Canine S100 calcium binding protein A12/Calgranulin-C(S100A12) ELISA Kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

犬S100鈣結合蛋白A12/鈣粒蛋白C(S100A12)酶聯免疫試劑盒（CSB-E17221c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的S100A12含量。S100A12是一個靶點。它屬于S100蛋白家族，在炎癥反應等生理病理過程中起重要作用。研究機制方面，其可與細胞表面受體結合，激活多條信號通路，參與免疫調節、細胞增殖和遷移等過程，是疾病診斷和治療研究的潛在靶點。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，該產品適用于實驗動物模型中炎癥相關疾病的機制研究、藥物干預效果評估以及細胞實驗中蛋白表達水平動態監測，其多類型樣本兼容性為組織微環境分析、體外細胞模型研究及體液生物標志物探索提供可靠工具。為科研工作者解析S100A12在犬類疾病模型中的功能機制提供高效檢測方案。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

S100A12
Uniprot No.：

F1PMK3
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine S100A12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-89
1:2	Average %	96
	Range %	93-99
1:4	Average %	87
	Range %	82-91
1:8	Average %	97
	Range %	91-103

回收率：

The recovery of canine S100A12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	97	90-104

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














ng/ml	OD1	OD2	Average	Corrected
40	1.928	2.035	1.982	1.853
20	1.460	1.568	1.514	1.385
10	0.949	0.928	0.939	0.810
5	0.565	0.578	0.572	0.443
2.5	0.377	0.398	0.388	0.259
1.25	0.252	0.264	0.258	0.129
0.625	0.196	0.198	0.197	0.068
0	0.132	0.126	0.129