Bovine Lysozyme (LZM) ELISA Kit

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產品詳情

產品描述：

牛溶菌酶(LZM)酶聯免疫試劑盒（CSB-E15829B）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LZM含量。牛溶菌酶（LZM）是一種具有抗菌、抗炎、抗病毒和免疫調節功能的堿性蛋白，其作用機制主要是通過裂解細菌細胞壁的肽聚糖結構，以及與病毒蛋白結合使其失活。近年來，研究者通過基因工程和轉基因技術，提高了LZM的表達量和活性，拓展了其在食品、醫藥和生物材料等領域的應用。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，支持科研人員探究反芻動物免疫調節機制、病原體防御反應等方向，例如在畜牧業中評估飼養環境對牛群免疫健康的影響，或基礎研究中解析LZM在組織局部抗感染過程中的動態表達規律，為動物模型構建及生物標志物研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

LYZ1Lysozyme C ELISA Kit; EC 3.2.1.17 ELISA Kit; 1,4-beta-N-acetylmuramidase C ELISA Kit
縮寫：

LZM
Uniprot No.：

P04421
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.156 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine LZM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	101
	Range %	96-105
1:400	Average %	90
	Range %	85-95
1:800	Average %	106
	Range %	100-110
1:1600	Average %	104
	Range %	99-109

回收率：

The recovery of bovine LZM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	87-98
EDTA plasma (n=4)	100	94-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
蛋白家族：

Glycosyl hydrolase 22 family
組織特異性：

Stomach-specific.
數據庫鏈接：

KEGG: bta:280849

UniGene: Bt.29367