Recombinant Staphylococcus aureus Alpha-hemolysin (hly)

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中文名稱：

金黃色釀膿葡萄球菌hly重組蛋白
品名簡稱：

Recombinant Staphylococcus aureus hly protein
貨號：

CSB-YP639324FLF
說明書：

MSDS Datasheet
規格：

￥2616
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP639324FLF could indicate that this peptide derived from Yeast-expressed Staphylococcus aureus (strain NCTC 8325) hly.

Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP639324FLF could indicate that this peptide derived from Yeast-expressed Staphylococcus aureus (strain NCTC 8325) hly.
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產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

hly
Uniprot No.：

Q2G1X0
別名：

hly; hla; SAOUHSC_01121Alpha-hemolysin; Alpha-HL; Alpha-toxin
種屬：

Staphylococcus aureus (strain NCTC 8325)
蛋白長度：

Full Length of Mature Protein
來源：

Yeast
分子量：

35.3kDa
表達區域：

27-319aa
氨基酸序列

ADSDINIKTGTTDIGSNTTVKTGDLVTYDKENGMHKKVFYSFIDDKNHNKKLLVIRTKGTIAGQYRVYSEEGANKSGLAWPSAFKVQLQLPDNEVAQISDYYPRNSIDTKEYMSTLTYGFNGNVTGDDTGKIGGLIGANVSIGHTLKYVQPDFKTILESPTDKKVGWKVIFNNMVNQNWGPYDRDSWNPVYGNQLFMKTRNGSMKAADNFLDPNKASSLLSSGFSPDFATVITMDRKASKQQTNIDVIYERVRDDYQLHWTSTNWKGTNTKDKWIDRSSERYKIDWEKEEMTN
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

Recombinant full-length of Mature Staphylococcus aureus Alpha-hemolysin (hly) Protein (27-319 amino acids) with a 6xHis tag at the C-terminus was expressed in the yeast. SDS-PAGE analysis of the purified hly protein gave one band at 34–40 kDa of the target molecule. The purity of this hly protein is greater than 90%. The identification of the hly protein was validated by the LC-MS/MS Analysis. The delivery time is relatively short because the hly protein is in-stock.

Alpha-hemolysin (hly), also known as hla, is a single component toxin generated by Staphylococcus aureus, a potential human causative agent that can cause various acute and chronic infections. Hly plays an important role in the pathogenesis of Staphylococcus aureus. The interaction between hly and the specific cell receptor ADAM10 forms cylindrical transmembrane heptameric pores. The resulting pores allow the influx and efflux of ions and small molecules, ultimately leading to disruption of ionic equilibrium, osmotic expansion, and cell death.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Alpha-toxin binds to the membrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. Inhibits host neutrophil chemotaxis to the lesion region (Probable). Heptamer oligomerization and pore formation is required for lytic activity.
基因功能參考文獻：
1. We now show that ADAM10 is critical for alpha-hemolysin-mediated activation of the NLRP3 inflammasome in human monocytes as siRNA knockdown or chemical blockade of ADAM10-alpha-hemolysin interaction leads to diminished inflammasome activation and cell death by reducing the available ADAM10 on the cell surface. PMID: 27043625
2. Staphylococcus aureus hemolysin A disrupts cell-matrix adhesions in human airway epithelial cells. PMID: 24918472
3. Succeeded in preparing crystals of the heptameric form of alpha-hemolysin without any detergent but with 2-methyl-2,4-pentanediol (MPD), and determined its structure. PMID: 21280135
4. Staphylococcus aureus alpha-hemolysin activates the NLRP3-inflammasome in human and mouse monocytic cells PMID: 19826485
5. The wide vestibule leading into the alpha-hemolysin pore helps to orient negatively charged synthetic alpha-helical peptides for translocation. PMID: 16866363
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亞細胞定位：

Secreted.
蛋白家族：

Aerolysin family
數據庫鏈接：

KEGG: sao:SAOUHSC_01121

STRING: 93061.SAOUHSC_01121