Flag-Tag Monoclonal Antibody
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中文名稱：Flag-Tag鼠單克隆抗體
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貨號：CSB-MA000156
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規(guī)格：￥1090
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圖片：
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IP antibody use: 5µg Flag Mouse IgG1 per ml Lysate, WB 1:5000
Lane 1: untransfected 293 cell lysate
Lane 2: transfected 293 cell lysate with Flag-tag fusion protein
Lane 3: IP (transfected 293 + normal Mouse IgG + Protein G agarose)
Lane 4: IP (transfected 293 + anti- Flag mAb + Protein G agarose)
Lane 5: IP (transfected 293 + Protein G)
Lane 6: Recombinant protein (E.coli) -
IF analysis of 293 cells transfected with a Flag-tag protein,1:2000 dilution (blue DAPI, red anti-Flag)
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1µg Flag fusion protein + Primary antibody dilution at 1:10000
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：CUSABIO貨號：CSB-MA000156 Flag-Tag單克隆抗體是針對Flag短肽標簽開發(fā)的高特異性科研試劑，采用雜交瘤技術(shù)制備，能精準識別重組蛋白中融合的Flag標簽序列（DYKDDDDK）。該抗體經(jīng)嚴格驗證適用于Western Blot、酶聯(lián)免疫吸附（ELISA）、免疫熒光（IF）及免疫共沉淀（IP）等多種實驗體系，其廣泛物種兼容性可滿足哺乳動物、昆蟲細胞等多種表達系統(tǒng)的重組蛋白檢測需求。產(chǎn)品具有高批次穩(wěn)定性，特別適用于靶蛋白的定性分析、亞細胞定位研究以及蛋白相互作用驗證等科研場景，在基因功能研究、信號通路解析和蛋白質(zhì)組學(xué)研究中展現(xiàn)卓越性能。通過優(yōu)化抗原結(jié)合表位，該抗體在保持低背景信號的同時可實現(xiàn)高靈敏度檢測，為研究者提供可靠的Flag標簽融合蛋白追蹤方案，是分子生物學(xué)實驗中重組蛋白表達驗證的核心工具。
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宿主：Mouse
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反應(yīng)種屬：N/A
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免疫原：Synthetic Peptide
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標記方式：Non-conjugated
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抗體亞型：IgG
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克隆號：13C4
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
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產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IF, IP
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IF 1:50-1:200 IP 1:200-1:2000 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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