Recombinant Human Chemerin-like receptor 1 (CMKLR1)

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中文名稱：

重組人化學(xué)素樣受體1（CMKLR1）
品名簡稱：

Recombinant Human CMKLR1 protein
貨號(hào)：

CSB-CF005614HU
說明書：

MSDS Datasheet
規(guī)格：

￥9720
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

CMKLR1
Uniprot No.：

Q99788
別名：

CMKLR1; CHEMR23; DEZ; Chemerin-like receptor 1; Chemokine-like receptor 1; G-protein coupled receptor ChemR23; G-protein coupled receptor DEZ
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

in vitro E.coli expression system
分子量：

45.1 kDa
表達(dá)區(qū)域：

1-373aa
氨基酸序列

MRMEDEDYNTSISYGDEYPDYLDSIVVLEDLSPLEARVTRIFLVVVYSIVCFLGILGNGLVIIIATFKMKKTVNMVWFLNLAVADFLFNVFLPIHITYAAMDYHWVFGTAMCKISNFLLIHNMFTSVFLLTIISSDRCISVLLPVWSQNHRSVRLAYMACMVIWVLAFFLSSPSLVFRDTANLHGKISCFNNFSLSTPGSSSWPTHSQMDPVGYSRHMVVTVTRFLCGFLVPVLIITACYLTIVCKLQRNRLAKTKKPFKIIVTIIITFFLCWCPYHTLNLLELHHTAMPGSVFSLGLPLATALAIANSCMNPILYVFMGQDFKKFKVALFSRLVNALSEDTGHSSYPSHRSFTKMSSMNERTSMNERETGML
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 10xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產(chǎn)品描述：

