重組蛋白

重組蛋白質(zhì)是分析生物相互作用的有用工具。在研究實(shí)驗(yàn)室中使用了大量的重組蛋白質(zhì),包括商業(yè)蛋白質(zhì)和在特定研究項(xiàng)目中生成的蛋白質(zhì)。它們?cè)谠S多應(yīng)用中發(fā)揮著關(guān)鍵作用。
如果您正在尋找具有優(yōu)良質(zhì)量(驗(yàn)證的生物活性,較低的內(nèi)毒素水平和高純度)的重組蛋白質(zhì)來支持您的研究,您來對(duì)地方了。
經(jīng)過18年的發(fā)展,華美生物已經(jīng)建立了從原核到真核的五大系統(tǒng),用于表達(dá)重組蛋白質(zhì)。我們擁有一系列蛋白質(zhì)表達(dá)和純化技術(shù),包括基于桿狀病毒外膜蛋白(OMP)的蛋白質(zhì)表達(dá)技術(shù),以及膜蛋白表達(dá)技術(shù)等。
產(chǎn)品特點(diǎn)
- 超過13,000種重組蛋白覆蓋大多數(shù)基因/靶點(diǎn);
- 5種表達(dá)系統(tǒng),包括大腸桿菌(E.coli)、酵母、桿狀病毒感染的昆蟲細(xì)胞、哺乳動(dòng)物細(xì)胞和體外大腸桿菌。而體外大腸桿菌表達(dá)系統(tǒng)是華美生物的獨(dú)特表達(dá)系統(tǒng)。與傳統(tǒng)蛋白表達(dá)系統(tǒng)相比,體外大腸桿菌省略了許多過程,如質(zhì)粒轉(zhuǎn)化、細(xì)胞培養(yǎng)、收集、破碎和離心等,大大提高了工作效率。
- 多種標(biāo)簽,包括His、GST、Flag、MBP等。
- 廣泛應(yīng)用,包括抗原、細(xì)胞分析、結(jié)合分析/蛋白質(zhì)相互作用、細(xì)胞培養(yǎng)-無血清培養(yǎng)基、藥物相關(guān)研究、體外酶活性、ELISA標(biāo)準(zhǔn)及其原料、體內(nèi)研究、質(zhì)譜標(biāo)準(zhǔn)、蛋白芯片、SDS-PAGE控制、蛋白質(zhì)結(jié)構(gòu)分析(晶體/電子顯微鏡)。
- 多種物種,包括人類、小鼠、大鼠、兔子、豚鼠、雞、猴子、豬等。
- 無動(dòng)物副產(chǎn)品的動(dòng)物源性重組蛋白質(zhì)。華美生物已開發(fā)出無動(dòng)物副產(chǎn)品接觸的重組蛋白質(zhì)產(chǎn)品線。
重組蛋白目錄
以下是所有重組蛋白的目錄。您可以在目錄中找到適合您研究的蛋白質(zhì),或在本網(wǎng)站頭部的搜索框中搜索它們。
如果您找不到您需要的抗體,請(qǐng)查看華美生物蛋白質(zhì)定制服務(wù)。
通過選擇目標(biāo)名稱的首字母瀏覽產(chǎn)品。
產(chǎn)品名稱 | 貨號(hào) | 規(guī)格 |
---|---|---|
Recombinant Bacillus thuringiensis subsp. konkukian DNA-directed RNA polymerase subunit alpha (rpoA) | CSB-YP760773BAAE CSB-EP760773BAAE CSB-BP760773BAAE CSB-MP760773BAAE CSB-EP760773BAAE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Bacillus thuringiensis subsp. konkukian 50S ribosomal protein L30 (rpmD) | CSB-YP760774BAAE CSB-EP760774BAAE CSB-BP760774BAAE CSB-MP760774BAAE CSB-EP760774BAAE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Bacillus thuringiensis subsp. konkukian 50S ribosomal protein L18 (rplR) | CSB-YP760775BAAE CSB-EP760775BAAE CSB-BP760775BAAE CSB-MP760775BAAE CSB-EP760775BAAE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Bacillus thuringiensis subsp. konkukian 50S ribosomal protein L14 (rplN) | CSB-YP760776BAAE CSB-EP760776BAAE CSB-BP760776BAAE CSB-MP760776BAAE CSB-EP760776BAAE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Bacillus thuringiensis subsp. konkukian 30S ribosomal protein S19 (rpsS) | CSB-YP760777BAAE CSB-EP760777BAAE CSB-BP760777BAAE CSB-MP760777BAAE CSB-EP760777BAAE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Bacillus thuringiensis subsp. konkukian DNA-directed RNA polymerase subunit beta (rpoB), partial | CSB-YP760778BAAE CSB-EP760778BAAE CSB-BP760778BAAE CSB-MP760778BAAE CSB-EP760778BAAE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Bacillus thuringiensis subsp. konkukian 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) | CSB-YP760779BAAE CSB-EP760779BAAE CSB-BP760779BAAE CSB-MP760779BAAE CSB-EP760779BAAE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Bacillus thuringiensis subsp. konkukian Putative septation protein spoVG (spoVG) | CSB-YP760780BAAE CSB-EP760780BAAE CSB-BP760780BAAE CSB-MP760780BAAE