Recombinant Human Sphingosine 1-phosphate receptor 2 (S1PR2)

中文名稱：

Recombinant Human Sphingosine 1-phosphate receptor 2 (S1PR2)
品名簡稱：

Recombinant Human S1PR2 protein
貨號：

CSB-CF020651HU
說明書：

MSDS Datasheet
規格：

￥12960
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

S1PR2
Uniprot No.：

O95136
別名：

Endothelial differentiation G-protein coupled receptor 5;Sphingosine 1-phosphate receptor Edg-5
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

in vitro E.coli expression system
分子量：

40.4 kDa
表達區域：

1-353aa
氨基酸序列

MGSLYSEYLNPNKVQEHYNYTKETLETQETTSRQVASAFIVILCCAIVVENLLVLIAVARNSKFHSAMYLFLGNLAASDLLAGVAFVANTLLSGSVTLRLTPVQWFAREGSAFITLSASVFSLLAIAIERHVAIAKVKLYGSDKSCRMLLLIGASWLISLVLGGLPILGWNCLGHLEACSTVLPLYAKHYVLCVVTIFSIILLAIVALYVRIYCVVRSSHADMAAPQTLALLKTVTIVLGVFIVCWLPAFSILLLDYACPVHSCPILYKAHYFFAVSTLNSLLNPVIYTWRSRDLRREVLRPLQCWRPGVGVQGRRRGGTPGHHLLPLRSSSSLERGMHMPTSPTFLEGNTVV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

