Bovine Haptoglobin,Hpt/HP ELISA KIT

Bovine Haptoglobin,Hpt/HP ELISA KIT

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中文名稱：

牛結(jié)合珠蛋白/觸珠蛋白(Hpt/HP)酶聯(lián)免疫試劑盒
貨號：

CSB-E08585b
規(guī)格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

牛結(jié)合珠蛋白/觸珠蛋白(Hpt/HP)酶聯(lián)免疫試劑盒（CSB-E08585b）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的HP含量。牛結(jié)合珠蛋白（Haptoglobin，Hp）是一種在哺乳動物血清中廣泛存在的酸性糖蛋白，主要功能是與游離血紅蛋白結(jié)合，引導(dǎo)其進入降解代謝程序，防止其對機體的損傷。其亞型與多種疾病如感染、糖尿病、腫瘤等存在相關(guān)性，具有作為治療藥物的潛力。牛結(jié)合珠蛋白的分離純化方法包括硫酸銨沉淀、聚乙二醇沉淀等，這些方法有助于大規(guī)模純化結(jié)合珠蛋白，為后續(xù)的藥物開發(fā)提供材料。試劑盒檢測范圍為7.81 ng/mL-2000 ng/mL，該產(chǎn)品適用于畜牧科研中炎癥相關(guān)疾病的機制研究、疫苗或藥物干預(yù)效果評估，以及動物健康監(jiān)測等場景，為牛群疾病模型建立、營養(yǎng)代謝研究提供關(guān)鍵數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

HPHaptoglobin [Cleaved into: Haptoglobin alpha chain; Haptoglobin beta chain] ELISA Kit
縮寫：

HP
Uniprot No.：

Q2TBU0
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma
檢測范圍：

7.81 ng/mL-2000 ng/mL
靈敏度：

7.81 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine Hpt in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	84
	Range %	80-88
1:2000	Average %	90
	Range %	85-94
1:4000	Average %	103
	Range %	99-112
1:8000	Average %	98
	Range %	94-102

回收率：

The recovery of bovine Hpt spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	92	88-96

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
2000	0.243	0.252	0.248
500	0.412	0.436	0.424
120	0.793	0.754	0.774
31.25	1.214	1.239	1.227
7.81	1.795	1.724	1.760
0	2.405	2.359	2.382

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Tropical study on Clinical and Host Cell Responses of Acute Phase Proteins, Pro-Inflammatory Cytokines and Antibodies’ in Lactating Dairy Cows Vaccinated and Challenged with S. aureus IU Hambali,ResearchGate,2022
Enhancing dietary cation-anion difference reshaped the distribution of endotoxin across different biofluids and influenced inflammatory response in dairy cows … Z.Wang,Animal Feed Science and Technology,2020
Acute-phase responses in cattle infected with hydatid cysts and microbial agents. Sevimli A.et al,J Helminthol.,2015

功能：

As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
基因功能參考文獻：
1. These results indicate that it is possible to monitor the course of pyometra in cows based on the evaluation of the concentration of cytokines and Hp in the serum and uterine washings. PMID: 25704413
2. These data demonstrate that serum neutrophil haptoglobin-MMP 9 complexes appear sooner and decline more rapidly than other acute phase proteins. PMID: 25358728
3. An increase in serum amyloid A or haptoglobin in dairy cows with abomasal displacementis not specific for inflammation or bacterial infection; increased levels of either may indicate the presence of hepatic lipidosis. PMID: 20391639
4. Haptoglobin concentration may be a useful indicator for cows with postpartum reproductive disorders. PMID: 14960809
5. Presence in the milk of the acute phase proteins (APP), haptoglobin (Hp) and serum amyloid A to see the relationships in cow composite and bulk tank milk samples. PMID: 17227596
6. Bovine granulocytes, isolated from peripheral blood of healthy cattle, contain abundant amounts of haptoglobin within the granules. PMID: 17681384
7. This study suggests that neutrophils and epithelial cells may play an essential role in elevating milk haptoglobin (Hp) in addition to previous suggestions that Hp may be derived from mammary tissues and circulation. PMID: 19094922
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亞細(xì)胞定位：

Secreted, extracellular space.
蛋白家族：

Peptidase S1 family
組織特異性：

Expressed by the liver and secreted in plasma.
數(shù)據(jù)庫鏈接：

KEGG: bta:280692

STRING: 9913.ENSBTAP00000008335

UniGene: Bt.12553

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.