Recombinant Human Beta-1,4-galactosyltransferase 1 (B4GALT1)

in vitro E.coli expression system

中文名稱：

Recombinant Human Beta-1,4-galactosyltransferase 1(B4GALT1)
貨號：

CSB-CF002513HU
說明書：

MSDS Datasheet
規格：
來源：

in vitro E.coli expression system
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

基因名：

B4GALT1
Uniprot No.：

P15291
別名：

B4GALT1; GGTB2; Beta-1,4-galactosyltransferase 1; Beta-1,4-GalTase 1; Beta4Gal-T1; b4Gal-T1; Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase; Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase; Lactose synthase A protein; N-acetyllactosamine synthase; Nal synthase; Neolactotriaosylceramide beta-1,4-galactosyltransferase; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1
種屬：

Homo sapiens (Human)
蛋白長度：

full length protein
表達區域：

1-398
氨基酸序列

MRLREPLLSGSAAMPGASLQRACRLLVAVCALHLGVTLVYYLAGRDLSRLPQLVGVSTPLQGGSNSAAAIGQSSGELRTGGARPPPPLGASSQPRPGGDSSPVVDSGPGPASNLTSVPVPHTTALSLPACPEESPLLVGPMLIEFNMPVDLELVAKQNPNVKMGGRYAPRDCVSPHKVAIIIPFRNRQEHLKYWLYYLHPVLQRQQLDYGIYVINQAGDTIFNRAKLLNVGFQEALKDYDYTCFVFSDVDLIPMNDHNAYRCFSQPRHISVAMDKFGFSLPYVQYFGGVSALSKQQFLTINGFPNNYWGWGGEDDDIFNRLVFRGMSISRPNAVVGRCRMIRHSRDKKNEPNPQRFDRIAHTKETMLSDGLNSLTYQVLDVQRYPLYTQITVDIGTPS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.; The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix.
基因功能參考文獻：
1. Human beta-1,4-GalT1 is a constitutively expressed mesangial cell IgA receptor with an important role in both mesangial IgA clearance and the initial response to IgA deposition. PMID: 28750925
2. Increased B4GALT1 expression is a potential independent adverse prognostic factor for overall survival in patients with non-metastatic clear cell renal cell carcinoma. PMID: 27092876
3. demonstrated that ZFX is aberrantly expressed in multiple human leukemic cells and it modulates the growth and drug response of leukemic cells partially via B4GALT1, which suggests that ZFX is a new regulator of leukemic cells and warrants intensive investigations on this 'stemness' regulator in these deadly diseases PMID: 27797721
4. High B4GALT1 expression is associated with aging. PMID: 26840264
5. We suggest that the unique expression patterns for the B4GALT1 in normal and malignant tissues are controlled by a differential usage of 5'-B4GALT1 regulatory units along with a post-transcriptional regulation by the antisense RNA PMID: 26315939
6. Human chorionic gonadotropin provides a mechanism to bridge embryo to endometrium through beta1,4-GalT. PMID: 26191157
7. HS5 cells had significantly enhanced levels of bisecting N-glycans (catalyzed by MGAT3 whereas HS27a cells had enhanced levels of Galbeta1,4GlcNAc. PMID: 25936519
8. plays a role in activation of T-lymphocytes and participates in intercellular contact formation PMID: 25223470
9. B4GALT1 and B4GALT5, two members of B4GALT gene family, are involved in the development of multidrug resistance of human leukemia cells. PMID: 23744354
10. the glycogene B4GALT1 represent a valuable candidate biomarker of invasive phenotype of colorectal cancer. PMID: 22927297
11. RNAi-mediated knockdown of beta1,4GT1 increased the levels of EGFR dimerization and phosphorylation. These data suggest that cell surface beta1,4GT1 interacts with EGFR and inhibits EGFR activatio PMID: 23583406
12. estrogen regulates the expression of B4GALT1 through the direct binding of ER-alpha to ERE; the expressed B4GALT1 plays a crucial role in the proliferation of MCF-7 cells through its activity as a membrane receptor. PMID: 22982306
13. Osteopontin increases the expression of beta1, 4-Galactosyltransferase-I and promotes adhesion in human RL95-2 cells, the activity critical for embryo implantation. PMID: 22847114
