Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase RAD53 (RAD53), partial

中文名稱：

釀酒酵母RAD53重組蛋白
貨號(hào)：

CSB-YP322114SVG
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

Yeast
其他：

中文名稱：

釀酒酵母RAD53重組蛋白
貨號(hào)：

CSB-EP322114SVG
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

E.coli
其他：

中文名稱：

釀酒酵母RAD53重組蛋白
貨號(hào)：

CSB-EP322114SVG-B
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

釀酒酵母RAD53重組蛋白
貨號(hào)：

CSB-BP322114SVG
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

Baculovirus
其他：

中文名稱：

釀酒酵母RAD53重組蛋白
貨號(hào)：

CSB-MP322114SVG
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

RAD53
Uniprot No.：

P22216
別名：

CHEK2 homolog; CHK2 homolog; MEC2; RAD53; RAD53_YEAST; Serine protein kinase 1; Serine-protein kinase 1; Serine/threonine-protein kinase RAD53; SPK1
種屬：

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
蛋白長(zhǎng)度：

Partial
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Controls S-phase checkpoint as well as G1 and G2 DNA damage checkpoints. Phosphorylates proteins on serine, threonine, and tyrosine. Prevents entry into anaphase and mitotic exit after DNA damage via regulation of the Polo kinase CDC5. Seems to be involved in the phosphorylation of RPH1.
基因功能參考文獻(xiàn)：
1. Rad53 prevents the generation of excessive ssDNA under replication stress by coordinating DNA unwinding with synthesis of both strands. PMID: 29033319
2. Rad53 can display active conformations and phosphor-priming confers functionally relevant autophosphorylation. PMID: 28858528
3. H2B-mediated Rad53 activation promotes the dissociation of histone chaperone Asf1 from the Rad53 complex. PMID: 25275495
4. Functional relevance of the Rad53 dimer as observed in our crystal structure, and reveal the importance of this mode of head-to-tail, face-to-face dimerization for Rad53 activation. PMID: 24815189
5. Mechanisms of phospho-priming and auto-activation of the checkpoint kinase Rad53 in vivo. PMID: 24302356
6. The overexpression led to partial activation of Rad53, uncovering that the negative impacts of Rad53 kinase activation on meiotic progression, and formation and interhomolog repair of meiotic programmed DNA double-strand breaks. PMID: 24183725
7. Rad53 regulates a transcriptional response to DNA damage involving more than 600 genes. PMID: 23810556
8. rad53 physical interaction with Dbf4 is required gor Dbf4 phosphorylation for replication checkpoint activation. PMID: 23564203
9. Even in the absence of DNA-damaging agents, the rad53-4AQ mutant, lacking the N-terminal Mec1 phosphorylation site cluster, is synthetic lethal with a deletion of the RAD9 DNA damage checkpoint adaptor. PMID: 23754745
10. The existence of a Rad53p-regulated checkpoint that regulates G1- to S-phase progression in response to loss of mtDNA. PMID: 22927468
11. The role of Rad53 is in degradation of excess cellular histone and in replication initiation are related. PMID: 21099362
12. Threshold levels of Rad53 activity modulate the tolerance to alkylating DNA damage at least by controlling the abundance of the key TLS factor Rev1 bound to chromatin. PMID: 20674515
13. Rad53 is phosphorylated independently of the DNA damage checkpoint response in a cell cycle dependent manner. PMID: 20046099
14. Required for double-strand break-initiated sister chromatin exchange (SCE) and DNA damage-associated SCE after exposure to X-ray-mimetic and ultraviolet-mimetic chemicals. PMID: 16039914
15. A novel role for Rad53 in the initiation of DNA replication that is independent of checkpoint or deoxynucleotide regulation. PMID: 16816422
16. Collectively, the results suggest a novel function of Rad53 in the regulation of polarized cell growth in response to DNA replication stress. PMID: 17130285
17. forkhead-associated domain 1 (FHA1) of Rad53 interacts with a specific threonine of Ptc2, T376 PMID: 17325030
18. Rad53/Chk2 homo-oligomerization is an evolutionarily conserved mechanism that drives Rad53/Chk2 activation and promotes signal amplification in DNA damage responses. PMID: 18239457
19. 3 rad53 alleles in which FHA1 & FHA2 domains were individually or simultaneously transposed to the opposite location. All three mutants were approximately 100-fold hypersensitive to DNA lesions whose survival requires intact Rad53 FHA domain functions. PMID: 18302321
20. distinct phosphatases operate to return Rad53 to its basal state after different genotoxic stresses and a yet unidentified phosphatase may be responsible for the deactivation of Rad53 after replication stress PMID: 18441009
21. These data indicate that Rad53 prevents sister chromatid segregation in the presence of unrepaired programmed meiotic DSBs, thus providing a salvage mechanism ensuring genetic integrity in the gametes even in the absence of the recombination checkpoint. PMID: 18505828
22. Dun1 shows increased affinity for diphosphoyrlated Rad53 cluster domains due to the presence of two separate phosphothreonine-binding pockets. PMID: 18570878
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亞細(xì)胞定位：

Nucleus.
蛋白家族：

Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CHEK2 subfamily
數(shù)據(jù)庫(kù)鏈接：

KEGG: sce:YPL153C

STRING: 4932.YPL153C