Recombinant Mouse TYRO protein tyrosine kinase-binding protein (Tyrobp)

in vitro E.coli expression system

中文名稱：

小鼠Tyrobp重組蛋白
貨號：

CSB-CF025398MO
說明書：

MSDS Datasheet
規格：
來源：

in vitro E.coli expression system
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

基因名：

Tyrobp
Uniprot No.：

O54885
別名：

Tyrobp; Dap12; Karap; TYRO protein tyrosine kinase-binding protein; DNAX-activation protein 12; Killer-activating receptor-associated protein; KAR-associated protein
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length of Mature Protein
表達區域：

22-114
氨基酸序列

LSPVQAQSDTFPRCDCSSVSPGVLAGIVLGDLVLTLLIALAVYSLGRLVSRGQGTAEGTRKQHIAETESPYQELQGQRPEVYSDLNTQRQYYR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Adapter protein which non-covalently associates with activating receptors found on the surface of a variety of immune cells to mediate signaling and cell activation following ligand binding by the receptors. TYROBP is tyrosine-phosphorylated in the ITAM domain following ligand binding by the associated receptors which leads to activation of additional tyrosine kinases and subsequent cell activation. Also has an inhibitory role in some cells. Non-covalently associates with activating receptors of the CD300 family to mediate cell activation. Also mediates cell activation through association with activating receptors of the CD200R family. Required for neutrophil activation mediated by integrin. Required for the activation of myeloid cells mediated by the CLEC5A/MDL1 receptor. Associates with natural killer (NK) cell receptors such as the KLRD1/KLRC2 heterodimer to mediate NK cell activation. Also associates non-covalently with the NK cell receptors KLRA4/LY49D and KLRA8/LY49H which leads to NK cell activation. Associates with TREM1 to mediate activation of neutrophils and monocytes. Associates with TREM2 on monocyte-derived dendritic cells to mediate up-regulation of chemokine receptor CCR7 and dendritic cell maturation and survival. Association with TREM2 mediates cytokine-induced formation of multinucleated giant cells which are formed by the fusion of macrophages. Stabilizes the TREM2 C-terminal fragment (TREM2-CTF) which is produced by TREM2 ectodomain shedding. In microglia, required with TREM2 for phagocytosis of apoptotic neurons. Required with ITGAM/CD11B in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development. Promotes proinflammatory responses in microglia following nerve injury which accelerates degeneration of injured neurons. Positively regulates the expression of the IRAK3/IRAK-M kinase and IL10 production by liver dendritic cells and inhibits their T cell allostimulatory ability. Negatively regulates B cell proliferation. Required for CSF1-mediated osteoclast cytoskeletal organization. Positively regulates multinucleation during osteoclast development.
基因功能參考文獻：
1. We identified modulatory effects of TYROBP deficiency on the level of phosphorylation of TAU that was accompanied by a reduction in the severity of neuritic dystrophy. TYROBP deficiency also altered the expression of several AD related genes, including Cd33. Electrophysiological abnormalities and learning behavior deficits associated with APP/PSEN1 transgenes were greatly attenuated on a Tyrobp-null background. PMID: 28612290
2. These results demonstrate that PTX targets the innate immunity through DAP12, FcRgamma, and MyD88 providing new insights into the immunobiology of PTX. PMID: 28089363
3. Recent studies have revealed that activated microglia in the spinal dorsal horn exacerbate neuropathic pain, which has suggested that suppression of microglial activity should be considered as a therapeutic target. However, only a few molecules have been identified as regulators of microglial activity. In this study, we focused on a receptor complex of TREM2 and DAP12, both of which are expressed by microglia and have bee PMID: 27798193
4. TNF/IL-6 generated osteoclasts in vitro that resorbed mineralized tissue through a pathway dependent on IL-6R, NFATc1, DNAX-activation protein 12, and cell proliferation. PMID: 27563728
