Recombinant Mouse Potassium voltage-gated channel subfamily KQT member 1 (Kcnq1)

in vitro E.coli expression system

中文名稱：

小鼠Kcnq1重組蛋白
貨號：

CSB-CF012087MO
說明書：

MSDS Datasheet
規格：
來源：

in vitro E.coli expression system
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

基因名：

Kcnq1
Uniprot No.：

P97414
別名：

Kcnq1; Kcna9; Kvlqt1; Potassium voltage-gated channel subfamily KQT member 1; IKs producing slow voltage-gated potassium channel subunit alpha KvLQT1; KQT-like 1; Voltage-gated potassium channel subunit Kv7.1
種屬：

Mus musculus (Mouse)
蛋白長度：

Full length protein
表達區域：

1-668
氨基酸序列

MDTASSPPSAERKRAGWSRLLGARRGSAVVKKCPFSLELAEGGPEGSTVYAPIAPTGAPG LAPPMSTPVSPAPAPADLGPRPRVSLDPRVSIYSARRPLLARTHIQGRVYNFLERPTGWK CFVYHFTVFLIVLVCLIFSVLSTIEQYAALATGTLFWMEIVLVVFFGTEYVVRLWSAGCR SKYVGIWGRLRFARKPISIIDLIVVVASMVVLCVGSKGQVFATSAIRGIRFLQILRMLHV DRQGGTWRLLGSVVFIHRQELITTLYIGFLGLIFSSYFVYLAEKDAVNESGRIEFGSYAD ALWWGVVTVTTIGYGDKVPQTWVGKTIASCFSVFAISFFALPAGILGSGFALKVQQKQRQ KHFNRQIPAAASLIQTAWRCYAAENPDSATWKIYVRKPARSHTLLSPSPKPKKSVMVKKK KFKLDKDNGMSPGEKMFNVPHITYDPPEDRRPDHFSIDGYDSSVRKSPTLLEVSTPHFLR TNSFAEDLDLEGETLLTPITHVSQLRDHHRATIKVIRRMQYFVAKKKFQQARKPYDVRDV IEQYSQGHLNLMVRIKELQRRLDQSIGKPSLFIPISEKSKDRGSNTIGARLNRVEDKVTQ LDQRLVIITDMLHQLLSMQQGGPTCNSRSQVVASNEGGSINPELFLPSNSLPTYEQLTVP QTGPDEGS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Potassium channel that plays an important role in a number of tissues, including heart, inner ear, stomach and colon. Associates with KCNE beta subunits that modulates current kinetics. Induces a voltage-dependent by rapidly activating and slowly deactivating potassium-selective outward current. Promotes also a delayed voltage activated potassium current showing outward rectification characteristic. During beta-adrenergic receptor stimulation participates in cardiac repolarization by associating with KCNE1 to form the I(Ks) cardiac potassium current that increases the amplitude and slows down the activation kinetics of outward potassium current I(Ks). Muscarinic agonist oxotremorine-M strongly suppresses KCNQ1/KCNE1 current. When associated with KCNE3, forms the potassium channel that is important for cyclic AMP-stimulated intestinal secretion of chloride ions. This interaction with KCNE3 is reduced by 17beta-estradiol, resulting in the reduction of currents. During conditions of increased substrate load, maintains the driving force for proximal tubular and intestinal sodium ions absorption, gastric acid secretion, and cAMP-induced jejunal chloride ions secretion. Allows the provision of potassium ions to the luminal membrane of the secretory canaliculus in the resting state as well as during stimulated acid secretion. When associated with KCNE2, forms an heterooligomer complex leading to currents with an apparently instantaneous activation, a rapid deactivation process and a linear current-voltage relationship and decreases the amplitude of the outward current. When associated with KCNE4, inhibits voltage-gated potassium channel activity. When associated with KCNE5, this complex only conducts current upon strong and continued depolarization. Also forms a heterotetramer with KCNQ5; has a voltage-gated potassium channel activity. Binds with phosphatidylinositol 4,5-bisphosphate.
基因功能參考文獻：
1. Our data indicate that mouse embryonic stem cells are induced into islet-like cells in vitro. The gene imprinting status of Kcnq1 and Cdkn1c may be changed in differentiated cells during the induction in vitro. PMID: 28926866
