Recombinant Human ATP-dependent RNA helicase DDX1 (DDX1)

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品名簡稱：

Recombinant Human DDX1 protein
貨號：

CSB-EP856393HU
說明書：

MSDS Datasheet
規(guī)格：

￥1536
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評價
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靶點詳情

產(chǎn)品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

DDX1
Uniprot No.：

Q92499
別名：

(DEAD box protein 1)(DEAD box protein retinoblastoma)(DBP-RB)
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

E.coli
分子量：

89.9 kDa
表達區(qū)域：

1-740aa
氨基酸序列

MAAFSEMGVMPEIAQAVEEMDWLLPTDIQAESIPLILGGGDVLMAAETGSGKTGAFSIPVIQIVYETLKDQQEGKKGKTTIKTGASVLNKWQMNPYDRGSAFAIGSDGLCCQSREVKEWHGCRATKGLMKGKHYYEVSCHDQGLCRVGWSTMQASLDLGTDKFGFGFGGTGKKSHNKQFDNYGEEFTMHDTIGCYLDIDKGHVKFSKNGKDLGLAFEIPPHMKNQALFPACVLKNAELKFNFGEEEFKFPPKDGFVALSKAPDGYIVKSQHSGNAQVTQTKFLPNAPKALIVEPSRELAEQTLNNIKQFKKYIDNPKLRELLIIGGVAARDQLSVLENGVDIVVGTPGRLDDLVSTGKLNLSQVRFLVLDEADGLLSQGYSDFINRMHNQIPQVTSDGKRLQVIVCSATLHSFDVKKLSEKIMHFPTWVDLKGEDSVPDTVHHVVVPVNPKTDRLWERLGKSHIRTDDVHAKDNTRPGANSPEMWSEAIKILKGEYAVRAIKEHKMDQAIIFCRTKIDCDNLEQYFIQQGGGPDKKGHQFSCVCLHGDRKPHERKQNLERFKKGDVRFLICTDVAARGIDIHGVPYVINVTLPDEKQNYVHRIGRVGRAERMGLAISLVATEKEKVWYHVCSSRGKGCYNTRLKEDGGCTIWYNEMQLLSEIEEHLNCTISQVEPDIKVPVDEFDGKVTYGQKRAAGGGSYKGHVDILAPTVQELAALEKEAQTSFLHLGYLPNQLFRTF
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Acts as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5' single-stranded RNA overhang nuclease activity. Possesses ATPase activity on various RNA, but not DNA polynucleotides. May play a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome. Together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation. Acts as a positive transcriptional regulator of cyclin CCND2 expression. Binds to the cyclin CCND2 promoter region. Associates with chromatin at the NF-kappa-B promoter region via association with RELA. Binds to poly(A) RNA. May be involved in 3'-end cleavage and polyadenylation of pre-mRNAs. Component of the tRNA-splicing ligase complex required to facilitate the enzymatic turnover of catalytic subunit RTCB: together with archease (ZBTB8OS), acts by facilitating the guanylylation of RTCB, a key intermediate step in tRNA ligation. Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of proinflammatory cytokines via the adapter molecule TICAM1. Specifically binds (via helicase ATP-binding domain) on both short and long poly(I:C) dsRNA.; (Microbial infection) Required for HIV-1 Rev function as well as for HIV-1 and coronavirus IBV replication. Binds to the RRE sequence of HIV-1 mRNAs.; (Microbial infection) Required for Coronavirus IBV replication.
基因功能參考文獻：
1. These results reveal an important role for DDX1 in the regulation of gene alternative splicing and insulin secretion in pancreatic beta cells. PMID: 29679569
2. LGR5 is a critical effector of DDX1 in colorectal cancer cells. PMID: 29869821
3. data have demonstrated that Venezuelan equine encephalitis virus (VEEV)-nsP3 associates with DDX1 and DDX3 in infected cells to facilitate viral multiplication; determined that VEEV-nsP3 interacts with a pre-formed translational complex, comprising of DX3:eIF4A:eIF4G:PABP to potentially aid in translation of viral proteins. PMID: 27105836
4. the two structural homologs FAM98A and FAM98B included in a novel complex with DDX1 and C14orf166 are required for PRMT1 expression in colorectal cancer cell lines PMID: 28040436
5. DDX1 binds HIV-1 rev response element to promote virus assembly. PMID: 28379444
6. DDX1, a RNA helicase also implicated in DSB repair, interacts with RIF1. Recruitment of DDX1 to DSBs is dependent on RIF1. DDX1 is also required for chromatin loading of BLM to ionizing radiation-induced DSBs. DDX1 and RIF1 have different nucleic acid requirements for accumulation at DSBs, with RNA-DNA hybrids required for DDX1 accrual at DSBs, and single-strand RNA required for accumulation of RIF1 at these sites. PMID: 28544931
