Recombinant Human Calcium uniporter protein, mitochondrial (MCU)

in vitro E.coli expression system

中文名稱：

人MCU重組蛋白
貨號：

CSB-CF847694HU
說明書：

MSDS Datasheet
規格：
來源：

in vitro E.coli expression system
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

基因名：

MCU
Uniprot No.：

Q8NE86
別名：

MCU; C10orf42; CCDC109A; Calcium uniporter protein, mitochondrial; HsMCU; Coiled-coil domain-containing protein 109A
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length of Mature Protein
表達區域：

51-351
氨基酸序列

VHQRIASWQNLGAVYCSTVVPSDDVTVVYQNGLPVISVRLPSRRERCQFTLKPISDSVGVFLRQLQEEDRGIDRVAIYSPDGVRVAASTGIDLLLLDDFKLVINDLTYHVRPPKRDLLSHENAATLNDVKTLVQQLYTTLCIEQHQLNKERELIERLEDLKEQLAPLEKVRIEISRKAEKRTTLVLWGGLAYMATQFGILARLTWWEYSWDIMEPVTYFITYGSAMAMYAYFVMTRQEYVYPEARDRQYLLFFHKGAKKSRFDLEKYNQLKDAIAQAEMDLKRLRDPLQVHLPLRQIGEKD
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria. Constitutes the pore-forming and calcium-conducting subunit of the uniporter complex (uniplex). Activity is regulated by MICU1 and MICU2. At low Ca(2+) levels MCU activity is down-regulated by MICU1 and MICU2; at higher Ca(2+) levels MICU1 increases MCU activity. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways. Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes. While dispensable for baseline homeostatic cardiac function, acts as a key regulator of short-term mitochondrial calcium loading underlying a 'fight-or-flight' response during acute stress: acts by mediating a rapid increase of mitochondrial calcium in pacemaker cells. participates in mitochondrial permeability transition during ischemia-reperfusion injury. Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake. Mitochondrial calcium uptake in skeletal muscle cells is involved in muscle size in adults. Regulates synaptic vesicle endocytosis kinetics in central nerve terminal. Involved in antigen processing and presentation.
基因功能參考文獻：
1. MICU1 imparts the mitochondrial uniporter with the ability to discriminate between Ca(2+) and Mn(2+). PMID: 30082385
2. Mitochondrial Ca(2+) uptake is controlled by protein arginine methyl transferase 1 that asymmetrically methylates MICU1, resulting in decreased Ca(2+) sensitivity. UCP2/3 normalize Ca(2+) sensitivity of methylated MICU1 and, thus, re-establish mitochondrial Ca(2+) uptake activity. PMID: 27642082
3. VDAC1 allows Ca(2+) access to the MCU, facilitating transport of Ca(2+) to the matrix, and also from the IMS to the cytosol. Intra-mitochondrial Ca(2+) controls energy production and metabolism by modulating critical enzymes in the tricarboxylic acid (TCA) cycle and fatty acid oxidation. PMID: 29594867
4. MICU2 restricts spatial crosstalk between InsP3R and MCU channels by regulating threshold and gain of MICU1-mediated inhibition and activation of MCU. PMID: 29241542
5. The results highlight the dynamic nature of uniporter subunit assembly, which must be tightly regulated to ensure proper mitochondrial responses to intracellular Ca(2+) signals. PMID: 28396416
6. MCU expression returned to physiological levels in visceral adipose tissue of patients after weight loss by bariatric surgery. Altered mitochondrial calcium flux in fat cells may play a role in obesity and diabetes and may be associated with the differential metabolic profiles of visceral and subcutaneous adipose tissue. PMID: 28790027
7. Mitochondrial calcium uniporter plays an important role in hyperglycaemia-induced endothelial cell dysfunction. PMID: 28777009
8. The studies findings in aging human skeletal muscle confirm the data obtained in mice and propose mitochondrial calcium uniporter and mitochondria-related proteins as potential pharmacological targets to counteract age-related muscle loss. PMID: 28039397
9. decreased MCU expression in hypertensive with mutation cells contributed to dysregulated Ca(2+) uptake into the mitochondria PMID: 27471128