2. Collectively, the authors propose that Prmt1-dependent facilitation of KCNQ-phosphatidylinositol-4,5-bisphosphate interaction underlies the positive regulation of KCNQ activity by arginine methylation, which may serve as a key target for prevention of neuronal hyperexcitability and seizures. PMID: 27466704
3. we investigated the effects of KCNQ1 A340E, a loss-of-function mutant. J343 mice bearing KCNQ1 A340E demonstrated a much higher 24-h intake of electrolytes (potassium, sodium, and chloride). KCNQ1, therefore, is suggested to play a central role in electrolyte metabolism. KCNQ1 A340E, with the loss-of-function phenotype, may dysregulate electrolyte homeostasis PMID: 27525866
4. Loss of methylation at the Kcnq1 imprinted gDMD was strongly associated with trophoblast giant cell (TGC) expansion. PMID: 26241757
5. Data show that disruption of potassium voltage-gated channel, KQT-like subfamily Q, member1 (KCNQ1) results in increased expression of cyclin-dependent kinase inhibitor 1C (Cdkn1c) only when the mutation is on the paternal allele. PMID: 26100882
6. S3 mutations in KCNQ1 cause diverse kinetic defects in I(Ks), affecting opening and closing properties, and can account for LQT1 phenotypes. PMID: 25444851
7. Characterization of the imprinted Kcnq1 domain which contains a differentially methylated region in intron 11 of Kcnq1. PMID: 25539921
8. KCNQ1, KCNE2, and SMIT1 form reciprocally regulating complexes that affect neuronal excitability. PMID: 24595108
9. low expression of KCNQ1 expression was significantly associated with poor overall survival. PMID: 23975432
10. Which participates in the allelic repression of Kcnq1. PMID: 24395636
11. H(+)-K(+)-ATPase/KCNQ1 reside in independent intracytoplasmic membrane compartments, or membrane domains, and upon activation of parietal cells, both membrane proteins are transported, possibly via Rab11-positive recycling endosomes, to apical membranes. PMID: 23154976
12. our studies reveal regulatory mechanisms within the Kcnq1 imprinted domain that operate exclusively in the heart on Kcnq1 a gene crucial for heart development and function. PMID: 23028363
13. Expression of KCNQ1 and NKCC1 protein in the stria vascularis of C57BL/6J mice decreases with age. PMID: 21426709
14. We showed the disturbance of parietal cell differentiation and mucous neck-to-zymogenic cell lineage differentiation with enhanced expression of KCNQ1 in the parietal cells. PMID: 20437101
15. Data show that in early pregnant mouse myometrium, the relative abundance of mRNA expression was KCNQ3 > KCNQ4 > KCNQ5 > KCNQ1 > KCNQ2. PMID: 20132415
16. Kcnq1 is expressed in the cell membrane of thyroid follicular cells and plays a significant role in thyroid function. plasma concentrations of T3/T4 are lower in Kcnq1 knockout mice than in their wild-type littermates PMID: 20978783
17. Repression of the paternal allele of several imprinted genes (including Kcnq1) on distal chromosome 7 is regulated by a non-coding antisense transcript, Kcnq1ot1, which is paternally expressed. PMID: 17021040
18. KCNQ1 loss-of-function mutation impairs gastric acid secretion in mice. PMID: 19306073
19. Data suggest that because the abundance and subcellular localization of KCNQ1 was unchanged in kcne3(-/-) mice, the modification of biophysical properties of KCNQ1 by KCNE3 is essential for its role in intestinal and tracheal transport. PMID: 20051516
20. Loss of Igf2 imprinting in monoclonal mouse hepatic tumor cells is not associated with abnormal methylation patterns for the H19 and Igf2 differentially methylated regions. PMID: 12475990
