HA-Tag Monoclonal Antibody
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中文名稱：HA-Tag鼠單克隆抗體
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貨號：CSB-MA000158
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規格：￥1090
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圖片：
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IP antibody use: 5µg HA Mouse IgG1 per ml Lysate, WB 1:5000
Lane 1: untransfected 293 cell lysate
Lane 2: transfected 293 cell lysate with HA-tag fusion protein
Lane 3: IP (untransfted 293 + anti-HA mAb + Protein G agarose)
Lane 4: IP (transfected 293 + normal Mouse IgG + Protein G agarose)
Lane 5: IP (transfected 293 + anti-HA mAb + Protein G agarose)
Lane 6: IP (transfected 293 + Protein G)
Lane 7: Recombinant protein (E.coli) -
IF analysis of 293 cells transfected with a HA-tag protein,1:2000 dilution (blue DAPI, red anti-HA)
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0.5µg HA fusion protein + Primary antibody dilution at 1:10000
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其他：
產品詳情
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產品描述：本產品為高特異性小鼠源HA-Tag單克隆抗體（CUSABIO貨號：CSB-MA000158），針對來源于流感病毒血凝素（HA）的表位標簽設計，廣泛應用于分子生物學研究中融合蛋白的追蹤與檢測。該抗體經親和層析純化制備，可識別重組蛋白中HA標簽的YPYDVPDYA線性表位，在Western Blot實驗中能有效檢測10-150 kDa范圍內的標記蛋白，免疫熒光實驗適用細胞樣本的定位觀察，免疫沉淀實驗可特異性富集目標蛋白復合物。經嚴格驗證適用于ELISA、WB、IF、IP等實驗體系，其廣泛物種兼容性支持哺乳動物、昆蟲等多種表達系統的HA標簽蛋白檢測。科研人員可將其應用于基因表達調控研究、蛋白互作網絡解析、重組蛋白表達驗證等基礎研究領域，尤其適合需要高靈敏度檢測的轉染細胞或原核表達系統樣本。
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宿主：Mouse
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反應種屬：N/A
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免疫原：Synthetic Peptide
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標記方式：Non-conjugated
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抗體亞型：IgG
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克隆號：26E2
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
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產品提供形式：Liquid
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應用范圍：ELISA, WB, IF, IP
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IF 1:50-1:200 IP 1:200-1:2000 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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