Chicken Insulin-like growth factor 1, IGF-1 ELISA Kit
-
中文名稱：雞胰島素樣生長因子1(IGF-1)酶聯(lián)免疫試劑盒
-
貨號：CSB-E09867Ch
-
規(guī)格：96T/48T
-
價(jià)格：￥3800/￥2500
-
其他：
產(chǎn)品詳情
-
產(chǎn)品描述：雞胰島素樣生長因子1(IGF-1)酶聯(lián)免疫試劑盒（CSB-E09867Ch）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IGF1含量。IGF1即胰島素樣生長因子1，在細(xì)胞的生長、增殖和分化等過程中扮演重要角色。研究機(jī)制方面，它主要通過與IGF1受體結(jié)合，激活下游信號通路，影響細(xì)胞的存活、代謝等。其異常表達(dá)與多種疾病如腫瘤、糖尿病等密切相關(guān)，是相關(guān)研究的重要靶點(diǎn)。試劑盒檢測范圍為125 pg/mL-2000 pg/mL，該產(chǎn)品可廣泛應(yīng)用于家禽生長發(fā)育機(jī)制研究、營養(yǎng)代謝實(shí)驗(yàn)、繁殖生理學(xué)分析以及激素調(diào)控相關(guān)的基礎(chǔ)科研領(lǐng)域，為實(shí)驗(yàn)室提供穩(wěn)定可靠的檢測工具。試劑盒配套包含標(biāo)準(zhǔn)品、檢測板、生物素標(biāo)記物及顯色試劑等完整組分，操作流程標(biāo)準(zhǔn)化，適用于生命科學(xué)、動(dòng)物醫(yī)學(xué)等領(lǐng)域的科研實(shí)驗(yàn)需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
-
別名：IGF1 ELISA Kit; Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit
-
縮寫：
-
Uniprot No.：
-
種屬：Gallus gallus (Chicken)
-
樣本類型：serum, plasma, tissue homogenates
-
檢測范圍：125 pg/mL-2000 pg/mL
-
靈敏度：125 pg/mL
-
反應(yīng)時(shí)間：1-5h
-
樣本體積：50-100ul
-
檢測波長：450 nm
-
研究領(lǐng)域：Signal Transduction
-
測定原理：quantitative
-
測定方法：Competitive
-
精密度：
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of chicken IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 91 Range % 86-96 1:2 Average % 97 Range % 92-105 1:4 Average % 87 Range % 85-90 1:8 Average % 96 Range % 93-99 -
回收率：
The recovery of chicken IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 90 85-94 EDTA plasma (n=4) 95 90-99 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average 2000 0.183 0.194 0.189 1000 0.311 0.333 0.322 500 0.526 0.500 0.513 250 1.024 1.028 1.026 125 2.107 2.120 2.114 -
數(shù)據(jù)處理：
-
貨期：3-5 working days
引用文獻(xiàn)
- Dynamic changes in insulin-like growth factor binding protein expression occur between embryonic and early post-hatch development in broiler chickens LA Vaccaro, K Herring, A Wilson, E England, AL Smith,Poultry Science,2024
- Dietary Clostridium butyricum and 25-Hydroxyvitamin D3 modulate bone metabolism of broilers through the gut–brain axis G Cao,Poultry science,2024
- Effects of dietary L-arginine on the production performance and gene expression of reproductive hormones in laying hens fed low crude protein diets VA Uyanga,Poultry science,2023
- Chitosan oligosaccharide as an effective feed additive to maintain growth performance, meat quality, muscle glycolytic metabolism, and oxidative status in yellow-feather broilers under heat stress Q Chang,Poultry Science,2020
- Dietary Supplementation of Purified Amino Acid Derived from Animal Blood on Immune Response and Growth Performance of Broiler Chicken T. G. Wandita.et al,Tropical Animal Science Journal,2018
產(chǎn)品評價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
-
功能：The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling.
-
基因功能參考文獻(xiàn)：
- It is a protein synthesis-related factor. PMID: 28594135
- The coding region of the IGF1 gene was polymorphic, expressed differentially during the pre-hatch and post-hatch periods, and haplogroups showed significant association with growth traits in chicken. PMID: 26059224
- The IGF-I gene is significantly associated with some growth traits (body weight at hatching, and at 4, 8, 12, and 14 weeks of age) in chickens. PMID: 23546986
- IGF1 single-nucleotide polymorphism c.47673G > A was associated with body weight and haematocrit percentage, and also with feed intake and percentages of abdominal fat and gizzard genotype x sex interactions. PMID: 23275255
- Insulin-like growth factor 1 stimulates Non-astrocytic Inner Retinal Glial cells to proliferate, migrate distally into the retina, and become reactive. PMID: 22973454
- The objective of this study was to examine associations of an IGF1 promoter polymorphism with growth, body composition, skeleton integrity, and metabolic traits in chickens. PMID: 15742956
- Genetic/nutritional models show a positive relation between muscle IGF-1 mRNA levels, which determine paracrine IGF-1 levels, and post hatch muscle growth. (Review) PMID: 16077194
- These results suggest that the mechanism of action whereby IGFBP2 excess impairs long bone development is to inhibit IGF-I and IGF-II mediated proliferation and matrix synthesis. PMID: 16183342
- Polymorphisms within the IGF1 gene are associated with 5-week body weight. PMID: 16734694
- Blood levels ofIGF-I may be more important than IGF-II after chicken hatching PMID: 17369538
- Data show that transcription level of IGF-1 decreased dramatically in the sex-linked dwarf. PMID: 17681929
- Association analysis showed that haplotypes based on three cIGF1 polymorphisms (c.-366A>C, c.528G>A and c.*1024C>T) were associated with body weight traits, suggesting that cIGF1 or a tightly linked gene had effects on body weight in the chicken. PMID: 18717967
- IGF-1 genes and proteins are expressed at high or moderately high levels in healing tendon PMID: 19084187
顯示更多
收起更多
-
亞細(xì)胞定位：Secreted.
-
蛋白家族：Insulin family
-
數(shù)據(jù)庫鏈接：
Most popular with customers
-
Human Transforming Growth factor β1,TGF-β1 ELISA kit
Detect Range: 23.5 pg/ml-1500 pg/ml
Sensitivity: 5.8 pg/ml
-
-
-
Mouse Tumor necrosis factor α,TNF-α ELISA Kit
Detect Range: 7.8 pg/ml-500 pg/ml
Sensitivity: 1.95 pg/ml
-
-
-
-
