E. coli biotin ligase
(BirA) is highly specific in covalently attaching biotin to the 15
amino
acid AviTag peptide. This recombinant protein was biotinylated in
vivo
by AviTag-BirA technology, which method is BriA catalyzes amide
linkage
between the biotin and the specific lysine of the AviTag.
其他:
在線留言
中文名稱:
Recombinant Tobacco mosaic virus Movement protein(MP)
The tag type will
be
determined during production process. If you have specified tag
type, please tell us and we will develop the specified tag
preferentially.
產品提供形式:
Lyophilized powder
Note: We will
preferentially ship the format that we have in stock, however,
if you have any special requirement for the format, please
remark your requirement when placing the order, we will prepare
according to your demand.
復溶:
We recommend that this vial be briefly centrifuged
prior
to opening to bring the contents to the bottom. Please reconstitute
protein in deionized sterile water to a concentration of 0.1-1.0
mg/mL.We recommend to add 5-50% of glycerol (final concentration)
and
aliquot for long-term storage at -20℃/-80℃. Our default final
concentration of glycerol is 50%. Customers could use it as
reference.
儲存條件:
Store at -20°C/-80°C upon receipt, aliquoting is
necessary for
mutiple use. Avoid repeated freeze-thaw cycles.
保質期:
The shelf life is related to many factors, storage
state,
buffer ingredients, storage temperature and the stability of the
protein
itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C.
The
shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期:
Delivery time may
differ from different purchasing way or location, please kindly
consult your local distributors for specific delivery time.
Note: All of our
proteins are default shipped with normal blue ice packs, if you
request to ship with dry ice, please communicate with us in
advance
and extra fees will be charged.
注意事項:
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet :
Please contact us to get it.
產品評價
靶點詳情
功能:
Transports viral genome to neighboring plant cells directly through plasmosdesmata, without any budding. The movement protein allows efficient cell to cell propagation, by bypassing the host cell wall barrier. Forms a ribonucleoprotein complex with viral RNA. Binds microtubules and modulates microtubule stability. Can bind double-stranded DNA. Triggers host hypersensitive defense reaction in incompatible plants harboring resistance (R) proteins.
基因功能參考文獻:
Data show that ToMV resistance protein Tm-2(2) interacts with Tobacco mosaic virus (TMV) viral movement protein (MP) to form a protein complex at the plasma membrane (PM). PMID: 28258211
The authors identify of a bona fide functional Tobacco mosaic virus MP plasmodesmal localization signal, which encompasses amino acid residues between positions 1 and 50, with residues Val-4 and Phe-14 representing critical sites for function. PMID: 26787834
Arabidopsis (Arabidopsis thaliana) CDC48 is induced upon infection, interacts with MP in endoplasmic reticulum inclusions dependent on the MP N terminus, and promotes degradation of the protein. PMID: 23027663
This Study considered the role of mutations in the transport protein and its binding to viral rna to form a complex. PMID: 15523831
Results indicate that the size difference of necrotic lesions induced by tobacco and tomato mosaic viruses in tobacco plants containing the N gene results from the functional difference of their movement protein genes. PMID: 15620106
During viral infection in Tobacco, protein is phosphorylated. PMID: 15680421
the function of movement protein in TMV viral movement may involve interaction with the microtubule-nucleating machinery. PMID: 16731920
functions as a genuine microtubule-associated protein that binds microtubules directly and modulates microtubule stability. PMID: 16912284
TMV exhibits the activity of a suppressor of viral RNA silencing. PMID: 16986444
Data supports a diffusion model in which a complex that includes endoplasmic reticulum (ER)-embedded movement protein, viral RNA, and other components diffuses in the ER membrane within the plasmodesmata. PMID: 18257683
microtubule network performs anchorage and release functions for controlling the assembly and intracellular movement of movement protein-containing RNA transport particles in association with the endoplasmic reticulum PMID: 19281527
NtMPIP1, a novel subset of DnaJ-like proteins, was identified from a tobacco cDNA library, and its specific interaction with TMV MP was confirmed with an in vitro filter-binding assay. PMID: 19458900