Recombinant Arabidopsis thaliana ATP-dependent zinc metalloprotease FTSH 2, chloroplastic (FTSH2)

in vitro E.coli expression system

中文名稱：

擬南芥FTSH2重組蛋白
貨號(hào)：

CSB-CF528047DOA
說(shuō)明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

in vitro E.coli expression system
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

基因名：

FTSH2
Uniprot No.：

O80860
別名：

FTSH2; VAR2; At2g30950; F7F1.16; ATP-dependent zinc metalloprotease FTSH 2, chloroplastic; AtFTSH2; Protein VARIEGATED 2
種屬：

Arabidopsis thaliana (Mouse-ear cress)
蛋白長(zhǎng)度：

Full Length of Mature Protein
表達(dá)區(qū)域：

83-695
氨基酸序列

DEQGVSSSRMSYSRFLEYLDKDRVNKVDLYENGTIAIVEAVSPELGNRVERVRVQLPGLS QELLQKLRAKNIDFAAHNAQEDQGSVLFNLIGNLAFPALLIGGLFLLSRRSGGGMGGPGG PGNPLQFGQSKAKFQMEPNTGVTFDDVAGVDEAKQDFMEVVEFLKKPERFTAVGAKIPKG VLLIGPPGTGKTLLAKAIAGEAGVPFFSISGSEFVEMFVGVGASRVRDLFKKAKENAPCI VFVDEIDAVGRQRGTGIGGGNDEREQTLNQLLTEMDGFEGNTGVIVVAATNRADILDSAL LRPGRFDRQVSVDVPDVKGRTDILKVHAGNKKFDNDVSLEIIAMRTPGFSGADLANLLNE AAILAGRRARTSISSKEIDDSIDRIVAGMEGTVMTDGKSKSLVAYHEVGHAVCGTLTPGH DAVQKVTLIPRGQARGLTWFIPSDDPTLISKQQLFARIVGGLGGRAAEEIIFGDSEVTTG AVGDLQQITGLARQMVTTFGMSDIGPWSLMDSSAQSDVIMRMMARNSMSEKLAEDIDSAV KKLSDSAYEIALSHIKNNREAMDKLVEVLLEKETIGGDEFRAILSEFTEIPPENRVPSST TTTPASAPTPAAV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 10xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Part of a complex that function as an ATP-dependent zinc metallopeptidase. Involved in the thylakoid formation and in the removal of damaged D1 in the photosystem II, preventing cell death under high-intensity light conditions, but not involved in thermotolerance.
基因功能參考文獻(xiàn)：
1. the thylakoid membrane-bound FtsH5 and FtsH2 have dual functions in the maintenance of photosynthetic complexes. PMID: 27208291
2. The initiation of (1)O2 signaling in grana margins depends on EX1 and the ATP-dependent zinc metalloprotease FtsH. As FtsH cleaves also the D1 protein during the disassembly of damaged PSII, EX1- and (1)O2-mediated signaling seems to be not only spatially but also functionally associated with the repair of PSII PMID: 27303039
3. SVR10 and BPG2 are functionally related with VAR2, likely through their potential roles in regulating chloroplast protein homeostasis. PMID: 26435530
4. A brief update of im and var2 and the functions of PTOX and AtFtsH2, is provided. PMID: 23703455
5. The disruption of specific aspects of chloroplast translation, rather than a general impairment in chloroplast translation, suppress var2 variegation and the existence of complex genetic interactions in chloroplast development. PMID: 23721655
6. These data demonstrate in vivo cooperative degradation of D1, in which Deg cleavage assists FtsH processive degradation under photoinhibitory conditions. PMID: 22698923
7. To gain structural information of the possible complexes, the Arabidopsis FtsH2 (type B) and FtsH5 (type A) were modeled. PMID: 22558304
8. Chloroplastic N-terminal methionine excision(cNME) acts upstream of the FtsH protease complex. Developmental defects and higher sensitivity to photoinhibition associated with the ftsh2 mutation were abolished when cNME was inhibited. PMID: 22010036
9. In Arabidopsis thaliana, ATP-dependent chloroplast proteases belonging to FtsH family (especially FtsH2) are considered to be involved in aspects of chloroplast and whole plant maintenance under non-stressing as well as stressing conditions PMID: 21735825
10. The Tat pathway integrates FtsH2 into thylakoids. PMID: 21214651
11. Findings reinforce the strong genetic link between VAR2 and chloroplast translation, and point to a critical role of SVR3, and possibly some aspects of chloroplast translation, in the response of plants to chilling stress. PMID: 21187014
12. The effect of FtsHs in D1 degradation was investigated in Arabidopsis (Arabidopsis thaliana) mutants lacking FtsH2 (yellow variegated2 [var2]) or FtsH5 (var1). PMID: 19767385
13. Results showed that the conserved Gly-Ala-Asp motif plays an important role in FtsH activity and characterization of the var2 alleles could help to identify the physiologically important domain of FtsH. PMID: 15803409
14. A model has been proposed to explain the mechanism of var2 variegation, which suggests that threshold levels of FtsH complexes are required for green sector formation. PMID: 16449381
15. The white sectors in var2 are made by viable cells that have plastids arrested in thylakoid formation. PMID: 17449646
16. These results indicate that photo-oxidative stress by excess light is involved in the regulation of respiratory gene expression and the modulation of respiratory properties, especially the AOX up-regulation. PMID: 18296449
17. Data show that var2-mediated leaf variegation in Arabidopsis can be suppressed by mutations in SUPPRESSOR OF VARIEGATIONS. PMID: 18599582
18. Chloroplast development promoted by the ectopic expression of GPA1 is dependent on FtsH2. PMID: 19228339
顯示更多

收起更多
亞細(xì)胞定位：

Plastid, chloroplast thylakoid membrane; Single-pass membrane protein; Stromal side.
蛋白家族：

AAA ATPase family; Peptidase M41 family
組織特異性：

Expressed in cotyledons, cauline and rosette leaves, stems, sepals, flovers and siliques. Very low in roots.
數(shù)據(jù)庫(kù)鏈接：

KEGG: ath:AT2G30950

STRING: 3702.AT2G30950.1

UniGene: At.22024