C-terminal 10xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effects on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis. Receptor for the chemokine-like protein FAM19A5. Mediates the inhibitory effect of FAM19A5 on vascular smooth muscle cell proliferation and migration.
基因功能參考文獻：
1. both SphK1 overexpression and S1P addition increased mTOR phosphorylation as shown by ELISA, while S1PR2 inhibition had the inverse effect. These data suggest that CerS6 and SphK1 regulate mTOR signaling in breast cancer cell proliferation. Moreover, mTOR activity can be regulated by the balance between S1P and C16ceramide, which is generated by CerS6. PMID: 30226616
2. Although extravillous trophoblasts express three S1P receptor isoforms, S1P predominantly signals through S1PR2/Galpha12/13 to activate Rho, and thereby acts as potent inhibitor of extravillous trophoblast migration. PMID: 29208234
3. SNPs within 0.1 Mb of the S1PR2 gene as well as within the gene itself were interrogated as a candidate gene association for hearing loss. For 1 kHz thresholds, the adjacent SNP rs74930654 showed the most significant association. For 4 kHz, the most significant association was with rs201930568. These findings suggest that variants affecting the S1PR2 gene do contribute to auditory thresholds in the UK population. PMID: 27383011
4. High S1PR2 expression is associated with anti-neutrophil cytoplasmic antibody-associated vasculitis. PMID: 28206609
5. CONCLUSION: MiR-126 down-regulated S1PR2 and then prevented the activation of PI3K/AKT signaling pathway, which ultimately could damage intestinal mucosal barrier function. PMID: 28302479
6. Data suggest that activation of SR-BI by APOAI down-regulates sphingosine 1-phosphate/S1PR2-mediated inflammation in vascular endothelial cells by activating the PI3K/Akt signaling pathway; oxidized-LDL does the opposite. (APOA1 = apolipoprotein A-I; SR-BI/SCARB1 = scavenger receptor class B type I; S1PR2 = sphingosine 1-phosphate receptor 2; PI3K = phosphatidylinositol 3-kinase; Akt = proto-oncogene c-akt) PMID: 28181168
7. S1PR2 mediates Rho activation in normal cells neighboring RasV12-transformed cells. PMID: 26631556
8. Sphingosine 1-phosphate-induced IL-8 gene expression is mainly regulated via S1PR(1), and its secretion is regulated through S1PR(2) receptor subtype. PMID: 26321412
9. S1PR2 is repressed by FOXP1 in activated B-cell and germinal center B-cell DLBCL cell lines with aberrantly high FOXP1 levels; S1PR2 expression is further inversely correlated with FOXP1 expression in 3 DLBCL patient cohorts. PMID: 26729899
10. LXR-alpha might downregulate S1PR2 expression via miR-130a-3p in quiescent HUVECs. Stimulation of TNF-alpha attenuates the activity of LXR-alpha and results in enhanced S1PR2 expression. PMID: 26669941
11. be detected in the human cerebrovascular endothelium PMID: 26243335
12. S1PR2 plays a critical role in TCA-induced COX-2 expression and CCA growth and may represent a novel therapeutic target for CCA. PMID: 26518876
13. both S1PR1 and S1PR2 play a pivotal role in hyperglycemia-induced EC dysfunction and endothelial injury by reducing and enhancing the production of oxidative stress. PMID: 25673082
14. AB1 displayed potency at least equivalent to JTE-013 in affecting signaling molecules downstream of S1P2. PMID: 26105954
15. Activation of S1PR2-calcium influx-RhoA/ROCK dominates the high-dose S1P-induced endothelial monolayer hyperpermeability response. PMID: 25557733
16. Data indicate that sphingosine 1-phosphate (S1P) and hepatocyte growth factor (HGF) induced transloaction of integrin beta4, S1P receptors S1PR2 and S1PR3 to endothelial cell membrane caveolin-enriched microdomains (CEMs). PMID: 24851274
17. The S1P2R specifically activates RhoC via G12/13 proteins and LARG. PMID: 23993968
18. Sphingosylphosphorylcholine stimulates alpha-SMA protein expression and human lung fibroblast mediated collagen gel contraction via S1P2 receptor. PMID: 24614064
19. Conjugated bile acids promote cholangiocarcinoma growth through S1PR2. PMID: 24700501
20. S1PR2 expression was increased in disease-susceptible regions of the CNS of female patients with multiple sclerosis compared with their male counterparts. PMID: 24812668
21. we provide evidences that S1PR1/3, but not S1PR2, negatively regulate the expression of collagen in hMSCs using cellular and molecular approaches PMID: 24038457
22. extracellular S1P induces COX-2 expression via activation of S1P2 and subsequent Gi and p42/p44 MAPK-dependent signaling in renal mesangial cells leading to enhanced PGE2 formation and cell migration that essentially requires COX-2. PMID: 24064301
23. Activation of the S1P2 receptor counteracts the detrimental phosphorylation of p38 MAPK by IL-1beta. PMID: 23666803
24. S1PR2 is a key regulator of the proinflammatory phenotype of the endothelium. PMID: 23723450
25. S1PR agonists are pro-fibrotic via S1P2R and S1P3R stimulation using Smad-independent pathways. PMID: 23589284
26. Sphingosine 1-phosphate (S1P) receptors 1 and 2 coordinately induce mesenchymal cell migration through S1P activation of complementary kinase pathways PMID: 23300082
27. identify the S1PR2 as the specific and necessary receptor to induce phosphorylation of ERM proteins and subsequent filopodia formation PMID: 23106337
28. S1P receptors S1P1,2,3 are expressed in human anaplastic thyroid cancer C643 and THJ-16T cells at both mRNA and protein levels PMID: 22889737
29. abdominal aortic aneurysms have down-regulation of the S1P2 protein with simultaneous up-regulation of the S1P3 protein, but not S1P1 PMID: 22547907
30. Inflammatory mediators lipopolysaccharide and TNF-alpha induce S1PR2 expression in endothelium, suggesting that S1PR2 up-regulation may be involved in LPS and TNF-alpha elicited endothelial barrier dysfunction. PMID: 22244964
31. SphK/S1P/S1PRs signaling axis plays an important role in liver fibrosis and is involved in the directed migration of hepatic myofibroblasts into the damaged areas. PMID: 21145832
32. S1P2, and not S1P1 or S1P3, receptor activation increases conventional outflow resistance in whole-eye perfusions. PMID: 21289286
33. S1PR2 receptors play a critical role in regulating human mast cell functions, including degranulation and cytokine and chemokine release PMID: 20194630
34. suppress rac protein, a Rho family G protein and cell motility PMID: 11915348
35. Amyloid beta-protein stimulated in monocytes the gene expression for sphingosine-1-phosphate receptor 5, which is amyloid beta-protein-induced migration. PMID: 15208267
36. S1P2R receptor actively regulates the PTEN phosphatase by a Rho GTPase-dependent pathway to inhibit cell migration. PMID: 15764699
37. S1P2R activation in endothelial cells increases vascular permeability. The balance of S1P1 and S1P2 receptors in the endothelium may determine the regulation of vascular permeability by S1P. PMID: 17431187
38. antagonism of the S1P2R may be a novel therapeutic approach for the prevention and/or treatment of pathologic ocular neovascularization PMID: 17710232
39. These results suggest that S1P(2) receptors/G(12/13)-proteins/Rho signaling pathways mediate S1P-induced inhibition of glioma cell migration. PMID: 18088600
40. Results suggest that S1PR2 is involved in COX2 dependent effects of high-density lipoprotein on vascular smooth muscle. PMID: 18612546
41. Plays essential roles in the pathogenesis of rheumatoid arthritis by modulating fibroblast-like synoviocytes migration, cytokine/chemokine production, and cell survival. PMID: 18658144
42. impairment of function in senescent ECs in culture is mediated by an increase in S1P signaling through S1P(2)-mediated activation of the lipid phosphatase PTEN PMID: 18765664
43. These data suggest that CTGF protein induced by S1P2 might act as a growth inhibitor in Wilms' tumor. PMID: 18922980
44. the S1P(2) receptor is involved in S1P-induced platelet aggregation and Rho kinase activation. PMID: 19139947
45. S1P(2) signaling may play a critical role in suppressing diffuse large B-cell lymphoma PMID: 19903857
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相關疾病：

Deafness, autosomal recessive, 68 (DFNB68)
亞細胞定位：

Cell membrane; Multi-pass membrane protein.
蛋白家族：

G-protein coupled receptor 1 family
數據庫鏈接：

HGNC: 3169

OMIM: 605111

KEGG: hsa:9294

STRING: 9606.ENSP00000466933

UniGene: Hs.655405