Recombinant Human Ephrin-B2 (EFNB2)

in vitro E.coli expression system

中文名稱：

人EFNB2重組蛋白
貨號：

CSB-CF007466HU
說明書：

MSDS Datasheet
規格：
來源：

in vitro E.coli expression system
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

基因名：

EFNB2
Uniprot No.：

P52799
別名：

EFNB2; EPLG5; HTKL; LERK5; Ephrin-B2; EPH-related receptor tyrosine kinase ligand 5; LERK-5; HTK ligand; HTK-L
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length of Mature Protein
表達區域：

28-333
氨基酸序列

IVLEPIYWNSSNSKFLPGQGLVLYPQIGDKLDIICPKVDSKTVGQYEYYKVYMVDKDQADRCTIKKENTPLLNCAKPDQDIKFTIKFQEFSPNLWGLEFQKNKDYYIISTSNGSLEGLDNQEGGVCQTRAMKILMKVGQDASSAGSTRNKDPTRRPELEAGTNGRSSTTSPFVKPNPGSSTDGNSAGHSGNNILGSEVALFAGIASGCIIFIVIIITLVVLLLKYRRRHRKHSPQHTTTLSLSTLATPKRSGNNNGSEPSDIIIPLRTADSVFCPHYEKVSGDYGHPVYIVQEMPPQSPANIYYKV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.; (Microbial infection) Acts as a receptor for Hendra virus and Nipah virus.
基因功能參考文獻：
1. This is the novel report describing to demonstrate that the Runx2 expression of MSC is synergistically influenced by the hydrogels elasticity and their manner of ephrinB2 immobilized. PMID: 28300720
2. the binding of the viral attachment protein G to its host receptor ephrinB2 was studied and showed monomeric and dimeric receptors activate distinct conformational changes in G. PMID: 28974687
3. results suggest that ephrin-B2 overexpression and activation of the ephrin-B2 reverse signaling pathway in tumor microenvironment in OSCC facilitates progression and lymph node metastasis via enhancement of malignant potential and interaction with surrounding cells PMID: 29190834
4. Proteomic analysis of their secretome reveals a signature, including ephrin-B2, that predicts decreased survival of glioma patients. We find that ephrin-B2 is an important pro-angiogenic tenascin-C effector. PMID: 27926865
5. In human glioblastoma stem-like cells-derived orthotopic xenografts, EFNB2 knock-down blocked tumour initiation and treatment of established tumours with ephrin-B2-blocking antibodies suppressed progression. PMID: 27350048
6. roles in stabilizing vascular-like structures generated by stem cells from apical papilla (SCAPs) and umbilical vein endothelial cells (HUVECs) PMID: 27451120
7. High EFNB2 expression is associated with preeclampsia. PMID: 27069008
8. Inhibition of EphB4 forward signaling using soluble EphB4 protein fused with murine serum albumin failed to affect eRMS model tumor progression, but did moderately slow progression in murine aRMS PMID: 28817624
9. Study shows that EFNB2 is overexpressed in head and neck squamous cell carcinoma, pancreatic adenocarcinoma, and urothelial bladder carcinoma, and its overexpression correlates with poor survival of the cancer patients. PMID: 27649287
10. Five SNPs in the 3' region of the EFNB2 were in linkage disequilibrium and significantly associated with hypertension for male but not female subjects. PMID: 27530629
11. These results indicate a novel mechanism of ephrin-Eph signaling independent of direct cell contact and proteolytic cleavage and suggest the participation of EphB2(+)extracellular vesicles in neural development and synapse physiology. PMID: 27354374
12. Association of EFNB2 rs9520087 with symptoms of schizophrenia in Chinese Zhuang and Han populations. PMID: 27028544
13. miR-137 directly down-regulates the expression of EFNB2, and a genetic variant in the RNA binding site in EFNB2 gene affects the expression regulation. MiR-137, as a risk factor for schizophrenia PMID: 27650867
14. 5-fluorouracil-induced ephrin-B2 reverse signaling promotes tumorigenesis through the Src-ERK pathway, and drives EMT via the Src-FAK pathway. PMID: 26494468
15. Together, these findings suggest that CCL2, RARRES2 and EFNB2 are host cell factors involved in respiratory syncytial virus replication. PMID: 26277777
16. These genes may be potential biomarkers for identification of subclinical lymph node involvement in papillary thyroid carcinoma (PTC) PMID: 24634162
17. increased notably in preeclampsia endothelial progenitor cells and placenta PMID: 26212082
18. Results suggest a potential signalling hierarchy between Delta-like 1 and ephrin-B2 ligands, as neural stem cells adopt the Delta-like 1 phenotype of stem cell maintenance on simultaneous presentation of both signals. PMID: 26754526
19. role in maternal spiral artery remodeling in first trimester PMID: 25748802
20. EfnB2 is an essential regulator of endothelial cell death and vessel pruning. This regulation depends upon phosphotyrosine-EfnB2 signaling repressing JNK3 activity via STAT1. PMID: 25807892
21. Results suggest that ephrinB2 may function as a colorectal cancer cells growth stimulator. PMID: 25434750
22. Molecular recognition of human ephrinB2 cell surface receptor by an emergent African henipavirus. PMID: 25825759
