Canine N-terminal pro-brain natriuretic peptide(NT-proBNP)ELISA Kit

Canine N-terminal pro-brain natriuretic peptide(NT-proBNP)ELISA Kit

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中文名稱：

犬N端前腦鈉素(NT-proBNP)酶聯免疫試劑盒
貨號：

CSB-E17577c
規格：

96T/48T
價格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

犬N端前腦鈉素(NT-proBNP)酶聯免疫試劑盒（CSB-E17577c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的NT-proBNP含量。NT - proBNP是腦鈉肽原裂解后無活性的N端片段。其水平與心室壁張力及容量負荷密切相關，因此可作為反映心臟功能的生物標志物。臨床上常用于心力衰竭的診斷、病情評估及預后判斷，在心血管疾病研究中是重要靶點。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于犬血清或血漿樣本中心臟功能相關生物標志物的科研分析；常用于評估犬類心肌肥厚、充血性心力衰竭等疾病的病理機制研究；支持科研人員開展心血管疾病模型構建、藥物干預效果評估或病理生理機制研究，為犬類心臟疾病的實驗動物模型提供可靠的分子檢測工具；適用于實驗室常規檢測需求本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

NT-proBNP
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine NT-proBNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-92
1:2	Average %	97
	Range %	90-105
1:4	Average %	97
	Range %	92-104
1:8	Average %	93
	Range %	86-100

回收率：

The recovery of canine NT-proBNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	85-108
EDTA plasma (n=4)	97	92-106

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.675	2.655	2.665	2.516
10	2.152	2.144	2.148	1.999
5	1.311	1.302	1.307	1.158
2.5	0.781	0.799	0.790	0.641
1.25	0.377	0.363	0.370	0.221
0.625	0.264	0.254	0.259	0.110
0.312	0.186	0.190	0.188	0.039
0	0.146	0.152	0.149

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Serum Levels of Cardiac Markers NT-proANP and NT-proBNP in Brachycephalic bitches at Different Gestational Stages Feliciano M.et al,Reprod Domest Anim.,2016

■ 常見問題解答

Per customers request, please provide the following information regarding Canine N-terminal pro-brain natriuretic peptide, NT-proBNP ELISA Kit (CSB-E17577c)
Can this kit be used with cat samples too? Or is this kit only designed to be used for dogs?
1.What is the standard? (Recombinant full-length protein? Partial protein
(range?)?, peptide (sequence range?), native protein?)
2. If recombinant protein, then what is the host?
3. Is the capture antibody a monoclonal or polyclonal?
4. What is the host of the capture antibody?
5. What is the immunogen of the capture antibody?
6. Is the detection antibody a monoclonal or polyclonal?
7. What is the host of the detection antibody?
8. What is the immunogen of the detection antibody?

Thanks for your inquiry!
CSB-E17577c
This kit is specific to dog samples. It is not suggested to be used to test other species.
Capture antibody is mouse monoclonal antibody and immunogen is 293 cell-derived recombinant protein(full length).
Detection antibody is goat polyclonal antibody and immunogen is 293 cell-derived recombinant protein(fragment).
Standard is 293 cell-derived recombinant protein. Sorry we can not disclose the sequence.
Pls let me know if you have any further questions. Thank you.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.