Recombinant Rat Claudin-5 (Cldn5)-VLPs

中文名稱：

Recombinant Rat Claudin-5 (Cldn5)-VLPs
貨號(hào)：

CSB-MP867513RA
說(shuō)明書：

MSDS Datasheet
規(guī)格：
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

基因名：

Cldn5
Uniprot No.：

Q9JKD6
種屬：

Rattus norvegicus (Rat)
蛋白長(zhǎng)度：

Full Length
來(lái)源：

Mammalian cell
表達(dá)區(qū)域：

1-218aa
氨基酸序列

MGSAALEILGLVLCLVGWVGLILACGLPMWQVTAFLDHNIVTAQTTWKGLWMSCVVQSTGHMQCKVYESVLALSAEVQAARALTVGAVLLALVALFVTLTGAQCTTCVAPGPVKARVALTGGALYALCGLLALVPLCWFANIVVREFYDPTVPVSQKYELGAALYIGWAASALLMCGGGLVCCGAWVCTGRPEFSFPVKYSAPRRTTANGDYDKKNYV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

C-terminal 10xHis-tagged
If you have specified tag type, please tell us and we will check if it's possible to develop.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from PBS, 6% Trehalose, pH 7.4.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL.Aliquot for long-term storage at -80℃. Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項(xiàng)：

The VLPs are expressed from human 293 cells (HEK293).Mix the sample gently by repeatedly pipetting it up and down. Do not vortex. Repeated freezing and thawing is not recommended.Store the protein at -20℃/-80℃ upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity. The immunization strategy should be optimized (antigen dose, regimen and adjuvant).
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Plays a major role in tight junction-specific obliteration of the intercellular space.
基因功能參考文獻(xiàn)：
1. claudin-5 might represent a novel pharmacological target for treating acute lung injury induced by H2S and other hazardous gases. PMID: 28741371
2. in response to alcohol, increased claudin-5 paradoxically accompanies an increase in paracellular leak and rearrangement of alveolar tight junctions. PMID: 27452368
3. Immunocytochemistry and Western blotting showed that claudin-5, a critical regulatory protein for tight junctions, was translocated from the membrane to the cytoplasm after cooling in cultured bEnd3 cells PMID: 23653089
4. Increased claudin-5 and claudin-1 expression and decreased permeability of the blood brain barrier were observed in the hyperbaric oxygenation and cerebral ischemia-reperfusion injury group. PMID: 21626096
5. Claudin-5 redistribution, mediated by caveolin-1, was observed in blood brain barrier following ischemic stroke. PMID: 22378877
6. Levels of tight junction protein claudin 5 are decreased in blood-brain barrier cells post-translationally following neurotoxicant administration. PMID: 20970449
7. Blood-brain barrier permeability increases after stroke through matrix metalloprotein-mediated degradation of claudin-5 and occuludin in tight junctions. PMID: 21717368
8. Data demonstrate the timing of tight junction-associated proteins claudin-5, occludin, and ZO-1 in light of blood-brain barrier permeability associated with cerebral ischemia reperfusion. PMID: 21318404
9. These findings suggest that claudin-5 is a main claudin expressed in podocytes and that the formation of tight junctions in the nephrosis may be due to local recruitment of claudin-5 rather than due to total upregulation of the claudin protein levels. PMID: 21271259
10. claudin-5 and occludin translocate reversibly within cells of the rat blood-optic nerve barrier after borneol treatment PMID: 20473716
11. Increased buffer concentration by addition of HEPES, MOPS, or TES to the media during differentiation increased independent of the type of buffer. This correlated with increased expression of claudin-5 PMID: 20967520
12. MUPP1 and claudin-5 colocalized in the incisures, and the COOH-terminal region of claudin-5 interacts with MUPP1 in a PSD-95/Disc Large/zona occludens (ZO)-1 (PDZ)-dependent manner in tight junctions PMID: 12403818
13. Induction of wild-type claudin-5 is sufficient to reconstitute the paracellular barrier against inulin (5 kDa), but not mannitol (182 Da), in leaky rat lung epithelial cells PMID: 15383327
14. Data show that adrenomedullin inhibits paracellular transport in a blood-brain barrier in vitro model through claudin-5 overexpression. PMID: 16763778
15. Claudin 5 is predominantly expressed in brain capillaries, and plays a key role in the appearance of barrier properties of brain capillary endothelial cells. PMID: 16998798
16. Immunogold electron microscopy demonstrated claudin-5 localized in the tight-junctional fused membranes of adjacent endothelial cells. PMID: 17899156
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亞細(xì)胞定位：

Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.
蛋白家族：

Claudin family
數(shù)據(jù)庫(kù)鏈接：

KEGG: rno:65131

STRING: 10116.ENSRNOP00000064048

UniGene: Rn.17420