Recombinant Human 5,6-dihydroxyindole-2-carboxylic acid oxidase (TYRP1)

in vitro E.coli expression system

中文名稱：

人TYRP1重組蛋白
貨號：

CSB-CF025399HU
說明書：

MSDS Datasheet
規格：
來源：

in vitro E.coli expression system
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

基因名：

TYRP1
Uniprot No.：

P17643
別名：

TYRP1; CAS2; TYRP; TYRRP; 5,6-dihydroxyindole-2-carboxylic acid oxidase; DHICA oxidase; Catalase B; Glycoprotein 75; Melanoma antigen gp75; Tyrosinase-related protein 1; TRP; TRP-1; TRP1
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length of Mature Protein
表達區域：

25-537
氨基酸序列

QFPRQCATVEALRSGMCCPDLSPVSGPGTDRCGSSSGRGRCEAVTADSRPHSPQYPHDGRDDREVWPLRFFNRTCHCNGNFSGHNCGTCRPGWRGAACDQRVLIVRRNLLDLSKEEKNHFVRALDMAKRTTHPLFVIATRRSEEILGPDGNTPQFENISIYNYFVWTHYYSVKKTFLGVGQESFGEVDFSHEGPAFLTWHRYHLLRLEKDMQEMLQEPSFSLPYWNFATGKNVCDICTDDLMGSRSNFDSTLISPNSVFSQWRVVCDSLEDYDTLGTLCNSTEDGPIRRNPAGNVARPMVQRLPEPQDVAQCLEVGLFDTPPFYSNSTNSFRNTVEGYSDPTGKYDPAVRSLHNLAHLFLNGTGGQTHLSPNDPIFVLLHTFTDAVFDEWLRRYNADISTFPLENAPIGHNRQYNMVPFWPPVTNTEMFVTAPDNLGYTYEIQWPSREFSVPEIIAIAVVGALLLVALIFGTASYLIRARRSMDEANQPLLTDQYQCYAEEYEKLQNPNQSVV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Plays a role in melanin biosynthesis. Catalyzes the oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid in the presence of bound Cu(2+) ions, but not in the presence of Zn(2+). May regulate or influence the type of melanin synthesized. Also to a lower extent, capable of hydroxylating tyrosine and producing melanin.
基因功能參考文獻：
1. The rs387907171 SNP in TYRP1 exhibits strong allele frequency differences among islands in Northern Island Melanesia. Its absence from Bougainville, as well as the weak association with decreased hair color, indicates that additional alleles contribute to the blondism phenotype. PMID: 26450459
2. TYRP1-dependent miR-16 sequestration can also be overcome in vivo by using small oligonucleotides that mask miR-16-binding sites on TYRP1 mRNA. PMID: 28991221
3. The mRNA of TYRP1 is now found to sequester the tumour suppressor miR-16. PMID: 29087386
4. DNA sequencing showed that the patient has carried compound heterozygous mutations of the tyrosinase related protein (TYRP1) gene, namely c.1214C>A (p.T405N) and c.1333dupG, which were inherited from his mother and father, respectively. PMID: 28186599
5. Single nucleotide polymorphisms in TYRP1 gene is associated with multiple primary melanoma. PMID: 25837821
6. Mutation in TYRP1 is associated with oculocutaneous albinism. PMID: 26252096
7. Polymorphisms in 3'UTR of TYRP1 mRNA can affect TYRP1 mRNA regulation by miR-155 and its subsequent translation into protein. These SNPs can render TYRP1 mRNA and protein expression nonsusceptible to miR-155 activity PMID: 26068396
8. These data suggest that UVB-stimulated Ucn1 contributes to TRP1 production via the transcription of both Nurr-1 and Nur77. Ucn1, produced in melanoma cells, acts on melanoma cells themselves in an autocrine manner. PMID: 25240771
9. These data indicate that galectin-3 is a regulatory component in melanin synthesis affecting the expression of Tyrp-1. PMID: 25054620
10. p53 regulation by TRP2 is not pervasive in melanoma. PMID: 24475287
11. Due to mutation in Tyrp1 protein, it became more rigid and might disturb the structural conformation and catalytic function of the structure and might also play a significant role in inducing oculocutaneous albinism type III. PMID: 23862152
12. in human melanoma HMV-II cells both CRF and Ucn1 regulate TRP1 gene expression via Nurr-1/Nur77 production, independent of pro-opiomelanocortin or alpha-melanocyte-stimulating hormone stimulation. PMID: 23416839
