Recombinant Saccharomyces cerevisiae Nucleoporin POM152 (POM152), partial
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中文名稱:釀酒酵母POM152重組蛋白
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貨號(hào):CSB-EP340137SVG-B
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說明書:
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規(guī)格:
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來源:E.coli
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共軛:Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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其他:
產(chǎn)品詳情
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純度:>85% (SDS-PAGE)
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基因名:POM152
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Uniprot No.:
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別名:POM152; YMR129W; YM9553.05; Nucleoporin POM152; Nuclear pore protein POM152; P150; Pore membrane protein POM152
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種屬:Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
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蛋白長(zhǎng)度:Partial
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蛋白標(biāo)簽:Tag?type?will?be?determined?during?the?manufacturing?process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
產(chǎn)品提供形式:Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
復(fù)溶:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲(chǔ)存條件:Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質(zhì)期:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期:Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項(xiàng):Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet :Please contact us to get it.
靶點(diǎn)詳情
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功能:Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. POM152 is important for the de novo assembly of NPCs.
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基因功能參考文獻(xiàn):
- Study presents the integrative molecular architecture of Pom152, the major component of the nuclear pore complex (NPC) membrane ring. Pom152 is formed by a flexible string-of-pearls arrangement of nine repetitive cadherin-like Ig-like domains, indicating an evolutionary connection between NPCs and the cell adhesion machinery. PMID: 28162953
- Truncations of the N-glycosylated, lumenal domain of Pom152p and pom152 mutants lacking N-linked glycosylation sites are viable in combination with nup1Delta, and partially suppress the nuclear protein import defects associated with the deletion of NUP1. PMID: 16118201
- The absence of Pom152p results in aberrant nuclear pores that have enlarged diameters and lack proteinaceous material, leading to an increased diffusion between the cytoplasm and the nucleus. PMID: 16682526
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亞細(xì)胞定位:Nucleus, nuclear pore complex. Nucleus membrane; Multi-pass membrane protein. Note=Central core structure of the nuclear pore complex.
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數(shù)據(jù)庫(kù)鏈接:
KEGG: sce:YMR129W
STRING: 4932.YMR129W
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