Recombinant Mouse Ectodysplasin-A (Eda)

in vitro E.coli expression system

中文名稱：

小鼠Eda重組蛋白
貨號：

CSB-CF007389MO
說明書：

MSDS Datasheet
規格：
來源：

in vitro E.coli expression system
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

基因名：

Eda
Uniprot No.：

O54693
別名：

Eda; Ed1; Ta; Ectodysplasin-A; EDA protein homolog; Tabby protein
種屬：

Mus musculus (Mouse)
蛋白長度：

full length protein
表達區域：

1-391
氨基酸序列

MGYPEVERREPLPAAAPRERGSQGCGCRGAPARAGEGNSCRLFLGFFGLSLALHLLTLCCYLELRSELRRERGTESRLGGPGAPGTSGTLSSPGSLDPVGPITRHLGQPSFQQQPLEPGEDPLPPDSQDRHQMALLNFFFPDEKAYSEEESRRVRRNKRSKSGEGADGPVKNKKKGKKAGPPGPNGPPGPPGPPGPQGPPGIPGIPGIPGTTVMGPPGPPGPPGPQGPPGLQGPSGAADKTGTRENQPAVVHLQGQGSAIQVKNDLSGGVLNDWSRITMNPKVFKLHPRSGELEVLVDGTYFIYSQVEVYYINFTDFASYEVVVDEKPFLQCTRSIETGKTNYNTCYTAGVCLLKARQKIAVKMVHADISINMSKHTTFFGAIRLGEAPAS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Cytokine which is involved in epithelial-mesenchymal signaling during morphogenesis of ectodermal organs. Functions as a ligand activating the DEATH-domain containing receptors EDAR and EDA2R. Isoform TAA binds only to the receptor EDAR, while isoform TA-A2 binds exclusively to the receptor EDA2R. May also play a role in cell adhesion.; Isoform TAA binds only to the receptor EDAR, while isoform TA-A2 binds exclusively to the receptor EDA2R.; Isoform TA-A2 binds exclusively to the receptor EDA2R.
基因功能參考文獻：
1. Eda initiates a signaling cascade and recruits a BAF complex to specific gene loci to facilitate transcription during organogenesis. PMID: 30037996
2. Gain- and loss-of-function approaches reveal that liver-derived EDA regulates systemic glucose metabolism, suggesting that EDA is a hepatokine that can contribute to impaired skeletal muscle insulin sensitivity in obesity. PMID: 29106399
3. This is the first analysis of the role of Eda in the root, showing a direct role for this pathway during postnatal mouse development, and it suggests that changes in proliferation and angle of HERS may underlie taurodontism in a range of syndromes. PMID: 28813629
4. Data suggest that corneal epithelial integrity is defective and the thickness is reduced in Eda mutant Tabby mice at early postnatal stage (4-week-old); corneal epithelial cell proliferation is decreased and epithelial wound healing is delayed in Tabby mice, whereas it is restored by exogenous Eda. Tabby mice are a model for X-linked hypohidrotic ectodermal dysplasia and can be treated with recombinant Eda. PMID: 28655773
5. Murine nasal submucosal glands express EDA during embryonic development. EDA signalling is essential for Lateral Nasal Gland and Medial Nasal Gland budding and ductal morphogenesis. PMID: 27590203
6. Mouse models with HED also carry Eda, Edar or Edaradd mutations and have defects that map to the same structures.We report that otitis media, rhinitis and nasopharyngitis occur at high frequency in Eda and Edar mutant mice and explore the pathogenic mechanisms related to glandular function, microbial and immune parameters in these lines PMID: 27378689
7. Wnt, Eda, and Shh have roles in touch dome Merkel cell development PMID: 27414798
8. Using an ex vivo culture system, we show that suppression of canonical Wnt signalling leads to a dose-dependent inhibition of supernumerary placodes in K14-Eda tissue explants. PMID: 26581094
9. Eda and activin A are upstream regulators of Foxi3 in skin appendage placodes PMID: 23441037
10. Ectodysplasin regulates activator-inhibitor balance in murine tooth development through Fgf20 signaling. PMID: 22833125
11. NF-kappaB downstream of the TNF-like ligand ectodysplasin (Eda) is identified as a unique regulator of embryonic and prepubertal ductal morphogenesis. PMID: 22451941
12. Data found that Eda regulates growth and branching of the SMG via transcription factor NF-kappaB in the epithelium, and that the hedgehog pathway is an important mediator of Eda/NF-kappaB. PMID: 21652647