5. The findings suggest that DAP12 acts as a brake on the pulmonary immune response to C. neoformans by promoting pulmonary eosinophilia and by inhibiting the activation and antifungal activities of effector cells, including NK cells and macrophages. PMID: 27068093
6. significant role of DAP12 in the initial inflammatory response, bone remodeling and regeneration PMID: 26030755
7. DAP12-mediated microglial activation following axotomy promotes pro-inflammatory responses, and thereby accelerates nerve injury-induced neuron death PMID: 25690660
8. results support a role of DAP12 in stabilizing TREM2-CTF, thereby protecting against excessive pro-inflammatory responses. PMID: 25957402
9. a previously unrecognized role for DAP12 expression in tissue-resident alveolar macrophages in mediating acute noninfectious tissue injury through regulation of neutrophil trafficking. PMID: 25762783
10. DAP12 expression in liver grafts regulates donor mDC migration to host lymphoid tissue, alloreactive T cell responses and transplant tolerance PMID: 24935196
11. Integrin alphav beta3/Dap12 double-deficient developed profound osteoporosis. PMID: 25547154
12. Our study identifies the DAP12/IRAK-M/IL-10 to be a novel molecular pathway in APCs exploited by mycobacterial pathogens, allowing infection a foothold in the lung. PMID: 24172845
13. We conclude that DAP12, partly through TREM1/3, is involved in renal inflammation during progression of unilateral ureter obstruction. PMID: 24358193
14. Siglec-15 regulates osteoclast development and bone resorption by modulating receptor activator of nuclear factor kappaB ligand (RANKL) signaling in association with DAP12. PMID: 23677868
15. Dap12- and FcRgamma-deficiency exacerbates Granulocyte-Macrophage Colony-Stimulating Factor-driven monocyte differentiation and production of inflammatory monocyte-derived dendritic cells. PMID: 24155889
16. The effects of DAP12 deficiency on development of autoimmune uveitis are dramatically affected by environmental factors. PMID: 23906311
17. DOK3 physically associated with the ITAM of DAP12 through its phosphotyrosine-binding domain. In response to LPS, DOK3 was phosphorylated in a DAP12- and Src-dependent manner, which led to translocation of phosphorylated DOK3 to the plasma membrane. PMID: 23962980
18. DAP12 is involved in microglia-induced dopaminergic neuron cell death in an in vitro model of Parkinson's disease. PMID: 23844828
19. the Siglec-15-DAP12-Syk-signaling cascade plays a critical role in functional osteoclast formation. PMID: 22451653
20. Because mouse and rat NKG2C and -E transmembrane regions contain a positively charged arginine residue located close to the extracellular surface, these receptors may not associate with DAP12. PMID: 22084441
21. we report that the emergence of immunopathogenic CD4 T cells is under the control of a transmembrane immunoadaptor DAP12 pathway during influenza infection. PMID: 21908731
22. Data suggest that OSCAR is a collagen receptor that binds to specific collagen motifs and costimulates osteoclastogenesis in DAP12-deficient humans and mice. PMID: 21841309
23. DAP12-coupled MAIR-II negatively regulates B cell-mediated adaptive immune responses. PMID: 21727189
24. A regulatory role is highlighted for DAP12 in hepatic dendritic cell maturation; a mechanism via interleukin-1 receptor-associated kinase 3 (IRAK-M) is auggested whereby this function may be induced and maintained. PMID: 21257958
25. Cytoskeletal dysfunction, and not arrested differentiation, is the dominant consequence of DAP12 deficiency in osteoclasts. PMID: 20720152
26. demonstrate a previously uncharacterized interaction of SHIP1 with DAP12 that limits TREM2- and DAP12-dependent signaling and identify a mechanism through which SHIP1 regulates key ITAM-containing receptors by blocking the binding and activation of PI3K PMID: 20484116
27. These findings indicate that DAP12, possibly through association with TREM2, contributes to alveolar macrophage chemotaxis and recruitment to the lung and may mediate macrophage accumulation in lung diseases such as emphysema. PMID: 20421649