13. High TYRP1/S100B mRNA expression in lymph node metastases from melanoma patients is associated with unfavourable clinical outcome. PMID: 23519055
14. We report four Pakistani albinism mutations, including three SLC45A2 alleles and one 22-nucleotide deletion in TYRP1. PMID: 23190901
15. identifed an arginine-to-cysteine change at a highly conserved residue in TYRP1 as a major determinant of blond hair in Solomon Islanders; this missense mutation predicted to affect catalytic activity of TYRP1 and causes blond hair through a recessive mode of inheritance PMID: 22556244
16. We used the first ranked gene, tyrosinase-related protein 1 (TYRP1), further measured its expression in the validation population by real-time PCR and found it to be significantly correlated with distant metastasis-free survival. PMID: 22045183
17. two novel mutations in TYRP1 gene in two Chinese patients with oculocutaneous albinism type 3 PMID: 21739261
18. Case Report: Report a Japanese girl with oculocutaneous albinism type 3 and novel mutations in TYRP1 gene. PMID: 21996312
19. This review focuses on TYRP1, a melanosomal protein involved in the pigmentary machinery of the melanocyte and often used as differentiation marker, with a special emphasis on its emerging roles in the malignant melanocyte and melanoma progression. PMID: 21324755
20. interactions between TRP1-GIPC and GIPC-APPL-AKT provide a potential link between melanogenesis and PI3 kinase signaling PMID: 21291857
21. Adoptive transfer of TRP1-specific T cells into -expressing recipients produces large confluent patches of vitiligo and ocular damage in TRP1-specific T cell receptor transgenic mice. PMID: 20668223
22. Tyrp1 is involved in maintaining stability of tyrosinase protein and modulating its catalytic activity. Tyrp1 is also involved in maintenance of melanosome ultrastructure and affects melanocyte proliferation and melanocyte cell death. PMID: 11775055
23. DNA sequence variants in the human TYRP1 gene are not associated with inherited pigmentary glaucoma in humans. PMID: 12011806
24. We have identified the first TYRP1 mutation in non-Africans and have confirmed that TYRP1 mutations are associated with a milder phenotype of oculocutaneous albinism. PMID: 15996218
25. The presence of a distal Tyrp1 regulatory element, which specifies melanocyte-specific expression, supports the idea that separate regulatory sequences can mediate differential gene expression in melanocytes and RPE. PMID: 16934245
26. Melanocytes in vitiligo demonstrate reduced ability to withstand oxidative stress due, partly, to a disruption in microphthalmia-associated transcription factor regulation of Tyrp1. PMID: 17071589
27. We observe strong evidence for positive selection for TYRP1 alleles in Africans and in Asians PMID: 18312627
28. An eye color variant in TYRP1 was associated with risk of cutaneous melanoma. PMID: 18488027
29. Mutation of TYRP1 (OCA3) can modify the OCA2 phenotype, resulting in red hair. PMID: 18680187
30. Both TRP-1 and galectin-1 were highly expressed in normal melanocytes and melanoma. There was no correlation between TRP-1 or galectin-1 expression and survival. PMID: 19287070
31. TYRP1 rs1408799 is associated with melanoma. PMID: 19384953
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相關疾病：

Albinism, oculocutaneous, 3 (OCA3)
亞細胞定位：

Melanosome membrane; Single-pass type I membrane protein. Melanosome.
蛋白家族：

Tyrosinase family
組織特異性：

Pigment cells.
數據庫鏈接：

HGNC: 12450

OMIM: 115501

KEGG: hsa:7306

STRING: 9606.ENSP00000373570

UniGene: Hs.270279