CSB-EP760780BAAE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Bacillus thuringiensis subsp. konkukian Chromosomal replication initiator protein DnaA (dnaA) | CSB-YP760781BAAE CSB-EP760781BAAE CSB-BP760781BAAE CSB-MP760781BAAE CSB-EP760781BAAE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Putative adenine deaminase BA_3032/GBAA_3032/BAS2818 (BA_3032, GBAA_3032, BAS2818), partial | CSB-YP760782BQE CSB-EP760782BQE CSB-BP760782BQE CSB-MP760782BQE CSB-EP760782BQE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Oryza sativa subsp. japonica Germin-like protein 5-1 (Os05g0277500, LOC_Os05g19670) | CSB-YP760783OFG CSB-EP760783OFG CSB-BP760783OFG CSB-MP760783OFG CSB-EP760783OFG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Influenza C virus Polyprotein p42 (M), partial | CSB-YP760784IKB CSB-EP760784IKB CSB-BP760784IKB CSB-MP760784IKB CSB-EP760784IKB-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Human Dehydrogenase/reductase SDR family member 7B (DHRS7B), partial | CSB-YP760785HU CSB-EP760785HU CSB-BP760785HU CSB-MP760785HU CSB-EP760785HU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Human Protein LDOC1L (LDOC1L) | CSB-YP760786HU CSB-EP760786HU CSB-BP760786HU CSB-MP760786HU CSB-EP760786HU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Human Aspartate beta-hydroxylase domain-containing protein 2 (ASPHD2), partial | CSB-YP760787HU CSB-EP760787HU CSB-BP760787HU CSB-MP760787HU CSB-EP760787HU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Arabidopsis thaliana Metal tolerance protein C2 (MTPC2), partial | CSB-YP760788DOA1 CSB-EP760788DOA1 CSB-BP760788DOA1 CSB-MP760788DOA1 CSB-EP760788DOA1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase makorin (MKRN) | CSB-YP760789DOA CSB-EP760789DOA CSB-BP760789DOA CSB-MP760789DOA CSB-EP760789DOA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Mouse Serine protease inhibitor Kazal-type 7 (Spink7) | CSB-YP760790MO CSB-EP760790MO CSB-BP760790MO CSB-MP760790MO CSB-EP760790MO-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Human Serine protease inhibitor Kazal-type 14 (SPINK14) | CSB-YP760791HU CSB-EP760791HU CSB-BP760791HU CSB-MP760791HU CSB-EP760791HU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
Recombinant Rat Serine protease 57 (Prss57) | CSB-YP760792RA CSB-EP760792RA CSB-BP760792RA CSB-MP760792RA CSB-EP760792RA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
有用資源
什么是重組蛋白?
重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。
第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。
第二條:如何選擇合適的表達(dá)載體?
表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。
根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://www.artbeat.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。
引用資料
Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023
Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023
Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023
Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023
Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022