Canine Cardiac Troponin Ⅰ(cTn-Ⅰ) ELISA kit

Canine Cardiac Troponin Ⅰ(cTn-Ⅰ) ELISA kit

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中文名稱：

犬心肌肌鈣蛋白Ⅰ(cTn-Ⅰ)酶聯(lián)免疫試劑盒 Kit
貨號：

CSB-E08772c
規(guī)格：

96T/48T
價格：

￥4800/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

犬心肌肌鈣蛋白Ⅰ(cTn-Ⅰ)酶聯(lián)免疫試劑盒 Kit（CSB-E08772c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的cTn-I含量。cTn - I是心肌肌鈣蛋白I，是心肌損傷的特異性標(biāo)志物。其背景在于在心肌受損時釋放入血，可反映心肌損傷情況。研究機(jī)制主要聚焦在檢測其在血液中的含量變化，用于早期診斷急性心肌梗死等心肌損傷相關(guān)疾病。試劑盒檢測范圍為23.5 pg/mL-1500 pg/mL，本產(chǎn)品適用于科研領(lǐng)域，如犬類心肌病機(jī)制探索、心臟毒性藥物評價或運(yùn)動醫(yī)學(xué)研究中心肌微損傷監(jiān)測，為獸醫(yī)基礎(chǔ)研究、實(shí)驗(yàn)動物模型構(gòu)建及比較醫(yī)學(xué)分析提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

TNNI3 ELISA Kit; Troponin I ELISA Kit; cardiac muscle ELISA Kit; Cardiac troponin I ELISA Kit
縮寫：

cTn-
Uniprot No.：

Q8MKD5
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

23.5 pg/mL-1500 pg/mL
靈敏度：

5.8 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine cTn-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-100
1:2	Average %	89
	Range %	85-99
1:4	Average %	98
	Range %	91-104
1:8	Average %	96
	Range %	91-100

回收率：

The recovery of canine cTn-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-108
EDTA plasma (n=4)	95	90-101

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	2.245	2.250	2.248	2.071
750	1.716	1.704	1.710	1.533
375	1.144	1.161	1.153	0.976
187.5	0.790	0.775	0.783	0.606
94	0.459	0.474	0.467	0.290
47	0.305	0.289	0.297	0.120
23.5	0.215	0.211	0.213	0.036
0	0.176	0.177	0.177

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

功能：

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
基因功能參考文獻(xiàn)：
1. No statistical differences were found between CRP and cTnI levels, either before or after surgical correction of brachycephaly in dogs. PMID: 25673910
2. The objective of this study was to evaluate the correlation between anatomical components, clinical signs and several biomarkers, used to determine systemic inflammation and myocardial damage in dogs with brachycephalic upper airway obstructive syndrome. PMID: 22937913
3. The use of NT-pro-BNP and troponin I levels as prognostic indicators for canine heart failure is reported. PMID: 22439324
4. [NT-proBNP] concentration is significantly higher in dogs with pre-capillary pulmonary hypertension (PH) when compared to dogs with respiratory disease without PH; [NT-proANP] and [cTnI] were not elevated in dogs with pre-capillary PH. PMID: 21835711
5. Moderate exercise does not present a confounding variable in the interpretation of cardiac tropinin I and C-reactive protein concentrations in normal dogs. PMID: 20840303
6. Serum troponin I is high in dogs with either precapillary and postcapillary pulmonary hypertension (PH); myocardial damage in dogs with postcapillary PH is likely the consequence of increased mitral valve disease severity. PMID: 20002552
7. Analysis of troponin I concentration has potential to increase knowledge of overall cardiac remodeling in dogs with mitral valve disease; the effect of age on troponin I levels should be considered in these patients. PMID: 20002554
8. Circulating cTnI and pro-matrix metallopeptidase 9 increase in proportion to the severity of acute pulmonary thromboembolism PMID: 18047836
9. The incidence of myocardial injury due to ehrlichiosis, as demonstrated by elevated cardiac troponin I blood levels, is reported. PMID: 18638021
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蛋白家族：

Troponin I family
數(shù)據(jù)庫鏈接：

KEGG: cfa:403566

STRING: 9615.ENSCAFP00000003805

UniGene: Cfa.83

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.