14. expression of beta1,4-GalT-I increased in the cartilage and synovial tissue of osteoarthritis (OA) patients compared with healthy controls; data suggest that beta1,4-GalT-I may play an important role in the inflammatory processes in cartilage and synovial tissue of patients with OA PMID: 21750942
15. beta4Gal-T1 molecule has two different oligosaccharide binding regions for the binding of the extended oligosaccharide moiety of the acceptor substrate. PMID: 22740701
16. TNF-alpha contributes to the up-regulation of beta1,4-GalT-I mRNA in human fibroblast-like synoviocytes. PMID: 20886274
17. These results suggested that beta1,4-Galactosyltransferase-I may play an important role in the inflammatory processes in synovial tissue of patients with rheumatoid arthritis. PMID: 20490888
18. B4GalTI might act as a key adhesion molecule participating in T cell-dendritic cell contacts. PMID: 20851383
19. PKC signal transduction pathway participates in regulating beta-1, 4-GalT-I expression in endothelial cells stimulated by TNF-alpha. PMID: 20619088
20. The mutation of C80S introduces a fully occupied UDP binding site at the enzyme dimer interface that is observed to be dependent on the binding of H antigen acceptor analog. PMID: 20655926
21. Golgi N-glycosyltransferases beta-1,2-N-acetylglucosaminyltransferase I, beta-1,2-N-acetylglucosaminyltransferase II, 1,4-galactosyltransferase I, and alpha-2,6-sialyltransferase I form both homo- and heterodimeric enzyme complexes in live cells PMID: 20378551
22. found that both the long and short isoforms of beta-1,4-GalT-I were expressed in human CD4(+) T lymphocytes, and localized in the cytoplasm and on the plasma membrane. PMID: 20202494
23. deficiency causes a new congenital disorder of glycosylation (CDG), designated type IId (CDG-IId), a severe neurologic disease characterized by hydrocephalus, myopathy, and blood-clotting defects PMID: 11901181
24. TNFalpha modulates the glycosylation of endothelial cells by a mechanism that directly enhances the stability of beta4GalT-1 mRNA transcripts PMID: 15668241
25. beta4Gal-T1 interacts preferentially with the 1,2-1,6-arm trisaccharide rather than with the 1,2-1,3-arm or 1,4-1,3-arm of a bi- or tri-antennary oligosaccharide chain of N-glycan PMID: 16157350
26. Data show that expression of a hybrid enzyme of Arabidopsis thaliana xylosyltransferase and human beta-1,4-galactosyltransferase I in tobacco causes a reduction of N-glycans with potentially immunogenic core-bound xylose and fucose residues. PMID: 16675551
27. These results demonstrated that cell surface beta1,4GT1 may negatively regulate cell survival possibly through inhibiting and modulating EGFR signaling pathway. PMID: 16786197
28. Cycling between the trans-Golgi cisterna and the trans-Golgi network of galT is signal mediated. PMID: 17021253
29. hepatitis B-induced GalT I expression might contribute to hepatitis B-mediated hepatocellular carcinoma development and progression PMID: 18929424
30. Deoxygenated disaccharide analogs as specific inhibitors of beta1-4-galactosyltransferase 1 and selectin-mediated tumor metastasis. PMID: 19106107
31. These results demonstrate that B3GNT1 and B4GALT1 physically associate in vitro and in cultured cells, providing insight into possible mechanisms for regulation of polyLacNAc production. PMID: 19261593
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相關疾病：

Congenital disorder of glycosylation 2D (CDG2D)
亞細胞定位：

[Isoform Long]: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein. Cell surface. Cell projection, filopodium.; [Isoform Short]: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein.; [Processed beta-1,4-galactosyltransferase 1]: Secreted.
蛋白家族：

Glycosyltransferase 7 family
組織特異性：

Ubiquitously expressed, but at very low levels in fetal and adult brain.
數據庫鏈接：

HGNC: 924

OMIM: 137060

KEGG: hsa:2683

STRING: 9606.ENSP00000369055

UniGene: Hs.272011