13. Dkk4 affects an auxiliary pathway for Eda-independent development of secondary hair PMID: 20386733
14. Treatment of Eda-/- minor salivary gland explants with EDA A1 rescues minor salivary gland induction. PMID: 20969842
15. The ectodysplasin-A gene plays a role in normal bone development in mice. PMID: 11945012
16. Eda and Edar expression is confined to the ectoderm and occurs in a pattern that suggests a role of ectodysplasin/Edar signaling in the interactions between the ectodermal compartments and the formation and function of hair placodes. PMID: 11973284
17. Gene expression profiling data are consistent with the regulation of the NF-kappa B and JNK pathways by EDA PMID: 12095918
18. ectodysplasin-Edar signaling has several roles in ectodermal organ development controlling their initiation, as well as morphogenesis and differentiation. PMID: 12812793
19. Analysis of expression patterns of eda, edar and tnfrsf19 in mouse embryogenesis. PMID: 12972005
20. Eda-A1 is among the key regulators of pattern formation in the skin PMID: 14502575
21. Inducible Eda-A1 transgene mediates sebaceous gland hyperplasia and differential formation of two classes of mouse hair follicles. PMID: 14506134
22. crystal structures of EDA-A1 and EDA-A2 show splice variants with distinct receptor specificity PMID: 14656435
23. EDA-A2 transgenic mice exhibited multifocal myodegeneration; this phenotype was not observed in the absence of XEDAR PMID: 14749376
24. Promotes placodal cell fate during early development of ectodermal organs. PMID: 15371307
25. with increasing expression levels of this one gene, the number of cusps increases, cusp shapes and positions change, longitudinal crests form, and number of teeth increases PMID: 15538367
26. isoforms of EDA-A5 and A5',activated NF-kappaB through receptors EDAR and XEDAR PMID: 16423472
27. Data show that, as an ectodysplasin target, lymphotoxin-beta regulates hair phenotype in developing hair follicles. PMID: 16738056
28. EDA signaling has a role in skin appendage development [review] PMID: 17102627
29. Localization of Shh expression by Wnt and Eda affects axial polarity and shape of hairs. PMID: 17376426
30. By analogy to the role played by eda in other ectodermal derivatives, we propose that it might play a role in defining the pattern of the palatal rugae. PMID: 18028050
31. Microarray profiling of genes differentially regulated by short exposure to recombinant Eda-A1 in embryonic eda(-/-) skin explants, was performed to identify direct targets of ectodysplasin pathway. PMID: 18508042
32. EDA signaling is needed for the determination and development of various skin appendages at spatiotemporally restricted intervals. PMID: 18923450
33. The upper jaw is more sensitive than the lower jaw to the loss of eda function. Modifications in cusp number and the abnormal crown size of the teeth are clearly linked, and our results indicate a role of eda in cusp patterning. PMID: 19051250
34. results indicate that unknown mechanisms of the Eda pathway are implicated in tooth morphogenesis PMID: 19340299
35. Salivary gland branching morphogenesis: a quantitative systems analysis of the Eda protein PMID: 19500387
36. The collagen domain activates EDA1 by multimerization, whereas the proteoglycan-binding domain may restrict the distribution of endogeneous EDA1 in vivo. PMID: 19657145
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相關疾病：

Defects in Eda are the cause of the tabby phenotype in mice (the equivalent of anhidrotic ectodermal dysplasia in humans). The disease is characterized by sparse hair (atrichosis or hypotrichosis), abnormal or missing teeth and the inability to sweat due to the absence of sweat glands.
亞細胞定位：

Cell membrane; Single-pass type II membrane protein.; [Ectodysplasin-A, secreted form]: Secreted.
蛋白家族：

Tumor necrosis factor family
數據庫鏈接：

KEGG: mmu:13607

STRING: 10090.ENSMUSP00000109409

UniGene: Mm.328086