Recombinant Mouse Synaptotagmin-2 (Syt2)

in vitro E.coli expression system

中文名稱：

小鼠Syt2重組蛋白
貨號：

CSB-CF023038MO
說明書：

MSDS Datasheet
規格：
來源：

in vitro E.coli expression system
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

基因名：

Syt2
Uniprot No.：

P46097
別名：

Syt2; Synaptotagmin-2; Inositol polyphosphate-binding protein; IP4-binding protein; IP4BP; Synaptotagmin II; SytII
種屬：

Mus musculus (Mouse)
蛋白長度：

full length protein
表達區域：

1-422
氨基酸序列

MRNIFKRNQEPNVAPATTTATMPLAPVAPADNSTESTGPGESQEDMFAKLKEKFFNEINKIPLPPWALIAMAVVAGLLLLTCCFCICKKCCCKKKKNKKEKGKGMKNAMNMKDMKGGQDDDDAETGLTEGEGEGEEEKEPENLGKLQFSLDYDFQANQLTVGVLQAAELPALDMGGTSDPYVKVFLLPDKKKKYETKVHRKTLNPAFNETFTFKVPYQELAGKTLVMAIYDFDRFSKHDIIGEVKVPMNTVDLGQPIEEWRDLQGGEKEEPEKLGDICTSLRYVPTAGKLTVCILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKKKTTVKKKTLNPYFNESFSFEIPFEQIQKVQVVVTVLDYDKLGKNEAIGKIFVGSNATGTELRHWSDMLANPRRPIAQWHSLKPEEEVDALLGKNK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Exhibits calcium-dependent phospholipid and inositol polyphosphate binding properties. May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. Plays a role in dendrite formation by melanocytes.; (Microbial infection) Receptor for C.botulinum neurotoxin type B (BoNT/B, botB); interaction is improved in the presence of gangliosides. The toxin binds via the vesicular domain (residues 47-60).; (Microbial infection) Receptor for C.botulinum neurotoxin type G (BoNT/G, botG); gangliosides are not required for (or only very slightly improve) binding to a membrane-anchored receptor fragment. The toxin binds via the vesicular domain (residues 47-55).
基因功能參考文獻：
1. Authors propose that the strong reduction of Syt2 and SV2B are key factors of the functional synaptic alteration and that the physiological downregulation of Syt1 plays a determinant role in muscle vulnerability in SMA PMID: 28173138
2. Deletion of Syt2 severely reduced and delayed disynaptic inhibition following parallel fiber stimulation and as release sensor at GABAergic basket cell-Purkinje cell synapses ensures fast and efficient feedforward inhibition in cerebellar microcircuit. PMID: 28099850
3. aM inhibitors or the inhibition of the Ca(2+)-calmodulin-Munc13-1 signaling pathway only impaired the uptake of Syt2 while leaving membrane retrieval intact, indicating different recycling mechanisms for membranes and vesicle proteins. Our data identify a novel mechanism of stimulus- and Ca(2+)-dependent regulation of coordinated endocytosis of synaptic membranes and vesicle proteins. PMID: 27154627
4. The 2.3-A structure of a ternary complex of botulinum neurotoxin type B bound to both its protein receptor synaptotagmin II and its ganglioside receptor GD1a, is reported. PMID: 23807078
5. Syt2 make it an excellent marker for analyzing the development and plasticity of perisomatic inhibitory innervations onto both excitatory and inhibitory neurons in the visual cortex. PMID: 22539967
6. Syt2 increases the dynamic range of synapses by driving release with a high Ca(2)+ cooperativity, as well as by suppressing a remaining, near-linear Ca(2)+ sensor. PMID: 21338883
7. Transient expression of Syt2 is no longer detected in cochlear inner hair cells (IHCs) after the onset of hearing, indicating that the most common calcium-sensors in central nervous system synapses are not involved in mature IHC ribbon synapse. PMID: 20926654
8. SYT2 was a negative regulator of chemotaxis PMID: 20473299
9. Synaptotagmins I and II act as nerve cell receptors for botulinum neurotoxin G PMID: 15123599
10. The readily releasable pool was rescued by Syt2 overexpression. The kinetics of fusion was slightly slower than in cells expressing Syt1. Syt2 has a lower Ca2+ affinity for phospholipid binding than Syt1 because of a difference in the C2A domain. PMID: 16407561
11. Data suggest that synaptotagmins 1 and 2 perform equivalent functions in the Ca2+ triggering of action potential-induced neurotransmitter release and in the restriction of spontaneous release. PMID: 16642042
12. Synaptotagmin-2 is an essential synaptotagmin isoform that functions in concert with other synaptotagmins in the calcium-ion triggering of neurotransmitter release. PMID: 17192432
13. Data show that lack of Synaptotagmin-2 in mast cells was not associated with increased spontaneous exocytosis. PMID: 19473977
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亞細胞定位：

Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass membrane protein. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane; Single-pass membrane protein. Cytoplasm.
蛋白家族：

Synaptotagmin family
數據庫鏈接：

KEGG: mmu:20980

STRING: 10090.ENSMUSP00000112438

UniGene: Mm.5102