Recombinant Schizosaccharomyces pombe NEDD8-specific protease 1 (nep1)

中文名稱：

龐貝裂殖酵母nep1重組蛋白
貨號：

CSB-YP527315SXV
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

龐貝裂殖酵母nep1重組蛋白
貨號：

CSB-EP527315SXV
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

龐貝裂殖酵母nep1重組蛋白
貨號：

CSB-EP527315SXV-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

龐貝裂殖酵母nep1重組蛋白
貨號：

CSB-BP527315SXV
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

龐貝裂殖酵母nep1重組蛋白
貨號：

CSB-MP527315SXV
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

nep1
Uniprot No.：

O42980
別名：

nep1; SPBC17D11.01; SPBC20F10.11; NEDD8-specific protease 1; EC 3.4.22.-
種屬：

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
蛋白長度：

full length protein
表達區域：

1-420
氨基酸序列

MSSSPTVLEL FDVCFKQEDV DSLKKPNWFT DVSIDYVDEL IEHLWFPSYP NQANEILLLR PSLVFLLAEA AISPEELKVA LPKKLMNCKY LFMPINDLDK HAAGSGGSHW SLMVASIPDG QCYYYDSLSN GKTKDCRSAL ARVSDLFKKK FTIECMPVQQ QRNGYDCGAH VCAFTLELVR RLLHSPMPTS SMWNLSTFQP DVTAIREQLS RCLDHIINSL GTRVSGDFDE DFPTGTVFFD LESHLPLLDV ALPVLPKSSD SSETSHESSN SNLKKSSESG STNHHNNHES DKDLHHEGHH HHHHHHHHHH SHDDDPSSPA EKKQNHVPSP SEKIQDHVPS PSEKKQDRVP SPSNNKEDHL PLLSDEKLDK SAIDKIEPTP LPSVHMNSHI AKGELPKFHN STDNPFLTPP EELVSGDFPF
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Protease that catalyzes two essential functions in the NEDD8 pathway: processing of full-length NEDD8 to its mature form and deconjugation of NEDD8 from targeted proteins such as the pcu1, pcu2 and pcu4 cullins and other proteins.
基因功能參考文獻：
1. The isolation and characterization of Nep1, a deneddylating enzyme, are reported. Although Nep1 can deneddylate cullins in vitro, its in vivo activity is probably directed against other neddylated targets. [Nep1] PMID: 15769255
亞細胞定位：

Cytoplasm.
蛋白家族：

Peptidase C48 family
數據庫鏈接：

KEGG: spo:SPBC17D11.01

STRING: 4896.SPBC17D11.01.1