FOXA1
FOXA1，即叉頭框蛋白A1，別名HNF3α。FOXA1是一種在多個(gè)生物學(xué)過(guò)程中起核心作用的轉(zhuǎn)錄因子，包括器官發(fā)生、分化、糖脂代謝、細(xì)胞增殖、遷移和侵襲，以及藥物抗性。FOXA1通過(guò)結(jié)合到基因組上的特定區(qū)域，幫助打開(kāi)染色質(zhì)結(jié)構(gòu)，從而作為先鋒因子促進(jìn)其他轉(zhuǎn)錄因子的結(jié)合和基因的轉(zhuǎn)錄。在癌癥中，F(xiàn)OXA1與激素受體如雌激素受體ER和雄激素受體AR等相互作用，調(diào)控相關(guān)癌癥的發(fā)生、發(fā)展、轉(zhuǎn)移、耐藥等過(guò)程。FOXA1的突變或表觀遺傳修飾與多種癌癥的發(fā)展和治療抗性相關(guān)，尤其是在雌激素受體陽(yáng)性的乳腺癌和前列腺癌中。FOXA1還參與調(diào)節(jié)多種代謝途徑，包括糖代謝和脂代謝，影響細(xì)胞的代謝狀態(tài)和能量平衡。因此，F(xiàn)OXA1在維持細(xì)胞正常功能和促進(jìn)疾病進(jìn)展中扮演著關(guān)鍵角色，是癌癥治療中的一個(gè)重要靶點(diǎn)。
熱銷產(chǎn)品
Recombinant Human Hepatocyte nuclear factor 3-alpha (FOXA1) (CSB-EP008794HU)
驗(yàn)證數(shù)據(jù)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
FOXA1 Recombinant Monoclonal Antibody (CSB-RA246102A0HU)
驗(yàn)證數(shù)據(jù)
Western Blot
Positive WB detected in: MCF-7 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, Hela whole cell lysate
All lanes: FOXA1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 50, 46 kDa
Observed band size: 50 kDa
IHC image of CSB-RA246102A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA246102A0HU diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunoprecipitating FOXA1 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-RA246102A0HU in Hela whole cell lysate.
For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-RA246102A0HU(2μg)+ Hela whole cell lysate(500μg)
Lane 3: Hela whole cell lysate (10μg)
FOXA1 Antibodies
FOXA1 for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 種屬反應(yīng)性 | 應(yīng)用類型 |
|---|---|---|---|
| CSB-PA008794GA01HU | FOXA1 Antibody | Human,Mouse | ELISA,WB |
| CSB-PA000113 | Acetyl-FOXA1 (K264/253/211) Antibody | Human,Mouse,Rat | WB, ELISA |
| CSB-PA005476 | FOXA1 Antibody | Human,Mouse,Rat | WB, ELISA |
| CSB-PA132836 | FOXA1 Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
| CSB-PA008794LA01HU | FOXA1 Antibody | Human | ELISA, IHC |
| CSB-PA008794LB01HU | FOXA1 Antibody, HRP conjugated | Human | ELISA |
| CSB-PA008794LC01HU | FOXA1 Antibody, FITC conjugated | Human | |
| CSB-PA008794LD01HU | FOXA1 Antibody, Biotin conjugated | Human | ELISA |
| CSB-RA246102A0HU | FOXA1 Recombinant Monoclonal Antibody | Human | ELISA, WB, IHC, IP |
FOXA1 Proteins
FOXA1 Proteins for Mus musculus (Mouse)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來(lái)源 |
|---|---|---|
| CSB-YP008794MO CSB-EP008794MO CSB-BP008794MO CSB-MP008794MO CSB-EP008794MO-B |
Recombinant Mouse Hepatocyte nuclear factor 3-alpha (Foxa1) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
FOXA1 Proteins for Rattus norvegicus (Rat)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來(lái)源 |
|---|---|---|
| CSB-YP008794RA CSB-EP008794RA CSB-BP008794RA CSB-MP008794RA CSB-EP008794RA-B |
Recombinant Rat Hepatocyte nuclear factor 3-alpha (Foxa1) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
FOXA1 Proteins for Homo sapiens (Human)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來(lái)源 |
|---|---|---|
| CSB-YP008794HU CSB-BP008794HU CSB-MP008794HU CSB-EP008794HU-B |
Recombinant Human Hepatocyte nuclear factor 3-alpha (FOXA1) | Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-EP008794HU | Recombinant Human Hepatocyte nuclear factor 3-alpha (FOXA1) | E.coli |
FOXA1 Proteins for Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
| 產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來(lái)源 |
|---|---|---|
| CSB-YP804751XBF CSB-EP804751XBF CSB-BP804751XBF CSB-MP804751XBF CSB-EP804751XBF-B |
Recombinant Xenopus tropicalis Forkhead box protein A1 (foxa1) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
