Canine Fibroblast Growth Factor 23 (FGF23) ELISA Kit

產品詳情

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文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

犬成纖維細胞生長因子23(FGF23)酶聯免疫試劑盒（CSB-E17661c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的FGF23含量。FGF23（成纖維細胞生長因子23）是一種由骨骼和腎臟分泌的激素，主要參與調節磷酸鹽和鈣的代謝。其研究背景涉及慢性腎病、低磷血癥等疾病。FGF23通過與Klotho蛋白結合，調節腎小管對磷酸鹽的重吸收，影響維生素D代謝和甲狀旁腺激素的分泌。研究機制主要圍繞其與骨骼、腎臟和神經系統之間的相互作用，以及在不同疾病狀態下的功能變化。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，適用于科研領域中對犬類慢性腎病、鈣磷代謝紊亂及相關內分泌疾病的機制探索，亦可用于動物模型中骨礦化異常或激素調控路徑的基礎研究本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

ADHR, HPDR2, HYPF, PHPTC, tumor-derived hypophosphatemia inducing factor
縮寫：

FGF23
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.12 pg/mL-200 pg/mL
靈敏度：

0.78 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine FGF23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	95-101
1:2	Average %	87
	Range %	83-91
1:4	Average %	98
	Range %	94-102
1:8	Average %	84
	Range %	81-86

回收率：

The recovery of canine FGF23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-95
EDTA plasma (n=4)	93	90-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.