Recombinant Arabidopsis thaliana Probable steroid reductase DET2 (DET2)

in vitro E.coli expression system

中文名稱：

擬南芥DET2重組蛋白
貨號(hào)：

CSB-CF658520DOA
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

in vitro E.coli expression system
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

基因名：

DET2
Uniprot No.：

Q38944
別名：

DET2; CRO1; At2g38050; T8P21.4; Steroid 5-alpha-reductase DET2; AtDET2; Protein COMPACT ROSETTE 1; Protein DEETIOLATED 2
種屬：

Arabidopsis thaliana (Mouse-ear cress)
蛋白長(zhǎng)度：

full length protein
表達(dá)區(qū)域：

1-262
氨基酸序列

MEEIADKTFFRYCLLTLIFAGPPTAVLLKFLQAPYGKHNRTGWGPTVSPPIAWFVMESPT LWLTLLLFPFGRHALNPKSLLLFSPYLIHYFHRTIIYPLRLFRSSFPAGKNGFPITIAAL AFTFNLLNGYIQARWVSHYKDDYEDGNWFWWRFVIGMVVFITGMYINITSDRTLVRLKKE NRGGYVIPRGGWFELVSCPNYFGEAIEWLGWAVMTWSWAGIGFFLYTCSNLFPRARASHK WYIAKFKEEYPKTRKAVIPFVY
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 10xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Involved in a reduction step in the biosynthesis of the plant steroid, brassinolide; acts at the second step in brassinolide biosynthesis in the 5alpha-reduction of (24R)- 24-methylcholest-4-en-3-one, which is further modified to form campestanol. Can use progesterone, testosterone, androstenedione and campestenone as substrate.
基因功能參考文獻(xiàn)：
1. together with eto1, cesa6(ret8) and det2(ret41) exhibited an enhanced phenotype in the hypocotyls and apical hooks of etiolated seedlings. PMID: 23759102
2. Down-regulation of the DET2:DWF4:SMT2 genes in transgenic Arabidopsis leads to a selective modification of the campesterol and brassinosteroid profiles. PMID: 20169349
3. The isolation and characterization of transient defective exine 1 (tde1), an alleles of DET2, is reported. PMID: 18045813
亞細(xì)胞定位：

Membrane; Multi-pass membrane protein.
蛋白家族：

Steroid 5-alpha reductase family
數(shù)據(jù)庫(kù)鏈接：

KEGG: ath:AT2G38050

STRING: 3702.AT2G38050.1

UniGene: At.22739