10. The molecular structure and regulation of the MCU complex in addition to its pathophysiological role are discussed with particular attention to striated muscle tissues. Review. PMID: 26968367
11. High MCU expression is associated with metastasis in hepatocellular carcinoma. PMID: 28650465
12. Here, the authors determine the transmembrane orientation of EMRE, and show that its known MCU-activating function is mediated by the interaction of transmembrane helices from both proteins. PMID: 27099988
13. Regulation of mitochondrial Ca(2+) suggests that MCU may play a pivotal role in the development of fibrosis and could potentially be a therapeutic target for pulmonary fibrosis. PMID: 28351840
14. MCU downregulation hampered cell motility and invasiveness and reduced tumor growth, lymph node infiltration, and lung metastasis in triple-negative breast cancer xenografts. In MCU-silenced cells, production of mitochondrial reactive oxygen species (mROS) is blunted and expression of the hypoxia-inducible factor-1alpha (HIF-1alpha) is reduced, suggesting a signaling role for mROS and HIF-1alpha, downstream of mitochon... PMID: 27138568
15. Reveal a distinct functional role for Cys-97 in mitochondrial reactive oxygen species sensing and regulation of MCU activity. PMID: 28262504
16. Study indicates that inhibition of mitochondrial calcium uniporter can inhibit excessive mitophagy and protect the neurocytes from ischemia/reperfusion injury. PMID: 27288019
17. Our experiments provide novel details about how MCU/EMRE is regulated by MICU1 and an original approach to investigate MCU/EMRE activation in intact cells. PMID: 26489515
18. results suggest that N terminal domain of MCU is essential for the modulation of MCU function, although it does not affect the uniplex formation PMID: 26341627
19. MCU-VDAC1 complex regulates mitochondrial Ca(2+) uptake and oxidative stress-induced apoptosis PMID: 25753332
20. Loss of heterozygosity of MCU gene on chromosome 10q is associated with pancreatic cancer. PMID: 25824785
21. Studies indicate the existence of an inner mitochondrial protein termed the mitochondrial calcium uniporter for calcium transport. PMID: 25999421
22. analyses establish that MCU encodes the pore-forming subunit of the uniporter channel PMID: 23755363
23. MCU plays a critical role in breast cancer cell migration by regulating store-operated Ca(2) entry. PMID: 25640838
24. We review not only the biochemical identities and structures of the proteins required for mitochondrial Ca2+ uptake but also their implications in different physiopathological contexts. [review] PMID: 24366263
25. ERp57 can regulate the expression of the mitochondrial calcium uniporter (MCU) and modulate mitochondrial calcium uptake PMID: 24815697
26. study demonstrates that MCU overexpression is a feature of some breast cancers and that MCU overexpression may offer a survival advantage against some cell death pathways PMID: 23602897
27. The mitochondrial calcium uniporter (MCU): molecular identity and physiological roles. PMID: 23400777
28. the crucial role of MICU1 and MCU in mitochondrial Ca(2+) uptake in pancreatic beta-cells and their involvement in the positive feedback required for sustained insulin secretion. PMID: 22904319
29. Functional characterization of the MCU protein; shows that it is necessary for mitochondrial calcium uptake. PMID: 21685888
30. MCU is an oligomeric protein residing in the mitochondrial inner membrane that interacts with MICU1 and is necessary for mitochondrial calcium uniport. PMID: 21685886
31. CCDC109A was identified in this large-scale proteomics analysis as a mitochondrial protein broadly expressed in many mouse tissues. The human protein was also confirmed to localize to mitochondria. PMID: 18614015
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亞細胞定位：

Mitochondrion inner membrane; Multi-pass membrane protein.
蛋白家族：

MCU (TC 1.A.77) family
數據庫鏈接：

HGNC: 23526

OMIM: 614197

KEGG: hsa:90550

STRING: 9606.ENSP00000362144

UniGene: Hs.591366