Recombinant Escherichia coli Disulfide bond formation protein B (dsbB)

in vitro E.coli expression system

中文名稱：

大腸桿菌dsbB重組蛋白
貨號：

CSB-CF363883ENV
說明書：

MSDS Datasheet
規格：
來源：

in vitro E.coli expression system
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

基因名：

dsbB
Uniprot No.：

P0A6M2
別名：

dsbB; roxB; ycgA; b1185; JW5182; Disulfide bond formation protein B; Disulfide oxidoreductase
種屬：

Escherichia coli (strain K12)
蛋白長度：

full length protein
表達區域：

1-176
氨基酸序列

MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLCIYERCALFGVLGAALI GAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFATCDFMVRFPEWLPLDKWV PQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLVVISQPFKAKKRDLFGR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by oxidizing the DsbA protein. PhoP-regulated transcription is redox-sensitive, being activated when the periplasm becomes more reducing (deletion of dsbA/dsbB, treatment with dithiothreitol). MgrB acts between DsbA/DsbB and PhoP/PhoQ in this pathway.
基因功能參考文獻：
1. Data find that overexpressing PspE, a periplasmic rhodanese, partially restores substantial disulphide bond formation to a dsbA strain. This activity depends on DsbC, the bacterial disulphide bond isomerase, but not on DsbB. PMID: 22809289
2. We found classes of mutants that specifically affect the apparent K(m) of DsbB for either DsbA or quinones, suggesting that quinone and DsbA may interact with different regions of the DsbB protein. PMID: 15687215
3. characterization of quinone-free forms of DsbB prepared from mutant cells unable to synthesize ubiquinone and menaquinone PMID: 16027117
4. the linker alpha-helix of the bacterial disulfide isomerase DsbC is important for the avoidance of misoxidation by DsbB PMID: 16280324
5. Arg-48 of DsbB in the alpha-helical arrangement is essential for the quinone transition PMID: 16384917
6. These observations suggest that the cytosolic loop is important to coordinate the active-site residues of DsbB and ubiquinone to allow their proper reaction cycles. PMID: 16771666
7. DsbB is converted by its specific substrate, DsbA, to a superoxidizing enzyme, capable of oxidizing this extremely oxidizing oxidase. PMID: 17110337
8. study reports sequential 13C and 15N chemical shift assignments for the majority of the residues in the transmembrane helices PMID: 18227427
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亞細胞定位：

Cell inner membrane; Multi-pass membrane protein.
蛋白家族：

DsbB family
數據庫鏈接：

KEGG: ecj:JW5182

STRING: 316385.ECDH10B_1238