21. KCNQ1 and beta2-ARs associate and have a role in modulating the function of IKs channels under conditions of increased beta2-AR expression PMID: 15272004
22. Kcnq1 mutant mice are a powerful new tool for investigating the connection between acid balance, Helicobacter infection and mucin disruption in the progression to gastric cancer. PMID: 15385447
23. NF-Y transcription factor as a crucial regulator of antisense promoter-mediated bidirectional silencing and the parent of origin-specific epigenetic marks at the Kcnq1 imprinting control region PMID: 15459184
24. Based upon previous studies and the present results, it is concluded that both hKCNE4 and mKCNE4 have a drastic inhibitory impact on both hKCNQ1 and mKCNQ1 currents. PMID: 15707997
25. Kcnq1 knockout mice show histopathology comparable to that reported in human temporal bone cases of Jervell and Lange-Nielsen syndrome and provide further evidence that KCNQ1 channel dysfunction can lead to congenital deafness in this syndrome. PMID: 15891643
26. luminal KCNQ1 serves to repolarize the proximal tubule and stabilize the driving force for Na+ reabsorption PMID: 16314573
27. KCNQ1 has cell-specific roles in renal ion transport and may participate in K(+) secretion and/or absorption PMID: 16896189
28. Murine blood vessels exhibit a distinctive expression profile of KCNQ1, KCNQ4, and KCNQ5, with 'neuronal' KCNQ4 dominating PMID: 17519950
29. These results demonstrate for the first time that Kcnq1 protein is expressed in adult mouse hearts where it contributes to a beta-adrenergic-induced component of I(SS) that does not require co-assembly with Kcne1. PMID: 17597584
30. The Kcnq1 mutation in vtg-2J mice alters various physiological functions in the cardiac, gastric and adrenocortical systems. PMID: 17660684
31. We conclude that fenofibrate inhibits intestinal cAMP-stimulated Cl(-) secretion through a nongenomic mechanism that involves a selective inhibition of basolateral KCNQ1/KCNE3 channel complexes. PMID: 17916649
32. These results suggest that although it is expressed in nearly all taste bud cells, the function of KCNQ1 is not required for gross taste bud development or peripheral taste transduction pathways. PMID: 19006182
33. The mutation of KCNQ1 potassium channel did not affect the channel kinetics, whereas the surface expression increased with increasing hydrophobicity of the middle amino aicd residue. PMID: 19041715
34. The effect of APC in the regulation of gastric acid secretion requires H+/K+ ATPase activity and is at least partially due to SGK1-dependent upregulation of KCNQ1. PMID: 19255508
35. The observations indicate that KCNQ1 is a novel molecule affecting insulin sensitivity of glucose metabolism. PMID: 19369585
36. A 166 kb region near the Kcnq1 transcriptionally imprinted domain showed high recombination activity. PMID: 19439080
37. KCNQ1 channel provides K(+) to the extracellular K(+) binding site of the H(+)/K(+)-ATPase during acid secretion, and no other gastric K(+) channel can substitute for this function. PMID: 19491250
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亞細胞定位：

Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane. Early endosome. Membrane raft. Endoplasmic reticulum. Basolateral cell membrane.
蛋白家族：

Potassium channel family, KQT (TC 1.A.1.15) subfamily, Kv7.1/KCNQ1 sub-subfamily
組織特異性：

Expressed in heart, kidney and salivary glands. Detected in the cochlea. Almost undetectable in brain, skeletal muscle and liver. Widely expressed in embryonic and neonatal tissues.
數據庫鏈接：

KEGG: mmu:16535

STRING: 10090.ENSMUSP00000009689

UniGene: Mm.439769