Recombinant Vaccinia virus Protein F1 (VACWR040)

中文名稱：

Recombinant Vaccinia virus Protein F1 (VACWR040)
貨號：

CSB-YP335035VAI
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

Recombinant Vaccinia virus Protein F1 (VACWR040)
貨號：

CSB-EP335035VAI
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

Recombinant Vaccinia virus Protein F1 (VACWR040)
貨號：

CSB-EP335035VAI-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Vaccinia virus Protein F1 (VACWR040)
貨號：

CSB-BP335035VAI
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Vaccinia virus Protein F1 (VACWR040)
貨號：

CSB-MP335035VAI
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

VACWR040
Uniprot No.：

P24356
別名：

VACWR040; F1LProtein F1
種屬：

Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
蛋白長度：

full length protein
表達區域：

1-226
氨基酸序列

MLSMFMCNNI VDYVDDIDNG IVQDIEDEAS NNVDHDYVYP LPENMVYRFD KSTNILDYLS TERDHVMMAV RYYMSKQRLD DLYRQLPTKT RSYIDIINIY CDKVSNDYNR DMNIMYDMAS TKSFTVYDIN NEVNTILMDN KGLGVRLATI SFITELGRRC MNPVETIKMF TLLSHTICDD YFVDYITDIS PPDNTIPNTS TREYLKLIGI TAIMFATYKT LKYMIG
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Plays a role in evading host innate immune response by inhibiting host inflammasome activation. Interacts with and inhibits NLR-mediated interleukin-1 beta/IL1B production in infected cells. At the host mitochondria, interacts with the BH3 domain of host BAK and prevents BAK from binding active BAX. In turn, host apoptosis is inhibited.
基因功能參考文獻：
1. The data demonstrate that E3 promotes F1 expression by blocking activation of the double-stranded RNA-activated protein kinase R (PKR). PMID: 29997208
2. The N terminus of F1L is not involved in apoptosis inhibition and may act as a regulatory element in other signaling pathways in a manner reminiscent of other unstructured regulatory elements commonly found in mammalian prosurvival Bcl-2 members including Bcl-xL and Mcl-1. PMID: 27151220
3. Authors demonstrate that during vaccinia virus infection, engagement of Bim and Bak by F1L is crucial for subversion of host cell apoptosis. PMID: 24850748
4. F1L protein has a role in virulence of vaccinia virus by inhibiting inflammasome activation PMID: 23603272
5. Viral N1 and F1 do not act as direct cellular Bax inhibitors to prevent Bax-induced cell death. PMID: 22013032
6. analysis of structural determinants of caspase-9 inhibition by the vaccinia virus protein, F1L PMID: 21757755
7. Data suggest that F1L replaces the antiapoptotic activity of Mcl-1 during vaccinia virus infection by interacting with Bak using highly divergent BH domains. PMID: 19955184
8. in addition to interacting with the pro-apoptotic protein Bak, vaccinia F1L also functions to indirectly inhibit the activation of Bax, likely by interfering with the pro-apoptotic activity of BH3-only proteins such as BimL PMID: 17074758
9. Results report that the crystal structure of F1L reveals a Bcl-2-like fold with an unusual N-terminal extension, and that the protein forms a novel domain-swapped dimer in which the alpha1 helix is the exchanged domain. PMID: 18551131
10. Vaccinia growth factor stimulates an epidermal growth factor receptor-MEK-dependent pro-survival pathway that synergizes with F1L to counteract an infection-induced apoptotic pathway that predominantly involves the BH3-only protein Bad. PMID: 19388902
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亞細胞定位：

Host mitochondrion.
蛋白家族：

Poxviridae F1 protein family
數據庫鏈接：

KEGG: vg:3707497