PRKN

PRKN，全名為parkin RBR E3泛素蛋白連接酶，也被稱為帕金蛋白。該蛋白是細胞內(nèi)重要的“質(zhì)檢員”，通過識別并標記異常蛋白質(zhì)，引導(dǎo)細胞清理系統(tǒng)清除受損組分，維持細胞內(nèi)環(huán)境的穩(wěn)定。PRKN蛋白參與多個生理過程，包括細胞對氨基酸刺激的響應(yīng)、神經(jīng)元死亡的負調(diào)控以及突觸傳遞的負調(diào)控。在神經(jīng)元死亡負調(diào)控的上游或內(nèi)部發(fā)揮作用，位于膜性器官的邊界膜、膜筏以及突觸后密度等細胞組件中，并與線粒體共定位。PRKN蛋白的研究對于理解帕金森病、2型糖尿病等疾病具有重要意義，其人類同源物與帕金森病、帕金森病2和卵巢癌有關(guān)。目前，針對PRKN蛋白的藥物研發(fā)尚處于早期階段，但已有研究表明，PRKN蛋白的異常可能與帕金森病的發(fā)生發(fā)展密切相關(guān)。

熱銷產(chǎn)品

Recombinant Human E3 ubiquitin-protein ligase parkin (PRKN) (CSB-BP017451HU)

驗證數(shù)據(jù)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

PRKN Recombinant Monoclonal Antibody (CSB-RA199844A0HU)

驗證數(shù)據(jù)

Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate(30μg), U-251MG whole cell lysate(30μg), Jurkat whole cell lysate(30μg), K562 whole cell lysate(30μg), HEK293 whole cell lysate(30μg), HEK293T whole cell lysate(30μg), SW620 whole cell lysate(30μg)
All lanes: Parkin antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 52 kDa
Observed band size: 30 kDa
Exposure time: 2min

Immunofluorescence staining of A-549 cell with CSB-RA199844A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing 786-O cells stained with CSB-RA199844A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

PRKN Antibody (CSB-PA017451LA01HU)

驗證數(shù)據(jù)

Western Blot
Positive WB detected in: HepG2 whole cell lysate, SH-SY5Y whole cell lysate, Jurkat whole cell lysate
All lanes: PRKN antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 52, 49, 24, 31, 43, 36, 44, 47 kDa
Observed band size: 52 kDa

IHC image of CSB-PA017451LA01HU diluted at 1:600 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA017451LA01HU diluted at 1:600 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescent analysis of PC-3 cells using CSB-PA017451LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

PRKN Antibody

PRKN Protein

PRKN Antibodies

PRKN for Homo sapiens (Human)

產(chǎn)品貨號	產(chǎn)品名稱	種屬反應(yīng)性	應(yīng)用類型
CSB-PA017451LA01HU	PRKN Antibody	Human	ELISA, WB, IHC, IF
CSB-PA076233	PARK2 Antibody	Human,Mouse,Rat	ELISA,WB,IHC
CSB-PA003708	PARK2 Antibody	Human	WB, IHC, ELISA
CSB-PA020235	Phospho-PARK2 (S131) Antibody	Human	WB, IHC, ELISA
CSB-PA020236	PARK2 Antibody	Human,Mouse,Rat	WB, IHC, IF, ELISA
CSB-RA199844A0HU	PRKN Recombinant Monoclonal Antibody	Human	ELISA, WB, IF, FC

PRKN Proteins

PRKN Proteins for Homo sapiens (Human)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP017451HU CSB-MP017451HU CSB-EP017451HU-B	Recombinant Human E3 ubiquitin-protein ligase parkin (PRKN)	Yeast Mammalian cell In Vivo Biotinylation in E.coli
CSB-EP017451HU	Recombinant Human E3 ubiquitin-protein ligase parkin (PRKN)	E.coli
CSB-BP017451HU	Recombinant Human E3 ubiquitin-protein ligase parkin (PRKN)	Baculovirus
CSB-BP017451HUb1	Recombinant Human E3 ubiquitin-protein ligase parkin (PRKN)	Baculovirus

PRKN Proteins for Rattus norvegicus (Rat)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP864348RA CSB-EP864348RA CSB-MP864348RA CSB-EP864348RA-B	Recombinant Rat E3 ubiquitin-protein ligase parkin (Park2)	Yeast E.coli Mammalian cell In Vivo Biotinylation in E.coli
CSB-EP864348RAb1	Recombinant Rat E3 ubiquitin-protein ligase parkin (Park2)	E.coli
CSB-BP864348RA	Recombinant Rat E3 ubiquitin-protein ligase parkin (Prkn)	Baculovirus

PRKN Proteins for Mus musculus (Mouse)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP893911MO CSB-BP893911MO CSB-MP893911MO CSB-EP893911MO-B	Recombinant Mouse E3 ubiquitin-protein ligase parkin (Prkn)	Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli