Bovine luteinizing hormone (LH) ELISA kit

產品詳情

文獻引用 (8) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

牛黃體生成素（LH）酶聯免疫試劑盒（CSB-E12826B）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LH含量。LH即黃體生成素，是腺垂體分泌的促性腺激素。其主要作用于性腺，在女性中促進排卵和黃體形成，分泌孕激素；在男性中刺激睪丸間質細胞分泌雄激素。研究多聚焦其分泌調控機制及在生殖內分泌疾病診治中的應用。試劑盒檢測范圍為1.25 mIU/mL-100 mIU/mL，廣泛應用于動物繁殖生理機制研究、發情周期監測、生殖激素調控實驗等科研領域，亦可用于評估飼料添加劑或環境因素對牛群生殖功能的影響，為畜牧養殖優化和基礎醫學研究提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

LHBLutropin subunit beta ELISA kit; Luteinizing hormone subunit beta ELISA kit; LH-B ELISA kit; LSH-B ELISA kit; LSH-beta ELISA kit; Lutropin beta chain ELISA kit
縮寫：

LH
Uniprot No.：

P04651
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.25 mIU/mL-100 mIU/mL
靈敏度：

1.56 mIU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine LH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	99
	Range %	95-102
1:2	Average %	87
	Range %	84-90
1:4	Average %	88
	Range %	83-94
1:8	Average %	100
	Range %	95-105

回收率：

The recovery of bovine LH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	90-95
EDTA plasma (n=4)	87	85-92

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
亞細胞定位：

Secreted.
蛋白家族：

Glycoprotein hormones subunit beta family
數據庫鏈接：

KEGG: bta:280839

STRING: 9913.ENSBTAP00000048635

UniGene: Bt.12926