Recombinant Gloeobacter violaceus Proton-gated ion channel (glvI)

in vitro E.coli expression system

中文名稱：

紫葡萄糖桿菌glvI重組蛋白
貨號：

CSB-CF742273GCI
說明書：

MSDS Datasheet
規格：
來源：

in vitro E.coli expression system
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

基因名：

glvI
Uniprot No.：

Q7NDN8
別名：

glvI; glr4197; Proton-gated ion channel; GLIC; Ligand-gated ion channel; LGIC
種屬：

Gloeobacter violaceus (strain PCC 7421)
蛋白長度：

Full Length of Mature Protein
表達區域：

44-359
氨基酸序列

QDMVSPPPPIADEPLTVNTGIYLIECYSLDDKAETFKVNAFLSLSWKDRRLAFDPVRSGV RVKTYEPEAIWIPEIRFVNVENARDADVVDISVSPDGTVQYLERFSARVLSPLDFRRYPF DSQTLHIYLIVRSVDTRNIVLAVDLEKVGKNDDVFLTGWDIESFTAVVKPANFALEDRLE SKLDYQLRISRQYFSYIPNIILPMLFILFISWTAFWSTSYEANVTLVVSTLIAHIAFNIL VETNLPKTPYMTYTGAIIFMIYLFYFVAVIEVTVQHYLKVESQPARAASITRASRIAFPV VFLLANIILAFLFFGF
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Cationic channel with similar permeabilities for Na(+) and K(+), that is activated by an increase of the proton concentration on the extracellular side. Displays no permeability for chloride ions. Shows slow kinetics of activation, no desensitization and a single channel conductance of 8 pS. Might contribute to adaptation to external pH change.
基因功能參考文獻：
1. Data suggest that neurosteroids bind to two sites per GLIC subunit, both within the transmembrane domain; one site is an intrasubunit site, and the other is located in the interface between subunits. The neurosteroids ligands used in this study were derivatives of 3alpha-hydroxypregnane and tetrahydrodeoxycorticosterone. PMID: 29301936
2. Here we delineate protein motions underlying Gloeobacter violaceus ligand-gated ion channel gating in a membrane environment and report the interface conformation in the closed and the desensitized states. PMID: 24338475
3. X-ray structure of a bacterial ligand-gated ion channel with ketamine bound to an intersubunit cavity that partially overlaps with the homologous antagonist-binding site. PMID: 22958642
4. The overall findings show that His-235 in the transmembrane region of GLIC is a novel proton binding site linked to channel activation. PMID: 22084238
亞細胞定位：

Cell inner membrane; Multi-pass membrane protein.
蛋白家族：

Ligand-gated ion channel (TC 1.A.9) family
數據庫鏈接：

KEGG: gvi:glr4197

STRING: 251221.glr4197