23. Data suggest that fusion of Nipah viruses with host cells is facilitated by two of viral membrane proteins, the G protein and the F protein; G head domain binds to human ephrins B2 and B3 altering conformational density of entire G head domain. PMID: 24615845
24. Human ephrin-B2 is poorly cleaved by KLK4 while the homologous mouse is not. PMID: 25724897
25. Downregulation of the endothelial genes Notch1 and ephrinB2 in patients with nodular regenerative hyperplasia. PMID: 23870033
26. EphB4 and ephrin B2 play important roles in neovascularization and arteriovenous differentiation/plasticity. PMID: 24517927
27. Despite the negative results of our mutation screening, we still consider EFNB2 an excellent candidate gene for contributing to the development of Anorectal malformations in humans. PMID: 24038947
28. EphB2 and Ephrin-B2 interactions mediate human mesenchymal stem cell suppression of activated T-cells. PMID: 23711177
29. NNMT expression regulates neurone morphology in vitro via the sequential activation of the EFNB2 and Akt cellular signalling pathways. PMID: 23764850
30. High levels of ephrinB2 over-expression increases the osteogenic differentiation of human mesenchymal stem cells PMID: 23201622
31. EphrinB2 is an important indicator of poorly differentiated hepatocarcinoma. PMID: 22024229
32. Specific Ab-based ephrinB2 targeting may represent an effective therapeutic strategy to be used as an alternative or in combination with existing antiangiogenic drugs for treating patients with cancer and other angiogenesis-related diseases. PMID: 22446484
33. Monoclonal antibody developed in this study may be used as a reagent to probe ephrin-B2 distribution in normal as well as in pathological conditions and to antagonize ephrin-B2 interaction with EphB4 for basic science and therapeutic applications. PMID: 22292016
34. The expressions of ephrinB2 and EphB4 were significantly higher in NSCLC tissue than in control tissues, and were positively correlated with lymphatic metastasis. PMID: 21575518
35. deregulated ephrin-B2 expression interferes with the regulation of the stem cell niche and leads to a shift of the differentiation pathway and may thereby contribute to the acquisition of the metastatic phenotype PMID: 22020958
36. ephrin-B2-EphB4 signaling between PDLF and osteoblasts of the alveolar bone might contribute to osteogenesis at tension sites during orthodontic tooth movement PMID: 21880727
37. Results indicated that the G-H loop of ephrin-B2 was indeed critical for the interaction between ephrin-B2 and Nipah virus-G. PMID: 21632558
38. EphB4/ephrinB2 interactions between tumor cells and endothelial cells identify a mechanism for site-specific metastatic dissemination of tumor cells. PMID: 21047731
39. High ephrin-B2 is associated with malignant urogenital tissue. PMID: 19272799
40. The find of this study indicated that EFNB2 gene may be a candidate susceptibility gene for schizophrenia in the Han Chinese population. PMID: 20483485
41. The data indicate that MSCs expressing Ephrin-B2 represent a novel proangiogenic cell source to promote neovascularization in ischemic tissues. PMID: 20491587
42. Ephrin-B2 is a potent regulator of endothelial cell behavior, and indicate that the control of cell migration and angiogenesis by ephrins might involve both receptor-dependent and receptor-independent activities. PMID: 20233847
43. The crystal structure of the EphA4 ligand-binding domain in complex with ephrin-B2, is reported. PMID: 19875447
44. ephrin-B2 plays a critical role in glioma invasion PMID: 19728339
45. expressed differentially in colon carcinoma and normal mucosa specimens and may play a role in progression of colon carcinoma PMID: 11920461
46. Endogenous ephrin B2 from human umbilical artery endothelial cells activates a kinase that phosphorylates murine GST-ephrin-B1 cytoplasmic domain fusion proteins. PMID: 11983165
47. role of ephrin-B2 in erythropoiesis PMID: 12051776
48. The entire cytoplasmic domain of ephrin-B2 and its N-terminal fragment, residues 253-300, lack the ability to fold into a well-defined three dimensional structure and are particularly prone to aggregation. PMID: 12206665
49. These data identify distinct propulsive and repulsive effector functions of endothelial ephrinB2 and EphB4 that mediate spatial positional signals during angiogenesis and vessel assembly. PMID: 12734395
50. ephrin B2 is present in human retinal endothelial cells and vascular growth may be modulated in the retina through activation of the PI3K pathway. PMID: 14499344
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亞細胞定位：

Cell membrane; Single-pass type I membrane protein. Cell junction, adherens junction.
蛋白家族：

Ephrin family
組織特異性：

Lung and kidney.
數據庫鏈接：

HGNC: 3227

OMIM: 600527

KEGG: hsa:1948

STRING: 9606.ENSP00000245323

UniGene: Hs.149239