Recombinant Human 5-hydroxytryptamine receptor 4 (HTR4)

中文名稱：

Recombinant Human 5-hydroxytryptamine receptor 4 (HTR4)
品名簡稱：

Recombinant Human HTR4 protein
貨號：

CSB-CF622677HU
說明書：

MSDS Datasheet
規格：
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

HTR4
Uniprot No.：

Q13639
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

in vitro E.coli expression system
分子量：

45.3 kDa
表達區域：

1-388aa
氨基酸序列

MDKLDANVSSEEGFGSVEKVVLLTFLSTVILMAILGNLLVMVAVCWDRQLRKIKTNYFIVSLAFADLLVSVLVMPFGAIELVQDIWIYGEVFCLVRTSLDVLLTTASIFHLCCISLDRYYAICCQPLVYRNKMTPLRIALMLGGCWVIPTFISFLPIMQGWNNIGIIDLIEKRKFNQNSNSTYCVFMVNKPYAITCSVVAFYIPFLLMVLAYYRIYVTAKEHAHQIQMLQRAGASSESRPQSADQHSTHRMRTETKAAKTLCIIMGCFCLCWAPFFVTNIVDPFIDYTVPGQVWTAFLWLGYINSGLNPFLYAFLNKSFRRAFLIILCCDDERYRRPSILGQTVPCSTTTINGSTHVLRDAVECGGQWESQCHPPATSPLVAAQPSDT
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase.
基因功能參考文獻：
1. found that testosterone, but not estradiol, correlated negatively with global 5-HT4R levels suggesting that men with high levels of testosterone have higher cerebral serotonergic tonus. PMID: 28426945
2. l-Lysine significantly reduced the surge of plasma aldosterone induced by metoclopramide indicating that l-Lysine is able to efficiently antagonize the adrenal 5-HT4 receptors in vivo. PMID: 28103616
3. Acute administration of 5-hydroxytryptamine4 (5-HT4) receptor agonist, mosapride or esophageal infusion of the transient receptor potential vanilloid receptor-1 (TRPV1) agonist capsaicin promotes secondary peristalsis.Esophageal infusion with capsaicin-containing red pepper sauce suspension does create greater mechanosensitivity as measured by secondary peristalsis than 5-HT4 receptor agonist mosapride PMID: 27438088
4. Findings replicate our previous observation of a negative association between 5-HT 4R binding and memory performance in an independent cohort and provide novel evidence linking 5-HT 4R binding, as a biomarker for synaptic 5-HT levels, to the mnestic processing of positive and neutral word stimuli in healthy humans PMID: 28413715
5. Results of this study indicated that HTR4 signaling upregulated ERbeta expression in hormone-naive prostate cancer and could impact on biological processes in hormone-naive prostate cancer. PMID: 28195088
6. Data suggest that the 5-HT4 receptor is involved in the neurobiological mechanism underlying familial risk for depression, and that lower striatal 5-HT4 receptor binding is associated with increased risk for developing major depression disorder PMID: 25522384
7. PDE3A1 and PDE4D3 are integrated into complexes that contain the 5-HT4(b) receptor and may thereby regulate 5-HT4(b) receptor-mediated signaling. PMID: 25101859
8. The findings of altered lung function and increased AHR in Htr4-null mice support a causal relationship between genetic variation in HTR4 and pulmonary function identified in human genome-wide association studies. PMID: 25342126
9. This study demonistrated that 5-hydroxytryptamine (serotonin) receptor 4 regulate memory. PMID: 25622143
10. ADAM19 rs1422795 and HTR4 rs11168048 are associated with pulmonary function. PMID: 24951661
11. This is the first transgenic model to study human 5-HTreceptor in the atrium ex vivo or in vivo. PMID: 23307014
12. Stimulation of central 5-HT4 receptors is potentially disease-modifying in a transgenic mouse model of Alzheimer's disease. PMID: 23474291
13. Taken together, these data suggest a role for HTR4 in lung development, which may at least in part explain the genetic association with lung function. PMID: 23890215
14. Our results suggest that HTR4 polymorphisms may not play a major role in the susceptibility for suicidal behavior in subjects with schizophrenia. PMID: 22842674
15. These results suggest a prominent role of 5-HT(4)R in promoting angiogenesis. PMID: 22903372
16. Results suggest an important role for the CHRNA5/3 region as a genetic risk factor for airflow obstruction independent of smoking and implicate the HTR4 gene in the etiology of airflow obstruction. PMID: 22837378
17. Our findings are consistent with a model wherein the 5-HTTLPR S allele is associated with relatively increased serotonin levels. PMID: 22584237
18. These findings suggest that the 5-HT(4)R is critically involved in reward circuits that regulate people's food intake PMID: 22709820
19. Mucosal 5-HT(4) receptor activation can mediate the prokinetic and antinociceptive actions of 5-HT(4)R agonists. PMID: 22226658
20. The occurrence of heterogeneous post-translational modifications (PTMs) on transgenic proteins, as well as the complications that non-native PTMs can cause, highlight the characterization of both endogenous and heterologous protein targets. PMID: 22145929
21. 5-HT4 receptor binding was a positively correlated to amyloid beta (A4) burden and negatively to MMSE score of the Alzheimer disease patients PMID: 21673407
22. HTR4 polymorphism associated with COPD risk and lung function decline PMID: 21965014
23. SNP and haplotypes of the HTR4 gene were associated with the asthma phenotype and genetic variation of HTR4 may affect susceptibility to the development of asthma. PMID: 21382128
