Chicken Glutathione Peroxidase 8 (GPX-8) ELISA Kit

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產品詳情

產品描述：

雞谷胱甘肽過氧化物酶8(GPX-8)酶聯免疫試劑盒（CSB-E14942C）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GPX-8含量。雞谷胱甘肽過氧化物酶8（GPX-8）是一種位于內質網的非特異性抗氧化酶，參與清除活性氧，保護細胞膜免受氧化損傷。其基因突變與腫瘤發生密切相關，在多種腫瘤組織中異常表達，影響腫瘤免疫微環境和侵襲轉移。試劑盒檢測范圍為31.25 mU/mL-2000 mU/mL，該產品可廣泛應用于家禽氧化應激機制研究、營養代謝評估、毒理學實驗及疾病模型建立等科研領域，例如評估飼料抗氧化劑效果、監測環境毒素暴露對家禽抗氧化系統的影響，或探究傳染性疾病進程中GPX - 8的動態變化規律，為禽類健康管理和基礎醫學研究提供可靠的技術支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

GPX-8
Uniprot No.：

R4GGB2
種屬：

Gallus gallus (Chicken)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

31.25 mU/mL-2000 mU/mL
靈敏度：

7.8 mU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken GPX-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	98
	Range %	94-103
1:200	Average %	86
	Range %	83-90
1:400	Average %	104
	Range %	100-108
1:800	Average %	89
	Range %	85-93

回收率：

The recovery of chicken GPX-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	107	103-110
EDTA plasma (n=4)	84	80-88

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.