Bovine α1-Acid glycoprotein,α1-AGP ELISA Kit

Bovine α1-Acid glycoprotein,α1-AGP ELISA Kit

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中文名稱：

牛α1酸性糖蛋白(α1-AGP)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E08579b
規(guī)格：

96T/48T
價(jià)格：

￥4200/￥3000
其他：

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產(chǎn)品描述：

牛α1酸性糖蛋白(α1-AGP)酶聯(lián)免疫試劑盒（CSB-E08579b）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的ORM1含量。ORM1 即阿片樣物質(zhì) μ 型受體 1，是一種與阿片類物質(zhì)作用相關(guān)的靶點(diǎn)。其背景上在痛覺(jué)調(diào)節(jié)等生理過(guò)程有重要地位。研究機(jī)制方面，主要圍繞其與配體結(jié)合，通過(guò)信號(hào)轉(zhuǎn)導(dǎo)通路發(fā)揮效應(yīng)，在鎮(zhèn)痛藥物研發(fā)等領(lǐng)域是重要研究對(duì)象。試劑盒檢測(cè)范圍為62.5 ng/mL-4000 ng/mL，該產(chǎn)品適用于科研領(lǐng)域，如評(píng)估牛類感染性疾病模型、炎癥相關(guān)藥效學(xué)研究、營(yíng)養(yǎng)代謝對(duì)免疫系統(tǒng)的影響，或通過(guò)組織勻漿分析特定器官在病理?xiàng)l件下的蛋白表達(dá)特征。為畜牧獸醫(yī)研究、動(dòng)物健康監(jiān)測(cè)及基礎(chǔ)醫(yī)學(xué)機(jī)制探索提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
別名：

ORM1 ELISA kit; AGPAlpha-1-acid glycoprotein ELISA kit; Orosomucoid ELISA kit; OMD ELISA kit
縮寫：

ORM1
Uniprot No.：

Q3SZR3
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

62.5 ng/mL-4000 ng/mL
靈敏度：

15.6 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine α1-AGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	102
	Range %	94-106
1:2000	Average %	92
	Range %	84-97
1:4000	Average %	101
	Range %	95-104
1:8000	Average %	97
	Range %	92-101

回收率：

The recovery of bovine α1-AGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	88-99
EDTA plasma (n=4)	92	88-96

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
4000	0.229	0.225	0.227
2000	0.450	0.459	0.455
1000	0.698	0.656	0.677
500	1.016	0.987	1.002
250	1.264	1.243	1.254
125	1.572	1.514	1.543
62.5	1.671	1.634	1.653
0	1.815	1.789	1.802

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Changes in the Concentrations of Acute Phase Proteins in Calves during the first Month of Life / Promene Koncentracije Proteina Akutne Faze Tokom Prvog Meseca 漚ivota Teladi Csilla T貿(mào)thov謾.et al,Acta Veterinaria,2015

功能：

Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Appears to function in modulating the activity of the immune system during the acute-phase reaction.
基因功能參考文獻(xiàn)：
1. The presence of lipopolysaccharide binding protein (LBP) and alpha1-acid glycoprotein (AGP) was demonstrated in all seven adipose tissue depots. Expression of AGP and LBP suggests that they may have roles as local and systemic inflammatory adipokines. PMID: 25542063
2. results provide evidence that locally produced alpha-1 acid glycoprotein may be involved in protecting sperm from phagocytosis by polymorphonuclear neutrophils in the bovine oviduct PMID: 24931131
3. Studied the regulation and immune function of AGP in cultured bovine oviduct epithelial cells. PMID: 25123565
4. As in human AGP and several other lipocalins, a temperature-induced molten globule state has been observed in the denaturation pathway of bovine AGP. PMID: 24530968
5. In the mitochondrial membrane, both AGP and MLQ proteins are almost quantitatively bound to ATP synthase. PMID: 17575325
6. Alpha1-acid glycoprotein isoforms that can be detected in bovine milk are reported. PMID: 17692138
7. study provides evidence that the expression of CD18 was downregulated by the plasma glycoforms of alpha-1 acid glycoprotein and inhibited the chemotaxis of monocytes PMID: 18577362
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亞細(xì)胞定位：

Secreted.
蛋白家族：

Calycin superfamily, Lipocalin family
數(shù)據(jù)庫(kù)鏈接：

KEGG: bta:497200

STRING: 9913.ENSBTAP00000022991

UniGene: Bt.49064

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.