Chicken Immunoglobulin of Yolk,IgY ELISA Kit

產品詳情

文獻引用 (6) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

雞卵黃免疫球蛋白(IgY)酶聯免疫試劑盒（CSB-E11635Ch）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、蛋黃樣本中的IgY含量。IgY即免疫球蛋白Y，是禽類血清和卵黃中的主要抗體。其背景在于可從卵黃高效提取，成本低、產量大。研究機制方面，IgY能特異性結合抗原，在抗感染、免疫診斷等領域展現作用，如中和病原體、阻斷其與宿主細胞結合等。試劑盒檢測范圍為0.078 ng/mL-5 ng/mL，靈敏度為0.02 ng/mL。該產品可廣泛應用于家禽免疫學研究、疫苗免疫效果評估、抗體生產工藝優化及生物制品質量控制等科研場景，為禽類相關疾病模型的免疫指標分析提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

IgY
種屬：

Gallus gallus (Chicken)
樣本類型：

serum, plasma, egg yolk
檢測范圍：

0.078 ng/mL-5 ng/mL
靈敏度：

0.02 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken IgY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:500000	Average %	89
	Range %	81-100
1:1000000	Average %	96
	Range %	87-103
1:2000000	Average %	97
	Range %	92-104
1:4000000	Average %	92
	Range %	86-98

回收率：

The recovery of chicken IgY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-105
EDTA plasma (n=4)	97	90-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

最新研究進展：

IgY，即鳥類IgY免疫球蛋白，是由鳥類產生的一種特殊類型的免疫球蛋白。最近的研究表明，IgY可以在多個領域中發揮重要作用，包括食品和醫藥工業。例如，IgY被用作生產抗體、免疫增強劑和生物傳感器的材料。此外，IgY還被用作一種潛在的治療腸道疾病的藥物。