Recombinant Saccharomyces cerevisiae Spindle assembly checkpoint component MAD3 (MAD3), partial

中文名稱：

釀酒酵母MAD3重組蛋白
貨號：

CSB-YP342455SVG
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

釀酒酵母MAD3重組蛋白
貨號：

CSB-EP342455SVG
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

釀酒酵母MAD3重組蛋白
貨號：

CSB-EP342455SVG-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

釀酒酵母MAD3重組蛋白
貨號：

CSB-BP342455SVG
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

釀酒酵母MAD3重組蛋白
貨號：

CSB-MP342455SVG
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

MAD3
Uniprot No.：

P47074
別名：

MAD3; YJL013C; J1341Spindle assembly checkpoint component MAD3; Mitotic MAD3 protein
種屬：

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
蛋白長度：

Partial
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點(diǎn)詳情

功能：

Component of the spindle assembly checkpoint which is a feedback control that prevents cells with incompletely assembled spindles from leaving mitosis. Component of the mitotic checkpoint complex (MCC) which inhibits the ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) by preventing its activation by CDC20.
基因功能參考文獻(xiàn)：
1. our results are consistent with a role of Mad3 as the main interactor of Mad2 via the dimerization surface. Mad3 being required to stabilize the Cdc20:C-Mad2 complex implies that the complex by itself is unstable. PMID: 23000150
2. In vivo Mad3 phosphorylation depends on the Polo kinase Cdc5 and, to a minor extent, the Aurora B kinase Ipl1. PMID: 15970700
3. Ipl1p-dependent phosphorylation of Mad3p is required for the spindle checkpoint response to lack of tension at kinetochores. PMID: 17504936
亞細(xì)胞定位：

Nucleus.
數(shù)據(jù)庫鏈接：

KEGG: sce:YJL013C

STRING: 4932.YJL013C