Recombinant Escherichia coli D-methionine transport system permease protein metI (metI)

in vitro E.coli expression system

中文名稱：

大腸桿菌metI重組蛋白
貨號：

CSB-CF339179ENV
說明書：

MSDS Datasheet
規(guī)格：
來源：

in vitro E.coli expression system
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關產(chǎn)品
靶點詳情

產(chǎn)品詳情

基因名：

metI
Uniprot No.：

P31547
別名：

metI; yaeE; b0198; JW0194; D-methionine transport system permease protein MetI
種屬：

Escherichia coli (strain K12)
蛋白長度：

full length protein
表達區(qū)域：

1-217
氨基酸序列

MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPGQIIANAKLYRTVSAIV NIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAPFIARMVENALLEIPTGLI EASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGYSAMGGAVGAGGLGQIGYQYG YIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Part of the binding-protein-dependent transport system for D-methionine and the toxic methionine analog alpha-methyl-methionine. Probably responsible for the translocation of the substrate across the membrane.; (Microbial infection) Probably transports the toxic C-terminal region of CdiA from E.coli strain MHI813 across the inner membrane to the cytoplasm, where CdiA has a toxic effect. Toxin transport is strain-specific, mutations in this gene do not confer resistance to several other tested CdiA toxins.
基因功能參考文獻：
1. crystal structure of high-affinity MetNI methionine uptake transporter was solved to 3.7 angstrom resolution; methionine binds to dimerized carboxyl-terminal domain & inhibits ATPase activity; findings are consistent with allosteric regulatory mechanism PMID: 18621668
亞細胞定位：

Cell inner membrane; Multi-pass membrane protein.
蛋白家族：

Binding-protein-dependent transport system permease family, CysTW subfamily
數(shù)據(jù)庫鏈接：

KEGG: ecj:JW0194

STRING: 316385.ECDH10B_0179