24. The relatively stable HT4 5- receptor binding with aging contrasts others in subtypes of receptors, which generally decrease with aging. PMID: 21364600
25. This study demonistrated that 5-HT4 receptor is present in the human thalamus prenatally. PMID: 20538346
26. No association was found in the family sample betweewn HTR4 and schizophrenia. PMID: 19892407
27. Data show expression of TNS1, GSTCD, AGER, HTR4 and THSD4 in lung tissue and indicate potential targets for interventions to alleviate respiratory disease. PMID: 20010834
28. Computational model of the complex between GR113808 and the 5-HT4 receptor guided by site-directed mutagenesis and the crystal structure of rhodopsin. PMID: 11989623
29. The polymorphisms associated with mood disorder were located within the region that encodes the divergent C-terminal tails of the 5-HT(4) receptor. PMID: 12399948
30. report the activation of the extracellular signal-regulated kinases (ERKs) 1 and 2 (p44 and p42 MAP kinase) through the human serotonin receptors 5-HT(4(b)) and 5-HT(7(a)) PMID: 12446729
31. results show an overexpression of the 5-HT4 receptor in cisapride-responsive ACTH-independent bilateral macronodular adrenal hyperplasia PMID: 12519861
32. A highly significant association between schizophrenia and haplotype A-T (OR = 0.13 [0.03-0.58]) was detected. PMID: 12898568
33. Our results suggest a complex regulation of the h5-HT4 receptor gene expression involving distinct promoters and non-coding exons. PMID: 15575821
34. secretion of the non-amyloidogenic form of amyloid precursor protein, sAPPalpha induced by the 5-HT4(e) receptor isoform was not due to a general boost of the constitutive secretory pathway but rather to its specific effect on alpha-secretase activity PMID: 15710402
35. inhibition of Na+/H+ exchange activity by serotonin is mediated by 5-HT4 receptors in Caco-2 cells PMID: 15825078
36. coexpression of h5-HT4R and beta2-adrenergic receptor (beta2AR) led to their heterodimerization PMID: 15896782
37. the uncoupling and endocytosis of 5-HT4R require different GRK2 concentrations and involve distinct molecular events PMID: 15919661
38. development of an adenovirus expression system to examine the properties of two human 5-HT4 receptor splice variants, h5-HT4(b) and h5-HT4(d), expressed in adult cardiomyocytes devoid of native 5-HT4 receptors PMID: 15950987
39. These findings provide the first evidence of differential internalization between the two splice variants, 5-HT(4a) and 5-HT(4b) receptors. PMID: 16209130
40. We show overexpression & different splicing of 5-HT4 receptor in aldosterone-producing adenoma tissues in comparison with normal adrenocortical tissue.Isoforms (a) & (b) were not expressed in any APA but were present in majority of normal adrenal cortex. PMID: 16322401
41. These results suggest that the HTR4 gene may play a role in the genetic predisposition to ADHD. PMID: 16563621
42. Data show that 5-HT(4) receptor stimulation in primary neurons produced a potent but transient activation of the ERK pathway that is dependent on Src tyrosine kinase but totally independent of beta-arrestin. PMID: 17377064
43. We show that disulfide bridges between Cys112 and Cys145 located within transmembrane domain 3 and transmembrane domain 4, respectively, are of critical importance for 5-HT(4)R dimer formation. PMID: 17379184
44. Our results indicate that h5-HT(4(b)) is the dominant cardiac isoform of human 5-HT(4) receptors and its expression is increased in CAF. PMID: 17418812
45. Functional activiy of 5-HT4 receptors was studied in children with congenital heart disease. PMID: 17603679
46. In this review, the pathways regulating either beta- or gamma-secretase may be differentially controlled by 5-HT4 receptor isoforms. PMID: 18322379
47. In failing human ventricle, 5-HT(4) receptor-mediated positive inotropic response was regulated by PDEs in a manner similar to that in postinfarction rat hearts;5-HT, PDE3 and PDE4 may have pathophysiological functions in heart failure. PMID: 18846035
48. 5-HT4 expression is lower in heart ventricle vs. atrium. Isoform expression in atrium and ventricle is similar. There is a parallel increase of cAMP and in the phosphorylation state of regulatory proteins following stimulation with 5-HT in the atrium. PMID: 19002436
49. The human 5HT4 receptor, when placed under the influence of the mouse opsin promoter and an opsin rod outer segment (ROS) targeting sequence, localized to ROS of transgenic mouse retina. PMID: 19053287
50. This paper presents an analysis of the SH3TC2 promoter after identifying a read-through transcript of the SH3TC2 and HTR4 loci. Available data suggests HTR4 is a separate locus with its own promoter, and not the product of a bi-cistronic transcript. PMID: 11716477
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亞細胞定位：

Cell membrane; Multi-pass membrane protein. Endosome.
蛋白家族：

G-protein coupled receptor 1 family
組織特異性：

Isoform 5-HT4(A) is expressed in ileum, brain, and atrium, but not in the ventricle.
數據庫鏈接：

HGNC: 5299

OMIM: 602164

KEGG: hsa:3360

UniGene: Hs.483773