7. Data provide evidence for a role for DDX1 in resolving RNA-DNA structures that accumulate at DNA double-strand breaks located at sites of active transcription thereby facilitating repair by homologous recombination. PMID: 27550810
8. crystal structure of the SPRY domain of human DDX1 (hDSPRY) is reported at 2.0 A resolution PMID: 26323305
9. HIV-1 wild type Tat co-immunoprecipitated with DDX1. PMID: 25496916
10. Data indicate a tight binding of DEAD-box protein DDX1 to adenosine diphosphate (ADP), one of the strongest affinities observed for DEAD-box helicases. PMID: 25690890
11. hCLE/C14orf166 associates with DDX1, HSPC117, and FAM98B in a novel transcription-dependent shuttling RNA-transporting complex. PMID: 24608264
12. archease (also called ZBTB8OS), a protein of unknown function, is required for full activity of the human tRNA ligase complex and, in cooperation with DDX1, facilitates the formation of an RTCB-guanylate intermediate central to mammalian RNA ligation PMID: 24870230
13. subcellular localization of KSRP is regulated by competing interactions with DDX1 or 14-3-3 PMID: 24023901
14. DDX1 depletion causes accumulation of DNA damage in response to cisplatin and defects in DNA repair of double-stranded breaks. PMID: 23797032
15. DDX1 was shown to be an RNA-activated ATPase, wherein Rev-bound RNA was equally effective at stimulating ATPase activity as protein-free RNA. PMID: 22051512
16. DDX1 acts as a cellular cofactor by promoting oligomerization of Rev on the Rev response element. PMID: 21763499
17. elevated levels of DDX1 RNA or the presence of DDX1 in the cytoplasm could serve as an effective prognostic biomarker for early recurrence in primary breast cancer. PMID: 20499159
18. DDX1 interacts with coronavirus nonstructural protein 14 and enhances viral replication. PMID: 20573827
19. determination as a homopolymeric poly(A) RNA-binding protein PMID: 12183465
20. These findings indicate that DDX1 is a critical cellular co-factor for Rev function, which maintains the proper subcellular distribution of this lentiviral regulatory protein. PMID: 15567440
21. DEAD box proteins and other RNA-binding proteins may play roles in active HIV replication and in the control of viral latency. PMID: 15588285
22. There may be a subset of NB in which enhanced DDX1 and low-NAG expression consequent to DDX1 co-amplification without NAG amplification contributes to susceptibility to intensive therapy. PMID: 17028906
23. Biotinylated DNA affinity precipitation and chromatin immunoprecipitation assays confirmed that DDX1 binds specifically to JCV-TCR. PMID: 17380053
24. DDX1 regulates proliferation of JCV in vitro through transcriptional activation. PMID: 17380054
25. Concomitant DDX1 and MYCN gain is associated with neuroblastoma PMID: 17611020
26. DDX1 plays an RNA clearance role at DNA double-strand breaks sites, thereby facilitating the template-guided repair of transcriptionally active regions of the genome. PMID: 18710941
27. DDX1 mRNA was produced in both seminoma and nonseminoma types of human testicular germ cell tumor samples PMID: 19398953
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亞細胞定位：

Nucleus. Cytoplasm. Cytoplasmic granule. Cytoplasm, cytosol. Mitochondrion.; Cytoplasm.
蛋白家族：

DEAD box helicase family, DDX1 subfamily
組織特異性：

Highest levels of transcription in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin including the retina, brain, and spinal cord.
數(shù)據(jù)庫鏈接：

HGNC: 2734

OMIM: 601257

KEGG: hsa:1653

STRING: 9606.ENSP00000233084

UniGene: Hs.440599