Recombinant Rat Potassium channel subfamily K member 10 (Kcnk10)

in vitro E.coli expression system

中文名稱：

大鼠Kcnk10重組蛋白
貨號(hào)：

CSB-CF862371RA
說(shuō)明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

in vitro E.coli expression system
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

基因名：

Kcnk10
Uniprot No.：

Q9JIS4
別名：

Kcnk10; Trek2; Potassium channel subfamily K member 10; Outward rectifying potassium channel protein TREK-2; TREK-2 K(+ channel subunit
種屬：

Rattus norvegicus (Rat)
蛋白長(zhǎng)度：

full length protein
表達(dá)區(qū)域：

1-538
氨基酸序列

MKFPIETPRKQVNWDPKVAVPAAAPPVCQPKSATNGHHPVPRLSISSRATVVARMEGASQ GGLQTVMKWKTVVAIFVVVVVYLVTGGLVFRALEQPFESSQKNTIALEKAEFLRDHICVS PQELETLIQHALDADNAGVSPVGNSSNSSSHWDLGSAFFFAGTVITTIGYGNIAPSTEGG KIFCILYAIFGIPLFGFLLAGIGDQLGTIFGKSIARVEKVFRKKQVSQTKIRVISTILFI LAGCIVFVTIPAVIFKYIEGWTALESIYFVVVTLTTVGFGDFVAGGNAGINYREWYKPLV WFWILVGLAYFAAVLSMIGDWLRVLSKKTKEEVGEIKAHAAEWKANVTAEFRETRRRLSV EIHDKLQRAATIRSMERRRLGLDQRAHSLDMLSPEKRSVFAALDTGRFKASSQESINNRP NNLRLKGPEQLNKHGQGASEDNIINKFGSTSKLTKRKNKDLKKTLPEDVQKIYKTFRNYS LDEEKKEDETEKMCNSDNSSTAMLTECIQQQAEMENGMVPMDTKDQGLENNSLLEDRN
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 10xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Outward rectifying potassium channel. Produces rapidly activating and non-inactivating outward rectifier K(+) currents. Activated by arachidonic acid and other naturally occurring unsaturated free fatty acids.
基因功能參考文獻(xiàn)：
1. strong immunoreactivity of TRAAK, TREK-1 and TREK-2 channels was present mainly in small-sized dorsal root ganglion (DRG) neurons of rats. PMID: 29980241
2. Data do not suggest a relevant impact of miRNA expression patterns on the pharmacoresponsiveness of temporal lobe epilepsy; but confirmed the regulation of the previously described miRNA-187-3p and demonstrated that its regulation can exert an impact on the expression of the two-pore domain potassium channel protein KCNK10/TREK-2. PMID: 27609046
3. Up-regulation of TREK-2 potassium channels in cultured astrocytes requires de novo protein synthesis.TREK-2 channels are up-regulated in the astrocytic membrane during ischemia. PMID: 25886567
4. KCNK10 contributes to the regulation of MCE through the control of C/EBPbeta and C/EBPdelta expression and insulin signaling. PMID: 25501330
5. We conclude that TREK2 hyperpolarizes IB4 binding C-nociceptors and limits pathological spontaneous pain. PMID: 24453337
6. In the dorsal horn of the developing rat, KCNK10 mRNA was decreased in mature compared with developing rats. PMID: 23219908
7. These results suggest that baicalein- and wogonin-induced TREK-2 activation help set the resting membrane potential of cells exposed to pathological conditions and thus may give beneficial effects in neuroprotection. PMID: 21306568
8. is functionally expressed in cortical astrocytes in culture, and may be involved in K+ homeostasis of astrocytes during pathological states PMID: 11897089
9. TREK-2 is a temperature-sensitive channel active at physiological body temperature, and therefore would contribute to the background K+ conductance and regulate cell excitability in response to various physical and chemical stimuli. PMID: 15677687
10. Agonist-induced inhibition of TREK-2 via muscarinic M(3) receptor occurs primarily via PKC-mediated phosphorylation. PMID: 16672694
11. TREK-2 exhibits two primary modes of gating that give rise to two channel phenotypes under dephosphorylated conditions and its phosphorylation shifts the gating mode to include intermediate conducting levels. PMID: 17540699
12. The small and large conductance TREK-2 channels are produced as a result of alternative translation initiation, producing isoforms with long and short N-termini. The distal half of the N-terminus controls the unitary conductance. PMID: 18845607
13. Results indicate a novel action mode in which norepinephrine depresses neuronal excitability in the entorhinal cortex by disinhibiting protein kinase A-mediated tonic inhibition of TREK-2 channels. PMID: 19244246
14. present results revealed that TREK-1, TREK-2 and TRAAK channels colocalized with thermosensitive TRP channels in some small trigeminal ganglion neurons PMID: 19429069
15. Compared the properties of cloned TREK-2 with large-conductance background K+ channels. PMID: 19439530
16. GABA(B) receptor-mediated inhibition in the EC was mediated via activating TREK-2, a type of two-pore domain K(+) channels, and required the functions of inhibitory G proteins and protein kinase A pathway. PMID: 19640481
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亞細(xì)胞定位：

Membrane; Multi-pass membrane protein.
蛋白家族：

Two pore domain potassium channel (TC 1.A.1.8) family
組織特異性：

Expressed mainly in the cerebellum, spleen, and testis.
數(shù)據(jù)庫(kù)鏈接：

KEGG: rno:65272

STRING: 10116.ENSRNOP00000005175

UniGene: Rn.53999