進(jìn)展的關(guān)鍵因子。研究IL-17機(jī)制有助于開(kāi)發(fā)針對(duì)相關(guān)疾病的治療方法。試劑盒檢測(cè)范圍為150 pg/mL-6000 pg/mL，適用于家禽免疫學(xué)研究領(lǐng)域，如探究病原體感染后宿主免疫應(yīng)答動(dòng)態(tài)、評(píng)估疫苗免疫效果或解析炎癥性疾病模型中的細(xì)胞因子調(diào)控網(wǎng)絡(luò)，為禽類基礎(chǔ)免疫機(jī)制研究及疾病防控提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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縮寫(xiě)：IL-17
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Uniprot No.：
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種屬：Gallus gallus (Chicken)
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樣本類型：serum, plasma, tissue homogenates
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檢測(cè)范圍：150 pg/mL-6000 pg/mL
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靈敏度：150 pg/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Immunology
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測(cè)定原理：quantitative
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測(cè)定方法：Competitive
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精密度：
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 90 Range % 82-100 1:2 Average % 95 Range % 90-100 1:4 Average % 96 Range % 90-101 1:8 Average % 91 Range % 85-98 -
回收率：
The recovery of chicken IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 91 85-96 EDTA plasma (n=4) 92 86-98 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average 0 1.914 2.027 1.971 150 1.306 1.329 1.318 450 1.039 1.102 1.071 1380 0.755 0.702 0.729 3000 0.484 0.462 0.473 6000 0.351 0.348 0.350 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Evaluation of immunoprotective effects of recombinant protein and DNA vaccine based on Eimeria tenella surface antigen 16 and 22 in vivo Rui Fang,Parasitology Research,2021
- In vivo immunoprotective comparison between recombinant protein and DNA vaccine of Eimeria tenella surface antigen 4 P Zhao,Veterinary Parasitology,2020
- Molecular characterization and immune protection of an AN1-like zinc finger protein of Eimeria tenella Huanzhi Zhao, et al,Parasitology Research,2019
- The molecular characterization and protective efficacy of microneme 3 of Eimeria mitis in chickens XinmeiHuang.et al,Veterinary Parasitology,2018
- Molecular characterisation and the protective immunity evaluation of Eimeria maxima surface antigen gene Tingqi Liu.et al,Parasites & Vectors,2018
- The Molecular Characterization and Immunity Identification of Microneme 3 of Eimeria acervulina. Zhang Z.et al,J Eukaryot Microbiol.,2016
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