Chemerin-like receptor 1（CMKLR1）也稱為ChemR23，是與趨化作用、炎癥和免疫應(yīng)答調(diào)節(jié)相關(guān)的G蛋白偶聯(lián)受體，在分子生物學(xué)和免疫學(xué)領(lǐng)域備受關(guān)注。CMKLR1在調(diào)節(jié)免疫細(xì)胞，尤其是樹突狀細(xì)胞和巨噬細(xì)胞向炎癥部位趨化方面發(fā)揮著關(guān)鍵作用。它與Chemerin的相互作用已知能夠調(diào)控免疫細(xì)胞的遷移和激活。該受體涉及到各種生理過程，包括脂肪生成、血管生成和代謝調(diào)控。
華美生物的重組人CMKLR1蛋白是無細(xì)胞表達(dá)系統(tǒng)表達(dá)全長蛋白，表達(dá)區(qū)域?yàn)?-82aa，該產(chǎn)品在體外實(shí)驗(yàn)、結(jié)合研究和功能實(shí)驗(yàn)中具有廣泛的應(yīng)用。為探究免疫應(yīng)答、炎癥和代謝調(diào)控等方面的分子機(jī)制提供了一個(gè)工具，可用于研究Chemerin-趨化作用相互作用、信號(hào)轉(zhuǎn)導(dǎo)途徑以及靶向CMKLR1在各種疾病中的潛在治療應(yīng)用研究等。
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Receptor for the chemoattractant adipokine chemerin/RARRES2 and for the omega-3 fatty acid derived molecule resolvin E1. Interaction with RARRES2 initiates activation of G proteins G(i)/G(o) and beta-arrestin pathways inducing cellular responses via second messenger pathways such as intracellular calcium mobilization, phosphorylation of MAP kinases MAPK1/MAPK3 (ERK1/2), TYRO3, MAPK14/P38MAPK and PI3K leading to multifunctional effects, like, reduction of immune responses, enhancing of adipogenesis and angionesis PubMed:27716822. Resolvin E1 down-regulates cytokine production in macrophages by reducing the activation of MAPK1/3 (ERK1/2) and NF-kappa-B. Positively regulates adipogenesis and adipocyte metabolism.; (Microbial infection) Acts as a coreceptor for several SIV strains (SIVMAC316, SIVMAC239, SIVMACL7E-FR and SIVSM62A), as well as a primary HIV-1 strain (92UG024-2).
基因功能參考文獻(xiàn)：
1. levels in cord blood, peripheral blood, adipose tissue and placenta tissue significantly higher in gestational diabetes PMID: 29430984
2. CMKLR1 and GPR1 were widely expressed in vascular smooth muscle. PMID: 27742615
3. Both ALX and ChemR23 were present in human synovium and medial tibial plateau bone obtained following total knee replacement surgery for osteoarthritis. PMID: 27860453
4. Results show that ChemR23 is highly expressed in squamous oesophageal cancer tumors and cell lines. PMID: 27092781
5. CMKLR1 exacerbated the proliferation and migration of VSMCs by activating ERK1/2. PMID: 27792688
6. We demonstrated that chemerin is linked to metabolic syndrome components. Moreover, serum chemerin levels were associated significantly with obesity, especially visceral adipose tissue, in subjects with T2DM [type 2 diabetes mellitus]. PMID: 28120562
7. Study shows that hepatic CMKLR1 mRNA is weakly associated with features of NASH in male patients only. PMID: 27548138
8. Our results show that higher levels of circulating chemerin, CRP, fibrinogen, and ESR are associated with an increased risk of developing colorectal cancer PMID: 26628300
9. Data show inverse Relationship of the CMKLR1 Relative Expression and Chemerin Serum Levels in Obesity with Dysmetabolic Phenotype and Insulin Resistance PMID: 27239101
10. Results indicate that Abeta42 activates CMKLR1, leading to glia cell migration and clearance of Abeta42; and is involved in Abeta processing and clearance PMID: 25079809
11. Gestational obesity and gestational diabetes mellitus may contribute to elevated serum chemerin. Serum chemerin in pregnancy was associated with insulin resistance and triglycerides. Chemerin gene may play a role both in obese and gestational diabetes mellitus patients PMID: 25627894
12. Increased chemerin expression in dermal blood vessels may be associated with the development of digital ulcers in systemic sclerosis. PMID: 25539827
13. The study for the first time confirmed a marked expression of chemerin and CMKLR1 in the liver of chronic hepatitis patients. PMID: 25121101
14. ChemR23, the receptor for chemerin and resolvin E1, is expressed and functional on M1 but not on M2 macrophages. PMID: 25637017
15. The effects of diclofenac on the incidence of pancreatitis following endoscopic retrograde cholangiopancreatography via lipoxin A4 and resolvin D1 and E1 levels is reported. PMID: 25030943
16. Results lend some support to a presumable role of locally produced chemerin in the progression of atherosclerotic lesions, possibly acting through its CMKLR1 receptor. PMID: 24779513
17. This study identified the up-regulation of ChemR23 in post-traumatic injury of calcaneal articular fracture PMID: 24689495
18. ChemR23 is expressed in neutrophil granules and rapidly upregulated upon neutrophil activation. PMID: 23999103
19. Development of a membrane-anchored chemerin receptor agonist as a novel modulator of allergic airway inflammation and neuropathic pain. PMID: 24659779
20. Chemerin receptor showed increased expression in the lymph nodes and the spleen. PMID: 23904282
21. Prochemerin processing protease converts prochemerin into active chemerinF; the activating truncation by the protease may trigger a structural C-terminal rearrangement leading to increased affinity of chemerin to chemokine-like receptor (CMKLR)1. PMID: 23495698
22. review of current research on biological roles of chemerin and chemokine-like receptor 1; highlight roles in mediating obesity and development of type 2 diabetes [REVIEW] PMID: 22610747
23. The interaction of chemerin and ChemR23 may play an important role in the pathogenesis of rheumatoid arthritis through the resultant activation of fibroblast-like synoviocytes. PMID: 21959042
24. These results rule out the direct anti-inflammatory effect of chemerin on macrophages ex vivo, described previously in the literature, despite the expression of a functional ChemR23 receptor in these cells. PMID: 22768214
25. Chemerin, a ligand for the G-protein coupled receptor chemokine-like receptor 1, requires carboxy-terminal proteolytic processing to unleash its chemoattractant activity PMID: 21715684
26. The ChemR23/Chemerin axis may have a role in the recruitment of dendritic cells within the kidney in patients affected by lupus nephritis. PMID: 21346723
27. A genetic variation in the chemokine-like receptor 1 (CMKLR1) gene was statistically significantly associated with decreased overall survival in the three individual populations as well as in pooled analyses. PMID: 21483023
28. These results demonstrate that human chondrocytes express both the receptor ChemR23 and the ligand chemerin...which may play pivotal roles in joint inflammation. PMID: 21192818
29. the presence of ChemR23 in human endothelial cells, its significant up-regulation by pro-inflammatory cytokines, and its role in angiogenesis were demonstrated. PMID: 20044979
30. RvE1 initiates direct activation of ChemR23 and signals receptor-dependent phosphorylation PMID: 19906641
31. ChemR23 mediates the Resolvin E1 signal to attenuate nuclear factor-kappaB PMID: 15753205
32. The results strongly support that the chemerin receptor, in the presence of CD4, functions as a "minor co-receptor" promoting infection by HIV-1, HIV-2 and SIV. PMID: 16904155
33. CMKLR1 is expressed by circulating pDC in normal individuals and patients suffering from skin diseases, such as psoriasis and atopic dermatitis. PMID: 19168032
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亞細(xì)胞定位：

Cell membrane; Multi-pass membrane protein.
蛋白家族：

G-protein coupled receptor 1 family
組織特異性：

Prominently expressed in developing osseous and cartilaginous tissue. Also found in adult parathyroid glands. Expressed in cardiovascular system, brain, kidney, gastrointestinal tissues and myeloid tissues. Expressed in a broad array of tissues associated
數(shù)據(jù)庫鏈接：

HGNC: 2121

OMIM: 602351

KEGG: hsa:1240

STRING: 9606.ENSP00000311733

UniGene: Hs.197143