Recombinant Bacillus subtilis Sensor histidine kinase desK (desK)

in vitro E.coli expression system

中文名稱：

枯草芽孢桿菌desK重組蛋白
貨號(hào)：

CSB-CF516538BRJ
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

in vitro E.coli expression system
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

基因名：

desK
Uniprot No.：

O34757
別名：

desK; yocF; BSU19190; Sensor histidine kinase DesK
種屬：

Bacillus subtilis (strain 168)
蛋白長(zhǎng)度：

full length protein
表達(dá)區(qū)域：

1-370
氨基酸序列

MIKNHFTFQKLNGITPYIWTIFFILPFYFIWKSSSTFVIIVGIILTLLFFSVYRFAFVSK GWTIYLWGFLLIGISTASITLFSYIYFAFFIAYFIGNIKERVPFHILYYVHLISAAVAAN FSLVLKKEFFLTQIPFVVITLISAILLPFSIKSRKERERLEEKLEDANERIAELVKLEER QRIARDLHDTLGQKLSLIGLKSDLARKLIYKDPEQAARELKSVQQTARTSLNEVRKIVSS MKGIRLKDELINIKQILEAADIMFIYEEEKWPENISLLNENILSMCLKEAVTNVVKHSQA KTCRVDIQQLWKEVVITVSDDGTFKGEENSFSKGHGLLGMRERLEFANGSLHIDTENGTK LTMAIPNNSK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 10xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Member of the two-component regulatory system DesR/DesK, responsible for cold induction of the des gene coding for the Delta5 acyl-lipid desaturase. Acts as a sensor of the membrane fluidity. Probably activates DesR by phosphorylation.
基因功能參考文獻(xiàn)：
1. The thickness of lipid membranes varies with temperature and that such variations can be detected by DesK with great precision. PMID: 27528507
2. In vivo and biochemical experiments, structural analyses and simulations, demonstrated that stabilization/destabilization of a 2-helix coiled coil is directly involved in regulating DesK's functional state. PMID: 26172072
3. A lipid-mediated conformational switch modulates the thermosensing activity of DesK. PMID: 24522108
4. Data show that the membrane-bound histidine kinase DesK phosphorylates the response regulator DesR to activate des transcription. PMID: 19595746
5. Data from the structural comparison of the different DesK variants indicates that incoming signals can take the form of helix rotations and asymmetric helical bends similar to those reported for other sensing systems. PMID: 19805278
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亞細(xì)胞定位：

Cell membrane; Multi-pass membrane protein.
數(shù)據(jù)庫(kù)鏈接：

KEGG: bsu:BSU19190

STRING: 224308.Bsubs1_010100010591