Bovine lipopolysaccharides (LPS) ELISA kit

產品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

牛脂多糖/內毒素(LPS)酶聯免疫試劑盒（CSB-EQ027975BO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LPS含量。LPS即脂多糖，是革蘭氏陰性菌細胞壁的主要成分。其具有免疫原性，可激活免疫系統。研究機制主要圍繞其與免疫細胞表面受體結合，觸發信號傳導通路，導致炎癥介質釋放等，在感染性疾病、炎癥相關研究中有重要意義。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于動物模型（如牛、小鼠等）中LPS水平的動態監測，例如評估細菌感染后內毒素釋放量、炎癥反應程度或藥物干預效果等研究；可為體外實驗或動物實驗提供可靠的數據支持，助力科研人員在免疫學、微生物學及藥理學等領域探索LPS相關分子機制。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

LPS
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine LPS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	99
	Range %	93-104
1:2	Average %	102
	Range %	98-110
1:4	Average %	90
	Range %	85-94
1:8	Average %	95
	Range %	90-99

回收率：

The recovery of bovine LPS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	80-90
EDTA plasma (n=4)	89	84-93

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.