Recombinant Spindle and kinetochore-associated protein 1 homolog (CBG02218)
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中文名稱：布里格薩隱桿線蟲ska-1重組蛋白
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貨號：CSB-EP427099CXX-B
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說明書：
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規(guī)格：
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來源：E.coli
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共軛：Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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其他：
產(chǎn)品詳情
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純度：>85% (SDS-PAGE)
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基因名：ska-1
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Uniprot No.：
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別名：ska-1; CBG02218; Spindle and kinetochore-associated protein 1 homolog
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種屬：Caenorhabditis briggsae
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蛋白長度：full length protein
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表達區(qū)域：1-239
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氨基酸序列MEAFMVSFER QKDAKFCMYR ENIPNFESTL SSITKSTNSI KILLEDISNV QKVITDPCEQ SCSSCSPNVL NKFLGIEEKL VNNVEIPDSV PQKSTRPCLD DEKEGSSVVQ PPESGNRHVQ LISEQEFKSI PKYQLGRLTL DNLNEIVGKM DEFLTKKNAI LGKTNKQITR QDREVLDNWR ELEIKAKGRL PTTLFFIEND IRPLLTERLR LSFAKAIPCL RHIRRVREER CGPLTFYYA
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蛋白標(biāo)簽：Tag?type?will?be?determined?during?the?manufacturing?process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
產(chǎn)品提供形式：Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
復(fù)溶：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲存條件：Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質(zhì)期：The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期：Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項：Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet ：Please contact us to get it.
產(chǎn)品評價
靶點詳情
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功能：Component of the ska-1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The ska-1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules. In the complex, it mediates the interaction with microtubules.
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亞細(xì)胞定位：Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore.
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蛋白家族：SKA1 family
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數(shù)據(jù)庫鏈接：
KEGG: cbr:CBG02218
STRING: 6238.CBG02218
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