28. DAP12-associating lectin (MDL)-1 receptor is a key regulator of synovial injury and bone erosion in autoimmune joint inflammation. PMID: 20212065
29. DAP12 and mature lymphocytes act synergistically to maintain normal bone mass, while playing similar but not synergistic roles in protecting bone loss after gonadal failure. PMID: 17611620
30. a crucial role for a particular activating signaling pathway, in this case the one induced through KARAP/DAP12, in the NK cell-mediated resistance to an infection PMID: 11927627
31. Aberrant DAP12 signaling in the 129 strain of mice: implications for the analysis of gene-targeted mice. PMID: 12165492
32. selective associations with DAP12 determine stimulatory versus costimulatory activity of NKG2D PMID: 12426565
33. DAP12 has a role in signal transduction, bone modeling, and brain myelination [review] PMID: 12569153
34. DAP12 has roles in preventing synaptic degeneration, impaired prepulse inhibition, and aberrant electrophysiological profiles in the thalami PMID: 12569157
35. stimulation of the DAP12 adapter protein plays a significant role in formation of multinuclear osteoclasts and that DAP12 and DARs likely participate in the regulation of bony remodeling PMID: 14969392
36. DAP12 and FcRgamma have overlapping roles in supporting osteoclast development in osteoblast-osteoclast cocultures, which mirrors their overlapping functions in vivo PMID: 15073337
37. DAP12 and its associating molecules are differentially regulated by mycobacterial infection and type 1 cytokines PMID: 15102754
38. IL-4-mediated dendritic cell (DC)-activated natural killer (NK) signaling is suppressed in vitro by KARAP/DAP12. PMID: 15128777
39. KARAP/DAP12 may alter microglial physiology and subsequently synaptic function and plasticity through a novel microglia-neuron interaction. PMID: 15601948
40. KARAP/DAP12-mediated signals play an important role in the regulation of both brain and bone homeostasis as seen in loss-of function mice. PMID: 15632019
41. The importance of Fcgr3/Cd3z, Hcst, and Tyrobp in the activation of NK cells in the uterus of pregnant mice is reported. PMID: 15878887
42. one or more DAP12-pairing receptors negatively regulate signaling through TLRs PMID: 15895090
43. DAP12 has a role in mediating effector function in an NKG2D-dependent and major histocompatibility complex-independent manner PMID: 16169855
44. Consistent and selective association of the IDO-competent phenotype with down-modulation of the Tyrobp gene. PMID: 16339401
45. plexin-A1 associates with the triggering receptor expressed on myeloid cells-2 (Trem-2), linking semaphorin-signalling to the immuno-receptor tyrosine-based activation motif (ITAM)-bearing adaptor protein, DAP12 PMID: 16715077
46. DAP12 must associate with a receptor present in the macrophage to inhibit Toll-like receptor- and Fc receptor-induced tumor necrosis factor (TNF) production. PMID: 16887962
47. DAP12 signaling in plasmacytoid dendritic cells (pDC) can mediate different regulatory effects on their functions, depending on the mechanisms of pDC activation PMID: 16920926
48. Ly49H signaling through DAP12 appears to directly augment NK cell sensitivity to low concentrations of proproliferative cytokines such as IL-15. PMID: 17015680
49. This review discusses the dual functionality of DAP12 and presents evidence that DAP12 can suppress as well as activate natural killer (NK) cells. PMID: 17100880
50. FcRgamma chain may provide cooperation with and/or compensation for DAP12 in MAIR-II-mediated inflammatory responses by peritoneal macrophages PMID: 17202337
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亞細胞定位：

Cell membrane; Single-pass type I membrane protein.
蛋白家族：

TYROBP family
組織特異性：

Expressed on microglia (at protein level). Expressed on oligodendrocytes (at protein level). Expressed on macrophages and osteoclasts. Expressed on dendritic cells in liver, spleen, kidney and lung with highest levels in liver dendritic cells.
數據庫鏈接：

KEGG: mmu:22177

STRING: 10090.ENSMUSP00000032800

UniGene: Mm.46301